Abstract:
:Removal of the embryonic axis prevents the normal decline of carboxypeptidase (Cpase) I in mung bean seedling cotyledons. Cpase I activity and protein, the latter manifested on western blots, almost completely disappear about 24 h before the cotyledon abscises. Of the 3 proteolytic enzyme patterns, only that of Cpase I can be restored by an exogenous supply of 10 mM CaCl2 in the agar growth medium. The calcium effect is dependent on [CaCl2] and is not manifested in the presence of chelators and calcium channel blockers. For detached cotyledons to show the normal low level of Cpase I by the eighth day of growth, calcium had to be supplied during seed imbibition and throughout the entire time from removal of the axis. The difference between detached cotyledons in the absence and presence of calcium was greatest when the cotyledons were detached 4-6 days after seed imbibition. Loss of Cpase I activity and protein can be demonstrated in vitro, with the maximum level of Cpase I-degrading activity measured 4 days after seed imbibition under the same growth conditions used to study the calcium effect. It is sensitive to pepstatin and has a pH optimum of 3, suggesting that this Cpase I-degrading activity is due to an aspartic protease.
journal_name
Physiol Plantjournal_title
Physiologia plantarumauthors
Singh S,Wilson KA,Tan-Wilson ALdoi
10.1034/j.1399-3054.2001.1120306.xsubject
Has Abstractpub_date
2001-07-01 00:00:00pages
334-342issue
3eissn
0031-9317issn
1399-3054pii
ppl1120306journal_volume
112pub_type
杂志文章abstract::The gravitropic curvature of seedlings of lentil (Lens culunaris L. cv. Verte du Puy) grown in microgravity and stimulated on the 1 g centrifuge for 5 to 60 min was followed by time lapse photography in near weightlessness in the frame of the IML 1 Mission of Spacelab. In microgravity, the root tip could overshoot the...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
abstract::Calreticulin (CRT) is a key Ca2+-binding protein mainly resident in the endoplasmic reticulum (ER), which is highly conserved and extensively expressed in all eukaryotic organisms investigated. The protein plays important roles in a variety of cellular processes including Ca2+ signaling and protein folding. Although c...
journal_title:Physiologia plantarum
pub_type: 杂志文章,评审
doi:10.1111/j.1399-3054.2009.1223.x
更新日期:2009-06-01 00:00:00
abstract::Maintaining a high rate of water uptake is crucial for maximum longevity of cut stems. Physiological gel/tylosis formation decreases water transport efficiency in the xylem. The primary mechanism of action for post-harvest Cu(2+) treatments in improving cut flower and foliage longevity has been elusive. The effect of ...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12028
更新日期:2013-08-01 00:00:00
abstract::Shoot-root communication is involved in plant stress responses, but its mechanism is largely unknown. To determine the role of roots in stress tolerance, cucumber (Cucumis sativus) shoots from plants with roots of their own or with figleaf gourd (Cucurbita ficifolia, a chilling-tolerant species) or luffa (Luffa cylind...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12200
更新日期:2014-11-01 00:00:00
abstract::Two mutants of Arabidopsis thaliana deficient in lutein have been investigated with respect to their responses to growth under a range of suboptimal conditions. The first mutant, lut1, was enriched in violaxanthin, antheraxanthin, zeaxanthin and zeinoxanthin compared with the wild-type (WT). In the second mutant, lut2...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2008.01124.x
更新日期:2008-09-01 00:00:00
abstract::Intracellular selective proteolysis is an important post-translational regulatory mechanism maintaining protein quality control by removing defective, damaged or even deleterious protein aggregates. The ATP-dependent Lon protease is a key component of protein quality control that is highly conserved across the kingdom...
journal_title:Physiologia plantarum
pub_type: 杂志文章,评审
doi:10.1111/j.1399-3054.2011.01537.x
更新日期:2012-05-01 00:00:00
abstract::The final contents of primary and secondary metabolites of the ripe fruit depend on metabolic processes that are tightly regulated during fruit ontogeny. Carbohydrate supply during fruit development is known to influence these processes but, with respect to secondary metabolites, we do not really know whether this inf...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2012.01672.x
更新日期:2013-04-01 00:00:00
abstract::Arabidopsis CONSTITUTIVE PHOTOMORPHOGENIC1/SUPPRESSOR OF PHYA-105 (COP1/SPA) is an E3 ubiquitin ligase complex that prevents photomorphogenesis in darkness by ubiquitinating and subsequently degrading light-responsive transcription factors. Upon light perception, photoreceptors directly interact with the COP1/SPA comp...
journal_title:Physiologia plantarum
pub_type: 杂志文章,评审
doi:10.1111/ppl.13103
更新日期:2020-07-01 00:00:00
abstract::The 'stay-green' mutation cytG in soybean (Glycine max) partially inhibits the degradation of the light-harvesting complex II (LHCII) and the associated chlorophyll during monocarpic senescence. cytG did not alter the breakdown of the cytochrome b6/f complex, thylakoid ATP synthase or components of Photosystem I. In c...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1034/j.1399-3054.2002.1150317.x
更新日期:2002-07-01 00:00:00
abstract::The enormous biological diversity of endophytes, coupled with their potential to enhance the production of bioactive metabolites in plants, has driven research efforts focusing on endophytes. However, limited information is available on the impacts of bacterial endophytes on plant secondary metabolism and signaling pa...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12236
更新日期:2015-01-01 00:00:00
abstract::With the objective to clarify the physiological significance of polyamines (PAs) in the photosynthetic apparatus, the present study investigated the effects of salt stress with and without foliar application of putrescine (Put) on the structure and function of the photosynthetic apparatus in cucumber. Salt stress at 7...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2012.01623.x
更新日期:2012-11-01 00:00:00
abstract::The aim was to determine cadmium (Cd) speciation in various plants, between buffer-soluble and acid-soluble Cd, and also within the buffer-soluble Cd. A better understanding of Cd speciation shows the relative importance of different biological mechanisms for Cd sequestration. Roots of Pistia stratiodes, Eichhornia cr...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2007.00960.x
更新日期:2007-10-01 00:00:00
abstract::A better understanding of somatic embryogenesis in banana (Musa spp.) may provide a practical way to improve regeneration of banana plants. In this study, we applied scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to visualize the ultrastructural changes during somatic embryogenesis of ba...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2011.01478.x
更新日期:2011-08-01 00:00:00
abstract::Sequestration of Pb by synthetic chelates has been reported to increase bioavailability, uptake, and translocation of this metal in plants. In this work the potential phytotoxic effects of Pb-EDTA were investigated in Phaseolus vulgaris L. cv. Limburgse vroege plants grown on hydroponics. Addition of 50 microM Pb-EDTA...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1034/j.1399-3054.2002.1150307.x
更新日期:2002-07-01 00:00:00
abstract::In a previous work, it was shown that bicarbonate (one of the most important factors causing Fe chlorosis in Strategy I plants) can limit the expression of several genes involved in Fe acquisition. Hypoxia is considered another important factor causing Fe chlorosis, mainly on calcareous soils. However, to date it is n...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12076
更新日期:2014-01-01 00:00:00
abstract::alpha-Mannosidase (EC 3.2.1.24) from rice dry seeds was purified to homogeneity. Optimum pH and Km for pNP-alpha-Man hydrolysis were pH 4.3-4.5 and 1.04 mM, respectively. The enzyme digested mannobioses such as Manalpha-1,2Man, Manalpha-1,6Man, Manalpha-1,3Man but Manalpha-1,4Man. Zn2+ ion was required for the activit...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1034/j.1399-3054.2001.1120103.x
更新日期:2001-05-01 00:00:00
abstract::EaF82, a gene identified in previous studies of the variegated plant Epipremnum aureum, exhibited a unique expression pattern with greater transcript abundance in yellow sectors than green sectors of variegated leaves, but lower abundance in regenerated pale yellow plants than in green plants derived from leaf tissue ...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12219
更新日期:2014-12-01 00:00:00
abstract::In bread wheat, besides malate, the importance of citrate efflux for Al tolerance has also been reported. For better understanding the Al tolerance mechanism in bread wheat, here, we performed both a molecular characterization of the citrate transporter gene TaMATE1 and an investigation on the upstream variations in c...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12179
更新日期:2014-11-01 00:00:00
abstract::Cuttings of Populus kangdingensis C. Wang et Tung and Populus cathayana Rehder were examined during a single growing season in a greenhouse for comparative analysis of their physiological and proteomic responses to drought stress. The said species originate from high and low altitudes, respectively, of the eastern Him...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2010.01375.x
更新日期:2010-08-01 00:00:00
abstract::Developmental changes of photochemical and non-photochemical processes and the antioxidant system in the shaded peel vs the sun-exposed peel of 'Gala' apple and their responses to sudden exposure of high light were determined to understand the susceptibility of the shaded peel to high light damage with fruit developme...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2008.01131.x
更新日期:2008-10-01 00:00:00
abstract::Global warming is predicted to cause more intense extreme events such as heat waves, flooding and severe droughts, producing significant effects on agriculture. In tropics, climate change will severely impact livestock production affecting water availability, forage quality and food for cattle. We investigated the iso...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12891
更新日期:2019-02-01 00:00:00
abstract::Mathematical models of light attenuation and canopy photosynthesis suggest that crop photosynthesis increases by more uniform vertical irradiance within crops. This would result when a larger proportion of total irradiance is applied within canopies (interlighting) instead of from above (top lighting). These irradianc...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2009.01333.x
更新日期:2010-03-01 00:00:00
abstract::An in situ study of enzymes involved in sucrose to hexose-phosphate conversion during in vitro stolon-to-tuber transition of potato (Solanum tuberosum L. cv. Bintje) was employed to follow developmental changes in spatial patterns. In situ activity of the respective enzymes was visualized by specific activity-staining...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1034/j.1399-3054.2002.1150218.x
更新日期:2002-06-01 00:00:00
abstract::The quinolinate phosphoribosyl transferase (QPT) is a key enzyme that converts quinolinic acid into nicotinic acid mononucleotide. The QPT gene plays an essential role in the pyridine nucleotide cycle as well as in the biosynthetic pathway of the alkaloid nicotine. However, a clear role for QPT is yet to be characteri...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12559
更新日期:2017-07-01 00:00:00
abstract::Peroxiredoxins (Prxs) are ubiquitous thiol-dependent peroxidases capable of eliminating a variety of peroxides through reactive catalytic cysteines, which are regenerated by reducing systems. Based on amino acid sequences and their mode of catalysis, five groups of thiol peroxidases have been distinguished in plants, ...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2008.01097.x
更新日期:2008-07-01 00:00:00
abstract::The ATP-binding cassette (ABC) superfamily is a large protein family with diverse physiological functions in all kingdoms of life. One distinguished subfamily, the pleiotropic drug resistance (PDR) transporters, has only been identified in plants and fungi. Here, we identified a Nicotiana tabacum PDR gene, NtPDR6, whi...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12267
更新日期:2015-02-01 00:00:00
abstract::A novel membrane lipoxygenase (LOX; EC 1.13.11.12) from eggplant (Solanum melongena L. cv. Belleza negra) fruit chloroplasts has been purified 20-fold to a specific activity of 207 enzymatic units per mg of protein with a yield of 72%. The purification was carried out by sonicating the chloroplastic membranes in the p...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1034/j.1399-3054.2001.1110303.x
更新日期:2001-03-01 00:00:00
abstract::In the context of an increased risk of extreme drought events across Europe during the next decades, the capacity of trees to recover and survive drought periods awaits further attention. In summer 2005, 4-year-old beech (Fagus sylvatica L.) saplings were watered regularly or were kept for 4 weeks without irrigation i...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2007.00972.x
更新日期:2007-11-01 00:00:00
abstract::Developing drought-tolerant cultivars is mainly restricted due to poor knowledge of the mechanism behind drought tolerance. In the present work, available germplasm of Vigna radiata (mung bean) was screened for drought tolerance using multiple agronomic and physiological parameters and used to selected one drought-tol...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.13337
更新日期:2021-01-25 00:00:00
abstract::Partial peptide sequence of a 36 kDa protein from common bean embryo axes showed 100% identity with a reported beta-subunit of a heterotrimeric G protein from soybean. Analysis of the full sequence showed 96.6% identity with the reported soybean G(beta)-subunit, 86% with RACK1B and C from Arabidopsis and 66% with huma...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2009.01280.x
更新日期:2009-11-01 00:00:00