Abstract：
PURPOSE:To investigate the potential of chitosan nanoparticles as a system for improving the systemic absorption of insulin following nasal instillation. METHODS:Insulin-loaded chitosan nanoparticles were prepared by ionotropic gelation of chitosan with tripolyphosphate anions. They were characterized for their size and zeta potential by photon correlation spectroscopy and laser Doppler anemometry, respectively. Insulin loading and release was determined by the microBCA protein assay. The ability of chitosan nanoparticles to enhance the nasal absorption of insulin was investigated in a conscious rabbit model by monitoring the plasma glucose levels. RESULTS:Chitosan nanoparticles had a size in the range of 300-400 nm, a positive surface charge and their insulin loading can be modulated reaching values up to 55% [insulin/nanoparticles (w/w): 55/100]. Insulin association was found to be highly mediated by an ionic interaction mechanism and its release in vitro occurred rapidly in sink conditions. Chitosan nanoparticles enhanced the nasal absorption of insulin to a greater extent than an aqueous solution of chitosan. The amount and molecular weight of chitosan did not have a significant effect on insulin response. CONCLUSIONS:Chitosan nanoparticles are efficient vehicles for the transport of insulin through the nasal mucosa.
journal_name
Pharm Resjournal_title
Pharmaceutical researchauthors
Fernández-Urrusuno R,Calvo P,Remuñán-López C,Vila-Jato JL,Alonso MJdoi
10.1023/a:1018908705446subject
Has Abstractpub_date
1999-10-01 00:00:00pages
1576-81issue
10eissn
0724-8741issn
1573-904Xjournal_volume
16pub_type
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journal_title：Pharmaceutical research
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