A rat model for investigation of bladder dysfunction associated with demyelinating disease resembling multiple sclerosis.

Abstract:

:Myelin basic protein (MBP) can be used as an antigen for inducing experimental allergic encephalomyelitis (EAE). In various studies, EAE animals have been used as an experimental model of demyelinating diseases. The aim of this study was to determine whether EAE, induced by MBP in rats, can be useful for investigation of bladder dysfunction associated with demyelinating disease. Female Lewis rats were used. In Study 1, the time course of behavioral and cystometric changes were observed consecutively after MBP sensitization. In Study 2, the correlations between behavioral, cystometric, and histologic abnormalities were studied. The degree of paralysis and histologic findings were evaluated. In Study 1, transient hind limb paralysis was observed in all rats. Cystometric findings were characterized by three different patterns: 1) detrusor areflexia (DA), 2) detrusor hyperactivity (DH), and 3) normal. Ten (77%) of the 13 rats given MBP showed bladder dysfunction, including DA (seven), DA/DH (two) and DH (one). Study 2 showed DA in 10 rats, DH in one, and normal findings in nine animals. The difference in degree of paralysis between the DA and the cystometrically normal animals was statistically significant (P<0.01). The mean value of the degree of inflammation in the spinal cord (L6-S1) in the DA group was significantly (P<0.05) higher than that in the cystometrically normal group. The degrees of paralysis and spinal inflammation were weakly correlated (R = 0.47, P = 0.05). The present rat model seems useful for studies of bladder dysfunction associated with spinal myelitis/demyelinating diseases.

journal_name

Neurourol Urodyn

authors

Mizusawa H,Igawa Y,Nishizawa O,Ichikawa M,Ito M,Andersson KE

doi

10.1002/1520-6777(2000)19:6<689::aid-nau7>3.0.co;2

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

689-99

issue

6

eissn

0733-2467

issn

1520-6777

pii

10.1002/1520-6777(2000)19:6<689::AID-NAU7>3.0.CO;2

journal_volume

19

pub_type

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