Abstract:
:1. Modulation by nitric oxide (NO) of the ATP-sensitive K+ channel (KATP) current (IK(ATP)) was investigated in single ventricular cells dissociated from guinea-pig hearts. IK(ATP) was induced by 5-amino-N-[2-(2-chlorophenyl)ethyl]-N'-cyano-3-pyridinecarboxamidine+ ++ (KRN4884) and cromakalim. 2. In the whole-cell patch clamp configuration, KRN4884 (0.1 3 microM) increased the outward current in a concentration-dependent manner with an EC50 value of 0.48 microM. This current was completely antagonized by glibenclamide (1 microM). 3. IK(ATP) induced by either KRN4884 (0.3 microM) or cromakalim (10 microM) was significantly enhanced by the additional application of a NO donor (+/-)-(E)-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexeneamide (NOR3, 0.1 and 1 mM). 4. The potentiating effect was observed only when NOR3 solution was prepared just before experiments, when release of NO was considered to be on-going. The effect was significantly eliminated in the presence of the NO scavenger oxyhaemoglobin (3 10 microM). Also, oxidative metabolites of NO, such as NO2 or NO3, were without effect. 5. 8-Bromo-guanosine-3':5'-cyclic monophosphate (8-Br-cyclic GMP, 0.1 0.5 mM) significantly decreased IK(ATP) induced by KRN4884. 6. In cell-attached patches, NOR3 (1 mM) potentiated the KRN4884-induced IK(ATP) in a way similar to that seen in whole-cell recordings. By contrast, NOR3 (1 mM) did not enhance the current in either inside-out or outside-out patches. 7. These results indicate that NO potentiates the action of K+ channel openers on the KATP through a mechanism which remains to be determined.
journal_name
Br J Pharmacoljournal_title
British journal of pharmacologyauthors
Shinbo A,Iijima Tdoi
10.1038/sj.bjp.0701069subject
Has Abstractpub_date
1997-04-01 00:00:00pages
1568-74issue
8eissn
0007-1188issn
1476-5381journal_volume
120pub_type
杂志文章abstract::1. Sarcolemmal membranes were isolated from normal and from denervated muscles and the specific binding of [125I]-alpha-bungarotoxin to the membranes was determined. 2. Cytosol prepared from either slow (soleus) or fast (extensor digitorum longus) muscle increased the toxin binding. Similar effects were seen with cyto...
journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1988.tb11611.x
更新日期:1988-07-01 00:00:00
abstract:UNLABELLED:Increasing evidence suggests that an overactive endocannabinoid system (ECS) may contribute to the development of diabetes by promoting energy intake and storage, impairing both glucose and lipid metabolism, by exerting pro-apoptotic effects in pancreatic beta cells and by facilitating inflammation in pancre...
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journal_title:British journal of pharmacology
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doi:10.1111/j.1476-5381.1990.tb12968.x
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abstract::1. An obligatory step in the biosynthesis of endothelin-1 (ET-1) is the conversion of its inactive precursor, big ET-1, into the mature form by the action of specific, phosphoramidon-sensitive, endothelin converting enzyme(s) (ECE). Disparate effects of big ET-1 and ET-1 on renal tubule function suggest that big ET-1 ...
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更新日期:1997-02-01 00:00:00
abstract::1. The stimulatory effect of neuropeptide Y (NPY) on food intake is well established but the roles of the receptor subtypes Y(1) and Y(5) have been difficult to define. We have studied the effects of two novel Y(1)-preferring and two Y(5)-preferring agonists on feeding in guinea pigs. 2. The Y(1)-preferring receptor a...
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doi:10.1038/sj.bjp.0702896
更新日期:1999-11-01 00:00:00
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journal_title:British journal of pharmacology
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journal_title:British journal of pharmacology
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doi:10.1038/sj.bjp.0701932
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更新日期:2004-02-01 00:00:00
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abstract::1. This study was done to characterize the influence of calcium channel blockade on renin secretion and renin gene expression in normal rats and rats with renovascular hypertension. To this end we studied the effects of the 1,4-dihydropyridine derivative, amlodipine, on plasma renin activity and renal renin m-RNA leve...
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pub_type: 杂志文章
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更新日期:1996-10-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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abstract::1 Prolonged exposure (6-12 h) of rat aorta to alpha1-adrenergic receptor (alpha(1)AR) agonist phenylephrine (Phe) leads to a decrease in alpha(1)AR-mediated vasoconstriction. This reduced responsiveness to alpha(1)AR stimulation was strongly dependent on the intactness of the endothelium. 2 We examined the effect of P...
journal_title:British journal of pharmacology
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journal_title:British journal of pharmacology
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pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:1992-08-01 00:00:00
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更新日期:2015-12-01 00:00:00
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更新日期:2011-09-01 00:00:00