Abstract：
:A quantitative PCR method was used to map a functional origin of DNA replication in the hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene in normal human fibroblasts. This PCR method measures the abundance of specific sequences in short fragments of newly replicated DNA from logarithmically growing cells. Quantitative measurements rely on synthetic molecules (competitors) that amplify with the same primer sets as the target molecules, but generate products of different sizes. This method was first utilized to determine the position of the replication origin near the lamin B2 gene (Giacca et al. [1994] Proc. Natl. Acad. Sci. U S A. 91:7119-7123). In the present study, primer sets were tested along a 16-kb region near exon 1 of the HPRT gene. The most abundant fragment was found to be located in the first intron of HPRT, just downstream of the promoter and exon 1 of the gene, and approximately 3.5 kb upstream of a previously reported autonomously replicating sequence (Sykes et al. [1988] Mol. Gen. Genet. 212:301-309).
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Cohen SM,Brylawski BP,Cordeiro-Stone M,Kaufman DGdoi
10.1002/jcb.10136subject
Has Abstractpub_date
2002-01-01 00:00:00pages
346-56issue
2eissn
0730-2312issn
1097-4644pii
10.1002/jcb.10136journal_volume
85pub_type
杂志文章abstract：:lncRNAs are responsible for a variety of diseases, including gastric cancer (GC). Many recent studies have reported that lncRNAs can serve as crucial regulators of various genes. Nevertheless, the biological function of lncRNA damage induced noncoding (DINO) remained poorly investigated in GC. Therefore, in our presen...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28394
更新日期：2019-02-18 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) and angiopoietins regulate endothelial cell survival and migration and are essential for angiogenesis. Considerable progress has been made towards understanding hypoxia-mediated regulation of VEGF and its receptors. In contrast, little is known about the regulation of angiopoi...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.10148
更新日期：2002-01-01 00:00:00
abstract：:"microRNA-383 suppresses the PI3K-AKT-MTOR signaling pathway to inhibit development of cervical cancer via downregulating PARP2," by Peng Teng, Yan Jiao, Min Hao, Xin Tang, J Cell Biochem. 2018; 5243-5252: The above article, published online on 13 December 2017 in Wiley Online Library (https://onlinelibrary.wiley.com/...
journal_title：Journal of cellular biochemistry
pub_type： 撤回出版物
doi：10.1002/jcb.29854
更新日期：2021-01-01 00:00:00
abstract：:Hamster embryo cells metabolize benzo(a)pyrene to derivatives that covalently modify the nuclear macromolecules including proteins. Not all proteins are modified to the same extent nor by the same metabolites. In particular, a protein of apparent molecular weight 32,000 is highly modified by derivatives of trans-9,10-...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.1982.240180411
更新日期：1982-01-01 00:00:00
abstract：:Recent studies have shown that only breast cancer epithelial cells with intact p53 can induce metallothionein (MT) synthesis after exposure to metals. In this study, the potential role of p53 on regulation of MT was investigated. Results demonstrate that zinc and copper increased metal response elements (MREs) activit...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21381
更新日期：2007-12-15 00:00:00
abstract：:The examination of scaffold attachment factor B1 (SAFB1) and its multiple functions and tasks in cellular processes provides insight into its role in diseases, such as cancer. SAFB1 is a large multi-domain protein with well-described functions in transcriptional repression, and RNA splicing. It is ubiquitously express...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22420
更新日期：2010-02-01 00:00:00
abstract：:The obesity is a result of energy imbalance and the increase in thermogenesis seems an interesting alternative for the treatment of this disease. The mechanism of energy expenditure through thermogenesis is tightly articulated in the hypothalamus by leptin. The hypothalamic extracellular signal-regulated kinase-1/2 (E...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27426
更新日期：2019-01-01 00:00:00
abstract：:Arctigenin is a dibenzylbutyrolactone lignan isolated from Bardanae fructus, Arctium lappa L, Saussureamedusa, Torreya nucifera, and Ipomea cairica. It has been reported to exhibit anti-inflammatory activities, which is mainly mediated through its inhibitory effect on nuclear transcription factor-kappaB (NF-κB). But t...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.23198
更新日期：2011-10-01 00:00:00
abstract：:Several independent assay procedures were used to estimate the activities of the enzyme constituents of the phosphoenolpyruvate-sugar phosphotransferase system (PTS) in osmotically shocked bacterial membrane vesicles. The soluble enzymes of the system were found to be in association with the membrane by several criter...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.1982.240180210
更新日期：1982-01-01 00:00:00
abstract：:Scribble (Scrib) is a large multi-domain cytoplasmic protein that was first identified through its requirement for the establishment of epithelial polarity. We tested the hypotheses that Scrib asssociates with the basolateral membrane via multiple domains, binds specific protein partners, and is part of a multimeric c...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.20992
更新日期：2006-10-01 00:00:00
abstract：:Emerging evidence suggests that long noncoding RNAs (lncRNAs) are involved in many biological processes, such as cell growth, differentiation, apoptosis, and autophagy. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), highly expressed in endothelial cells, is well conserved and implicated in endothelia...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27694
更新日期：2019-03-01 00:00:00
abstract：:ERK1 and ERK2 share a great deal of homology and have been presumed to have similar functions. Available antibodies recognize both isoforms making the elucidation of functional differences challenging. Mitogen-activated protein (MAP) kinase networks are commonly depicted in the literature as linear and sequential phos...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.25896
更新日期：2017-08-01 00:00:00
abstract：:Chondrocyte differentiation is a fundamental process during endochondral ossification. Several factors regulate maturation via the activity of downstream signaling pathways that target specific transcription factors and regulate chondrocyte-specific genes. In this study, we investigated the mechanisms involved in the ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.10677
更新日期：2003-12-15 00:00:00
abstract：:Long noncoding RNAs (lncRNAs) areinvolvedin a variety of biological processes. In silkworm, numerous lncRNAs have been predicted through deep transcriptome sequencing, but no functional role has been experimentally validated yet. Here, we characterized a new lncRNA iab-1 that was mainly encoded by the intergenic regio...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28990
更新日期：2019-10-01 00:00:00
abstract：:Ischemia/reperfusion injury is a tissue injury occurring post-reperfusion of tissues with pre-existing ischemia. A good blood supply to tissues aids in the survival of ischemic tissue, however, due to prolonged ischemia the levels of ATP decrease and pH declines leading to acidosis. Reduced ATP leads to an increase in...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.26842
更新日期：2018-09-01 00:00:00
abstract：:Osteosarcoma (OS) is the most malignant primary bone tumor in children and adolescents with limited treatment options and poor prognosis. Recently, aberrant expression of Runx2 has been found in OS, thereby contributing to the development, and progression of OS. However, the upstream signaling molecules that regulate ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.26260
更新日期：2018-01-01 00:00:00
abstract：:p38 mitogen-activated protein kinase (MAPK) is of fundamental importance in a cell's response to environmental stresses, cytokines and DNA damage. p38 resides in the cytoplasm of resting cells, and translocates into the nucleus upon activation, yet the exact mechanisms remain largely unclear. We show here that the pho...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22675
更新日期：2010-08-15 00:00:00
abstract：:Leptin is a protein involved in the regulation of food intake and in the immune and inflammatory responses, among other functions. Evidences demonstrate that obesity is directly associated with high levels of leptin, suggesting that leptin may directly link obesity with the elevated cardiovascular and renal risk assoc...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.25526
更新日期：2016-10-01 00:00:00
abstract：:Chondrosarcoma (CHS) is a common malignant bone sarcoma and its occurrence increases with age. microRNAs (miRNAs) are a class of noncoding RNAs that participate in various biological processes and disease pathogenesis by targeting functional messenger RNA (mRNA). However, the modulation of miRNAs in CHS remains largel...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28241
更新日期：2019-06-01 00:00:00
abstract：:Prostate cancer is the commonest non-skin cancer in men. Incidence and mortality rates of this tumor vary strikingly throughout the world. Although several factors have been implicated to explain this remarkable variation, lifestyle and dietary factors may play a dominant role, with sex hormones behaving as intermedia...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.21529
更新日期：2007-11-01 00:00:00
abstract：:Extracellular matrix (ECM) accumulation plays a key role in the progression of bladder outlet obstruction (BOO). Muscarinic receptors have been widely reported to serve as pivotal regulators in lung tissue remodeling. However, the influence of them on human bladder smooth muscle cells (HBSMCs) and the underlying molec...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.29688
更新日期：2020-11-01 00:00:00
abstract：:The Skp1-Cul1-F-box protein (SCF) E3 ligase complex is one of the largest ubiquitin E3 ligase families. FBXL19, a F-box protein in SCFFBXL19 E3 ligase complex, regulates a variety of cellular responses including cell migration. We have shown that FBXL19 is not stable and its degradation is mediated by the ubiquitin-pr...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.29860
更新日期：2020-10-14 00:00:00
abstract：:Glucocorticoids (GCs) modulate insulin-like growth factor action in cartilage through mechanisms that are complex and insufficiently defined, especially in the context of cranio-facial growth. Because the family of IGF-binding proteins (IGFBP-1 to -6) is important in the regulation of IGF availability and bioactivity,...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.10396
更新日期：2003-04-01 00:00:00
abstract：:Diabetes mellitus (DM), one of the most prevalent metabolic diseases in the world population, is associated with a number of comorbid conditions including obesity, pancreatic endocrine changes, and renal and cardio-cerebrovascular alterations, coupled with peripheral neuropathy and neurodegenerative disease, some of t...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28094
更新日期：2019-09-01 00:00:00
abstract：:Neuropathic pain (NP) is a substantial clinical problem causing great injury to people word-widely. Although gene expression analyses had been performed previously, the mechanisms underlying the etiology and development of NP are still poorly understood. To understand the function genes involved in the etiology and de...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.26098
更新日期：2017-12-01 00:00:00
abstract：:Intact embryonic chick neural retina cells have at their surface an N-acetylgalactosaminyltransferase which catalyzes the incorporation of N-acetylgalactosamine from UDP-N-acetylgalactosamine into endogenous macromolecular acceptors. The enzyme along with its endogenous acceptors can be isolated as a particulate compl...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240320205
更新日期：1986-01-01 00:00:00
abstract：:It is known that the MyoD family members (MyoD, Myf5, myogenin, and MRF4) play a pivotal role in the complex mechanism of skeletal muscle cell differentiation. However, fragmentary information on transcription factor-specific regulation is available and data on their post-transcriptional and post-translational behavio...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22360
更新日期：2009-12-15 00:00:00
abstract：:Ligamentum flavum (LF)-derived mesenchymal stem cells (MSCs) have been implicated in the pathogenesis of calcification of the ligamentum flavum (CLF) leading to the increased presence of chondrocyte-like cells and calcium deposition in CLF; however, the mechanisms of LF-MSCs in differentiation are not defined. In this...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27611
更新日期：2019-03-01 00:00:00
abstract：:Complete resection of malignant glioblastomas is usually impossible because of diffuse and widespread invasion of tumor cells, and complementary approaches need to be developed in order to improve the efficacy of current treatments. Consumption of fruits and berries has been associated with decreased risk of developin...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21023
更新日期：2007-01-01 00:00:00
abstract：:In response to tissue injury connective tissue synthesis occurs either normally or abnormally, which is mediated by transforming growth factor-beta (TGF-beta) and other growth factors. This article will be primarily concerned with the response of injured tissues at the gene level of Type I procollagen synthesis in res...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.10599
更新日期：2003-09-01 00:00:00
