Abstract:
:The ability of DNA screening techniques such as Temperature Gradient Gel Electrophoresis (TGGE) and Heteroduplex Analysis to provide resolution approaching that provided by DNA sequencing for a fraction of the time, effort and expense point to them as the logical successor to allozyme electrophoresis for population genetics. Here we present a novel alternative to the standard TGGE/Heteroduplex Analysis protocol - Outgroup Heteroduplex Analysis (OHA). We assess this technique's sensitivity in comparison to previous screening approaches using a known hierarchy of sequence differences. Our data show that Outgroup Heteroduplex Analysis has greatly increased sensitivity for screening DNA variants from that of TGGE used alone and is easily applicable to large numbers of samples. Using this technique we can consistently detect differences of as small as one base change in a 433-base-pair fragment of Control Region mitochondrial DNA from Melomys cervinipes (an Australian rodent). The approach should easily be extendable to nuclear loci and is not necessarily dependent on the use of a denaturing gradient. When combined with a targeted sequencing effort, OHA provides a sensitive and simple means of obtaining allele/haplotype frequencies and their phylogenies for population and phylogeographic studies in molecular ecology.
journal_name
Mol Ecoljournal_title
Molecular ecologyauthors
Campbell NJ,Harriss FC,Elphinstone MS,Baverstock PRdoi
10.1111/j.1365-294x.1995.tb00234.xsubject
Has Abstractpub_date
1995-08-01 00:00:00pages
407-18issue
4eissn
0962-1083issn
1365-294Xjournal_volume
4pub_type
杂志文章abstract::The declining health of coral reefs worldwide is likely to intensify in response to continued anthropogenic disturbance from coastal development, pollution, and climate change. In response to these stresses, reef-building corals may exhibit bleaching, which marks the breakdown in symbiosis between coral and zooxanthel...
journal_title:Molecular ecology
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1111/j.1365-294X.2008.03962.x
更新日期:2008-11-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1111/mec.13499
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pub_type: 杂志文章
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更新日期:2000-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/mec.14143
更新日期:2017-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/mec.13696
更新日期:2016-07-01 00:00:00
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journal_title:Molecular ecology
pub_type: 杂志文章
doi:10.1111/j.1365-294X.2010.04811.x
更新日期:2010-10-01 00:00:00
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pub_type: 评论,杂志文章
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pub_type: 杂志文章
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journal_title:Molecular ecology
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abstract::The success of coral reefs is due to obligate mutualistic symbioses involving invertebrates and photosynthetic dinoflagellate symbionts belonging to the genus Symbiodinium. In the Caribbean, the vast majority of octocorals and other invertebrate hosts associate with Symbiodinium clade B, and more selectively, with a s...
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journal_title:Molecular ecology
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00
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journal_title:Molecular ecology
pub_type: 杂志文章
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journal_title:Molecular ecology
pub_type: 杂志文章
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