Expression of the Hevea brasiliensis (H.B.K.) Mull. Arg. 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase 1 in Tobacco Results in Sterol Overproduction.

abstract：:Calcium affects the stability of cortical microtubules (MTs) in lysed protoplasts. This calmodulin (CaM)-mediated interaction may provide a mechanism that serves to integrate cellular behavior with MT function. To test the hypothesis that CaM associates with these MTs, monoclonal antibodies were produced against CaM, ...

journal_title：Plant physiology

authors： Fisher DD,Cyr RJ

更新日期：1993-10-01 00:00:00

abstract：:Free protoplasts were prepared from the living bark tissue of the trunk of summer and winter black locust trees by enzymic digestion of thin slices of the tissue for 3 hours in a medium containing 2% Onozuka cellulase, 2% Rhozyme pectinase, and 2% Driselase in mannitol solutions using 0.4 molar mannitol for summer tis...

journal_title：Plant physiology

authors： Siminovitch D

更新日期：1979-04-01 00:00:00

abstract：:Hydroperoxide lyase (HPLS) activity in soybean (Glycine max) seed/seedlings, leaves, and chloroplasts of leaves required detergent solubilization for maximum in vitro activity. On a per milligram of protein basis, more HPLS activity was found in leaves, especially chloroplasts, than in seeds or seedlings. The total yi...

journal_title：Plant physiology

authors： Gardner HW,Weisleder D,Plattner RD

更新日期：1991-11-01 00:00:00

abstract：:Plant growth and development are strongly dependent on sink-source interactions. In the majority of plants, sucrose (Suc) is the dominant form in which photo-assimilate is transported from source to sinks. Although the effects of Suc on photosynthetic metabolism have been intensively studied, the effect of Suc supply ...

journal_title：Plant physiology

authors： Roessner-Tunali U,Urbanczyk-Wochniak E,Czechowski T,Kolbe A,Willmitzer L,Fernie AR

更新日期：2003-10-01 00:00:00

abstract：:Stomatal movements depend on an osmoregulation process in which swelling or shrinking of the guard cells opens or closes the stomatal pore. Ions and water fluxes are an essential aspect of guard cell osmoregulation. Thus far, studies of these fluxes have focused on the guard cell plasma membrane. Guard cells, however,...

journal_title：Plant physiology

authors： Amodeo G,Escobar A,Zeiger E

更新日期：1994-07-01 00:00:00

abstract：:Fusicoccin inhibits the germination of pea (Pisum sativum L. cv Progress 9) seeds by decreasing the growth of the embryonal axis and by stimulating the fresh weight increase of the cotyledons. The growth of isolated embryonal axes in the presence of sucrose and KCl is stimulated by fusicoccin. The effect of fusicoccin...

journal_title：Plant physiology

authors： Lioi L,Petruzzelli L,Morgutti S,Cocucci SM

更新日期：1984-11-01 00:00:00

abstract：:Light-mediated seedling development is coordinately controlled by a variety of key regulators. Here, we identified two B-box (BBX)-containing proteins, BBX30 and BBX31, as repressors of photomorphogenesis. ELONGATED HYPOCOTYL5, a central regulator of light signaling, directly binds to the G-box cis-element present in ...

journal_title：Plant physiology

authors： Heng Y,Lin F,Jiang Y,Ding M,Yan T,Lan H,Zhou H,Zhao X,Xu D,Deng XW

更新日期：2019-05-01 00:00:00

abstract：:The red light requirement for positive gravitropism in roots of corn (Zea mays cv "Merit") provides an entry for examining the participation of calcium in gravitropism. Applications of calcium chelators inhibit the light response. Calcium channel blockers (verapamil, lanthanum) can also inhibit the light response, a...

journal_title：Plant physiology

authors： Perdue DO,LaFavre AK,Leopold AC

更新日期：1988-01-01 00:00:00

abstract：:The role of H(+) excretion in auxin-induced growth of soybean hypocotyl tissues has been investigated, using tissues whose cuticle was rendered permeable to protons or buffers by scarification (scrubbing). Indoleacetic acid induces both elongation and H(+) excretion after a lag of 10 to 12 minutes. Cycloheximide inhib...

journal_title：Plant physiology

authors： Rayle DL,Cleland RE

更新日期：1980-09-01 00:00:00

abstract：:We have previously identified GAMYB, a gibberellin (GA)-regulated transcriptional activator of alpha-amylase gene expression, in aleurone cells of barley (Hordeum vulgare). To examine the regulation of GAMYB expression, we describe the use of nuclear run-on experiments to show that GA causes a 2-fold increase in the r...

journal_title：Plant physiology

authors： Gubler F,Chandler PM,White RG,Llewellyn DJ,Jacobsen JV

更新日期：2002-05-01 00:00:00

abstract：:Intact spinach and barley chloroplast normally incorporate (14)C-acetate into palmitate and oleate as the major (14)C fatty acids. Addition of nitrite markedly altered the relative patterns of the products with the appearance of stearate, a drop in oleate, but no marked change in palmitate. Arsenite greatly increased ...

journal_title：Plant physiology

authors： Kannangara CG,Stumpf PK

更新日期：1972-04-01 00:00:00

abstract：:Pea seeds (Pisum sativum L. ;Alaska') with intact seedcoats (WC) and with seedcoats removed (WOC) were soaked in distilled water for 24 hours at 20 degrees . The water, containing the pea diffusate, was decanted after the second, fourth, sixth, eighth, twelfth, and twenty-fourth hour and analyzed for total nitrogen, a...

journal_title：Plant physiology

authors： Larson LA

更新日期：1968-02-01 00:00:00

abstract：:Spectral scanning was used to provide estimates of the leakage of the cyanogenic glucoside, dhurrin (p-hydroxy-[S]-mandelonitrile-beta-d-glucoside), and its metabolite, p-hydroxybenzaldehyde (p-HB), from young light-grown shoots of Atlas sorghum (Sorghum bicolor [L.] Moench) when these shoots were immersed in water, t...

journal_title：Plant physiology

authors： Haskins FA,Gorz HJ

更新日期：1984-07-01 00:00:00

abstract：:Photosynthesis, primary productivity, N content, and N(2) fixation were determined as a function of applied NH(4) (+) in peas (Pisum sativum L. cv. Alaska) which were inoculated or not inoculated with Rhizobium leguminosarum. Cabon dioxide exchange rate (CER) increased 10-fold, total N content 7-fold, and total dry we...

journal_title：Plant physiology

authors： Bethlenfalvay GJ,Abu-Shakra SS,Phillips DA

更新日期：1978-07-01 00:00:00

abstract：:Changes in the levels of the four subunits of the mitochondrial enzyme glycine decarboxylase (EC 2.1.2.10) have been investigated during development in the 8 day old primary leaf of wheat (Triticum aestivum L.). Proteins were extracted from wheat leaf sections between the basal meristem and 8.5 centimeters. The indivi...

journal_title：Plant physiology

authors： Rogers WJ,Jordan BR,Rawsthorne S,Tobin AK

更新日期：1991-07-01 00:00:00

abstract：:The NB mitochondrial genome found in most fertile varieties of commercial maize (Zea mays subsp. mays) was sequenced. The 569,630-bp genome maps as a circle containing 58 identified genes encoding 33 known proteins, 3 ribosomal RNAs, and 21 tRNAs that recognize 14 amino acids. Among the 22 group II introns identified,...

journal_title：Plant physiology

authors： Clifton SW,Minx P,Fauron CM,Gibson M,Allen JO,Sun H,Thompson M,Barbazuk WB,Kanuganti S,Tayloe C,Meyer L,Wilson RK,Newton KJ

更新日期：2004-11-01 00:00:00

abstract：:Prolonged growth of cell cultures of sycamore (Acer pseudoplatanus L.) on agar medium containing myo-inositol-2-(3)H resulted in incorporation of label predominately into uronosyl and pentosyl units of cell wall polysaccharides. Procedures normally used to distinguish between pectic substance and hemicellulose yielded...

journal_title：Plant physiology

authors： Roberts RM,Loewus F

更新日期：1966-11-01 00:00:00

abstract：:The accumulation and reduction of nitrate in the presence of the nitrogen metabolites asparagine (Asn) and glutamine (Gln) and the carbon metabolite sucrose (Suc) were examined in maize (Zea mays L.) seedlings in an attempt to separate their effects on the nitrate uptake system and the nitrate reduction system. After ...

journal_title：Plant physiology

authors： Sivasankar S,Rothstein S,Oaks A

更新日期：1997-06-01 00:00:00

abstract：:High-throughput genotype screening is rapidly becoming a standard research tool in the post-genomic era. A major bottleneck currently exists, however, that limits the utility of this approach in the plant sciences. The rate-limiting step in current high-throughput pipelines is that tissue samples from living plants mu...

journal_title：Plant physiology

authors： Krysan P

更新日期：2004-07-01 00:00:00

abstract：:The accumulation of delta-aminolevulinic acid and activities of delta-aminolevulinic acid dehydrase were examined in the marine diatom, Skeletonema costatum, grown in the presence of levulinic acid. Levulinic acid concentrations greater than 10 mm affect growth and morphology, and inhibit chlorophyll synthesis. The al...

journal_title：Plant physiology

authors： Owens TG,Riper DM,Falkowski PG

更新日期：1978-10-01 00:00:00

abstract：:Red pines (Pinus resinosa Ait.) were grown in a pasteurized sandy loam either unamended with phosphate or fertilized with one of two levels of phosphate (34 or 136 mg/kg) as superphosphate, and with and without addition of Hebeloma arenosa inoculum. Shoot and total dry weights of mycorrhizal seedlings grown in soil un...

journal_title：Plant physiology

authors： Macfall JS,Slack SA,Wehrli S

更新日期：1992-10-01 00:00:00

abstract：:A proteinase was purified from germinated barley (green malt from Hordeum vulgare L. cv Morex) by acidic extraction, ammonium sulfate fractionation and successive chromatographies on CM-cellulose, hemoglobin sepharose, Sephadex G-75 and organomercurial agarose columns. The overall purification and final recovery were ...

journal_title：Plant physiology

authors： Poulle M,Jones BL

更新日期：1988-12-01 00:00:00

abstract：:The Arabidopsis (Arabidopsis thaliana) sucrose transporter AtSUC1 (At1g71880) is highly expressed in pollen; however, its function has remained unknown. Here, we show that suc1 mutant pollen is defective in vivo, as evidenced by segregation distortion, and also has low rates of germination in vitro. AtSUC1-green fluor...

journal_title：Plant physiology

authors： Sivitz AB,Reinders A,Ward JM

更新日期：2008-05-01 00:00:00

abstract：:Under normal field growth conditions, canola (Brassica napus) seeds produce chloroplasts during early seed development and then catabolize the photosynthetic machinery during seed maturation, producing mature seeds at harvest that are essentially free of chlorophyll (Chl). However, frost exposure early in canola seed ...

journal_title：Plant physiology

authors： Chung DW,Pruzinská A,Hörtensteiner S,Ort DR

更新日期：2006-09-01 00:00:00

abstract：:Traditionally, phenotype-driven forward genetic plant mutant studies have been among the most successful approaches to revealing the roles of genes and their products and elucidating biochemical, developmental, and signaling pathways. A limitation is that it is time consuming, and sometimes technically challenging, to...

journal_title：Plant physiology

authors： Ajjawi I,Lu Y,Savage LJ,Bell SM,Last RL

更新日期：2010-02-01 00:00:00

abstract：:Autoradiographs of (22)Na-loaded bean (Phaseolus vulgaris L. cv. ;Brittle Wax') leaf slices showed that most of the tracer was concentrated in vascular tissue. Rubidium-86 was uniformly distributed in slices that had been incubated in darkness; after incubation in the light many small dark spots appeared on the autora...

journal_title：Plant physiology

authors： Jacoby B

更新日期：1975-06-01 00:00:00

abstract：:The use of only the upper part of the hypocotyl and the cotyledons in the Amaranthus tricolor bioassay for cytokinins, instead of the whole seedling, was found to reduce the endogenous response and give a higher benzyladenine-dependent response. There is no marked difference in the uptake or metabolism of benzyladenin...

journal_title：Plant physiology

authors： Elliott DC

更新日期：1979-02-01 00:00:00

abstract：:The concentration-dependent stimulatory and inhibitory effect of N-glycans on tomato (Lycopersicon esculentum Mill.) fruit ripening was recently reported (B. Priem and K.C. Gross [1992] Plant Physiol 98: 399-401). We report here the structure of 10 free N-glycans in mature green tomatoes. N-Glycans were purified from ...

journal_title：Plant physiology

authors： Priem B,Gitti R,Bush CA,Gross KC

更新日期：1993-06-01 00:00:00

abstract：:Tonoplast vesicles were purified from a microsomal fraction isolated from etiolated soybean hypocotyls (Glycine max L.) by preparative free-flow electrophoresis. Marker enzyme determinations and immunoblot analysis against the vacuolar-ATPase confirmed the nature and the purity of the isolated membranes. A purified to...

journal_title：Plant physiology

authors： Canut H,Brightman A,Boudet AM,Morré DJ

更新日期：1990-11-01 00:00:00

abstract：:A radioimmunoassay for glyceollin I, the major phytoalexin produced by soybean (Glycine max [L.] Merr.), has been developed. Antibodies were raised in rabbits against a glyceollin I-bovine serum albumin conjugate. The antisera were used to establish a radioimmunoassay for glyceollin I using [(125)I]glyceollin I as the...

journal_title：Plant physiology

authors： Moesta P,Hahn MG,Grisebach H

更新日期：1983-10-01 00:00:00