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Enzymatic synthesis of S-adenosyl-L-methionine on the preparative scale.

Abstract：

:The problems inherent in the enzymatic and chemical synthesis of S-adenosyl-L-methionine (SAM) led us to develop an efficient, simple method for the synthesis of large amounts of labeled SAM. Previously, we reported that the problem of product inhibition of E. coli SAM synthetase encoded by the metK gene was successfully overcome in the presence of sodium p-toluenesulfonate (pTsONa). This research has now been expanded to demonstrate that product inhibition of this enzyme can also be overcome by adding a high concentration of beta-mercaptoethanol (beta ME), acetonitrile, or urea. In addition a recombinant strain of E. coli has been constructed that expresses the yeast SAM synthetase encoded by the sam2 gene. The yeast enzyme does not have the problem of product inhibition seen with the E. coli enzyme. Complete conversion of 10 mM methionine to SAM was achieved in incubations with either the recombinant yeast enzyme and 1 molar potassium ion or the E. coli enzyme in the presence of additives such as beta ME, acetonitrile, urea, or pTsONa. The recombinant yeast SAM synthetase was used to generate SAM in situ for use in the multi-enzymatic synthesis of precorrin 2.

journal_name

Bioorg Med Chem

journal_title

Bioorganic & medicinal chemistry

authors

Park J,Tai J,Roessner CA,Scott AI

doi

10.1016/s0968-0896(96)00228-3

subject

Has Abstract

pub_date

1996-12-01 00:00:00

pages

2179-85

issue

12

eissn

0968-0896

issn

1464-3391

pii

S0968-0896(96)00228-3

journal_volume

4

pub_type

杂志文章

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