Restriction endonuclease analysis of plasmid DNA from methicillin-resistant Staphylococcus aureus: clinical application over a three-year period.

Abstract:

OBJECTIVE:To investigate trends in plasmid types of methicillin-resistant Staphylococcus aureus over a 3-year period and determine the clinical impact of plasmid typing. DESIGN:Restriction endonuclease analysis of plasmid DNA (REAP) was used to type 270 clinical isolates. SETTING:A Veterans Affairs Medical Center. RESULTS:Forty-four different REAP types were identified and, of these, 23 appeared only once. Types designed as V1 and V2 were more commonly found in nosocomial cases than in community-acquired cases (p < .05); whereas the K and D types were more common in isolates from community-acquired cases (p < .05). In 30 (11%) isolates, no plasmid was detected. When combined with epidemiological data, REAP typing revealed 4 small outbreaks that would have been missed using traditional epidemiological methods alone. In large outbreaks involving 10 or more cases, REAP typing data revealed unsuspected patterns of transmission. CONCLUSIONS:Multiple plasmid types were present in this endemic setting. Restriction endonuclease analysis of plasmid DNA was a practical and valuable adjunct to traditional epidemiological methods.

authors

Trilla A,Nettleman MD,Hollis RJ,Fredrickson M,Wenzel RP,Pfaller MA

doi

10.1086/646627

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

29-35

issue

1

eissn

0899-823X

issn

1559-6834

journal_volume

14

pub_type

杂志文章
  • Indirect Versus Direct Standardization Methods for Reporting Healthcare-Associated Infections: An Analysis of Central Line-Associated Bloodstream Infections in Maryland.

    abstract::Whether healthcare-associated infection data should be presented using indirect (current CMS/CDC methodology) or direct standardization remains controversial. We applied both methods to central-line-associated bloodstream infection data from 45 acute-care hospitals in Maryland from 2012 to 2014. We found that the 2 me...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1017/ice.2017.120

    authors: O'Hara LM,Masnick M,Leekha S,Jackson SS,Blanco N,Harris AD

    更新日期:2017-08-01 00:00:00

  • Transmission of hepatitis C in a pharmacologic study.

    abstract:OBJECTIVE:To describe an outbreak of hepatitis C in a clinical research study. DESIGN:Observational study. SETTING:Tertiary-care hospital. PATIENTS:Healthcare workers who volunteered to be subjects in a study of the metabolic effects of inhaled and oral corticosteroids who were unwittingly exposed to hepatitis C vir...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/501848

    authors: Saginur R,Nixon J,Devries B,Bruce N,Carruthers C,Scully L,Berger R,Leech J,Nicolle L,Mackenzie A

    更新日期:2001-11-01 00:00:00

  • Quality assessment of hospital discharge database for routine surveillance of hip and knee arthroplasty-related infections.

    abstract:OBJECTIVE:Surgical site infection (SSI) surveillance represents a key method of nosocomial infection control programs worldwide. However, most SSI surveillance systems are considered to be poorly cost effective regarding human and economic resources required for data collection and patient follow up. This study aims to...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/676423

    authors: Grammatico-Guillon L,Baron S,Gaborit C,Rusch E,Astagneau P

    更新日期:2014-06-01 00:00:00

  • National Survey of Environmental Cleaning and Disinfection in Hospitals in Thailand.

    abstract::More than 90% of Thai hospitals surveyed reported implementing environmental cleaning and disinfection (ECD) protocols. Hospital epidemiologist presence was associated with the existence of an ECD checklist (P=.01) and of ECD auditing (P=.001), while good and excellent hospital administrative support were associated w...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1017/ice.2017.171

    authors: Apisarnthanarak A,Weber DJ,Ratz D,Saint S,Khawcharoenporn T,Greene MT

    更新日期:2017-10-01 00:00:00

  • The use of best practice alerts with the development of an antimicrobial stewardship navigator to promote antibiotic de-escalation in the electronic medical record.

    abstract:OBJECTIVE:Develop a clinical decision support tool comprised of an electronic medical record alert and antimicrobial stewardship navigator to facilitate antimicrobial stewardship. DESIGN:We analyzed alerts targeting antimicrobial de-escalation to assess the effectiveness of the navigator as a stewardship tool. The ale...

    journal_title:Infection control and hospital epidemiology

    pub_type: 临床试验,杂志文章

    doi:10.1086/673977

    authors: Schulz L,Osterby K,Fox B

    更新日期:2013-12-01 00:00:00

  • Burden of healthcare-associated infections at six tertiary-care hospitals in Saudi Arabia: A point prevalence survey.

    abstract::A point prevalence survey was conducted on May 11, 2017, among inpatients at 6 hospitals in Saudi Arabia. The overall point prevalence was 6.8% (114 of 1,666). The most common types of infections were pneumonia (27.2%), urinary tract infections (20.2%), and bloodstream infections (10.5%). Approximately 19.2% of health...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1017/ice.2018.338

    authors: Alshamrani MM,El-Saed A,Alsaedi A,El Gammal A,Al Nasser W,Nazeer S,Balkhy HH

    更新日期:2019-03-01 00:00:00

  • Malaria: protection of the international traveler.

    abstract::Thorough counseling with emphasis on the importance of mosquito bite avoidance, as well as on compliance with prophylactic antimalarial regimens, remains an important task for the physician preparing a patient for international travel. Chloroquine continues to be the drug of choice for prophylaxis in areas not reporti...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章,评审

    doi:10.1086/646703

    authors: Rangel-Frausto MS,Edmond MB

    更新日期:1993-03-01 00:00:00

  • Issues in the management of resistant bacteria in long-term-care facilities.

    abstract::The prevalence of antibiotic-resistant bacteria in the long-term-care setting and the risk to nursing home residents is still unknown. Few studies have been done in community-based nursing homes, and most have focused on colonization rather than infection rates. Concerns about methicillin-resistant Staphylococcus aure...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章,评审

    doi:10.1086/501637

    authors: Bradley SF

    更新日期:1999-05-01 00:00:00

  • Routes and sources of Staphylococcus aureus transmitted to the surgical wound during cardiothoracic surgery: possibility of preventing wound contamination by use of special scrub suits.

    abstract:OBJECTIVES:To trace the routes of transmission and sources of Staphylococcus aureus found in the surgical wound during cardiothoracic surgery and to investigate the possibility of reducing wound contamination, with regard to total counts of bacteria and S. aureus, by wearing special scrub suits. METHODS:A total of 65 ...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/501910

    authors: Tammelin A,Hambraeus A,Ståhle E

    更新日期:2001-06-01 00:00:00

  • Subsequent carbapenem-resistant Enterobacteriaceae (CRE)-associated infections among hospitalized patients with CRE colonization: Impact of antibiotic use and other factors.

    abstract::We demonstrated a 20% rate of subsequent carbapenem-resistant Enterobacteriaceae (CRE)-associated infections among hospitalized CRE carriers. Independent factors associated with the infections were number of colonization sites, central-line insertion, and receiving vancomycin before colonization. These findings unders...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1017/ice.2020.220

    authors: Khawcharoenporn T,Laichuthai W

    更新日期:2020-09-01 00:00:00

  • Evaluation of infection control measures in preventing the nosocomial transmission of multidrug-resistant Mycobacterium tuberculosis in a New York City hospital.

    abstract:OBJECTIVE:To evaluate the efficacy of Centers for Disease Control and Prevention (CDC)-recommended infection control measures implemented in response to an outbreak of multidrug-resistant (MDR) tuberculosis (TB). DESIGN:Retrospective cohort studies of acquired immunodeficiency syndrome (AIDS) patients and healthcare w...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/647075

    authors: Stroud LA,Tokars JI,Grieco MH,Crawford JT,Culver DH,Edlin BR,Sordillo EM,Woodley CL,Gilligan ME,Schneider N

    更新日期:1995-03-01 00:00:00

  • Lack of Comprehensive Outbreak Detection in Hospitals.

    abstract::Timely identification of outbreaks of hospital-associated infections is needed to implement control measures and minimize impact. Survey results from 33 hospitals indicated that most hospitals lacked a formal cluster definition and all targeted a very limited group of prespecified pathogens. Standardized, statisticall...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章,多中心研究

    doi:10.1017/ice.2015.325

    authors: Baker MA,Huang SS,Letourneau AR,Kaganov RE,Peeples JR,Drees M,Platt R,Yokoe DS

    更新日期:2016-04-01 00:00:00

  • Active surveillance for methicillin-resistant Staphylococcus aureus in the neonatal intensive care unit.

    abstract:BACKGROUND:We describe our experience using a real-time polymerase chain reaction (PCR) assay for methicillin-resistant Staphylococcus aureus (MRSA) during a period of active surveillance in the neonatal intensive care unit (NICU) from March 2007 until November 2007. OBJECTIVE:To compare PCR with bacterial culture met...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/605321

    authors: Sarda V,Molloy A,Kadkol S,Janda WM,Hershow R,McGuinn M

    更新日期:2009-09-01 00:00:00

  • Investigations of intrinsic Pseudomonas cepacia contamination in commercially manufactured povidone-iodine.

    abstract:OBJECTIVE:Laboratory investigations were initiated with a povidone-iodine antiseptic solution that was intrinsically contaminated with Pseudomonas cepacia. These investigations were helpful in understanding the microbicidal and chemical properties of iodophor solutions and the mechanism by which P cepacia can survive i...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/646342

    authors: Anderson RL,Vess RW,Carr JH,Bond WW,Panlilio AL,Favero MS

    更新日期:1991-05-01 00:00:00

  • Risk factors for and clinical outcomes of bloodstream infections caused by extended-spectrum beta-lactamase-producing Klebsiella pneumoniae.

    abstract:OBJECTIVE:To evaluate risk factors and treatment outcomes of bloodstream infections caused by extended-spectrum beta-lactamase-producing Klebsiella pneumoniae (ESBL-KP). DESIGN:Retrospective case-control study. Stored blood isolates of K. pneumoniae were tested for ESBL production by NCCLS guidelines, double-disk syne...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/502310

    authors: Kang CI,Kim SH,Kim DM,Park WB,Lee KD,Kim HB,Oh MD,Kim EC,Choe KW

    更新日期:2004-10-01 00:00:00

  • Surgical site infection after cardiac surgery: a simplified surveillance method.

    abstract::We report the results of a 2-year, 7-center program of surveillance of deep sternal wound infection (DSWI) after cardiac surgery. DSWI was defined as the need for reoperation. Stratification data were abstracted from computerized files. The incidence of DSWI was 2.2% (198 of 8,816 cardiac surgery procedures). The risk...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/509853

    authors: Lucet JC,Parisian Mediastinitis Study Group.

    更新日期:2006-12-01 00:00:00

  • Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae: a threat from the kitchen.

    abstract::Food is an established source of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae. Hand hygiene and cooking prevent transmission, but hands could be recontaminated by touching used cutting boards. ESBL-producing Escherichia coli were identified on 12% of cutting boards and 50% of gloves after poultry ...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/675831

    authors: Tschudin-Sutter S,Frei R,Stephan R,Hächler H,Nogarth D,Widmer AF

    更新日期:2014-05-01 00:00:00

  • An outbreak of Pseudomonas aeruginosa respiratory tract infections associated with intrinsically contaminated ultrasound transmission gel.

    abstract::We describe an outbreak of Pseudomonas aeruginosa respiratory tract infections related to intrinsically contaminated ultrasound gel used for intraoperative transesophageal echocardiograms in cardiovascular surgery patients. This investigation led to a product safety alert by the Food and Drug Administration and the de...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/671268

    authors: Chittick P,Russo V,Sims M,Robinson-Dunn B,Oleszkowicz S,Sawarynski K,Powell K,Makin J,Darnell E,Boura JA,Boyanton B,Band J

    更新日期:2013-08-01 00:00:00

  • Prevalence and risk factors associated with vancomycin-resistant Staphylococcus aureus precursor organism colonization among patients with chronic lower-extremity wounds in Southeastern Michigan.

    abstract:BACKGROUND:Of the 13 US vancomycin-resistant Staphylococcus aureus (VRSA) cases, 8 were identified in southeastern Michigan, primarily in patients with chronic lower-extremity wounds. VRSA infections develop when the vanA gene from vancomycin-resistant enterococcus (VRE) transfers to S. aureus. Inc18-like plasmids in V...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/671735

    authors: Tosh PK,Agolory S,Strong BL,Verlee K,Finks J,Hayakawa K,Chopra T,Kaye KS,Gilpin N,Carpenter CF,Haque NZ,Lamarato LE,Zervos MJ,Albrecht VS,McAllister SK,Limbago B,Maccannell DR,McDougal LK,Kallen AJ,Guh AY

    更新日期:2013-09-01 00:00:00

  • Long-term outcomes of an antimicrobial stewardship program implemented in a hospital with low baseline antibiotic use.

    abstract:OBJECTIVE:To evaluate the long-term outcomes of an antimicrobial stewardship program (ASP) implemented in a hospital with low baseline antibiotic use. DESIGN:Quasi-experimental, interrupted time-series study. SETTING:Public safety net hospital with 525 beds. INTERVENTION:Implementation of a formal ASP in July 2008. ...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1017/ice.2015.41

    authors: Jenkins TC,Knepper BC,Shihadeh K,Haas MK,Sabel AL,Steele AW,Wilson ML,Price CS,Burman WJ,Mehler PS

    更新日期:2015-06-01 00:00:00

  • Studies of ventilation efficiency in a protective isolation room by the use of a scale model.

    abstract:OBJECTIVE:To assess the ability of a protective isolation room ventilation system to reduce patient exposure to airborne infectious agents, using a small-scale model that permits cost-effective and unobtrusive study of relevant indices of performance. DESIGN:A one-half scale model of a protective isolation room at the...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/647181

    authors: Marshall JW,Vincent JH,Kuehn TH,Brosseau LM

    更新日期:1996-01-01 00:00:00

  • Changes in healthcare-associated Staphylococcus aureus bloodstream infections after the introduction of a national hand hygiene initiative.

    abstract:BACKGROUND:Interventions that prevent healthcare-associated infection should lead to fewer deaths and shorter hospital stays. Cleaning hands (with soap or alcohol) is an effective way to prevent the transmission of organisms, but rates of compliance with hand hygiene are sometimes disappointingly low. The National Hand...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/677160

    authors: Barnett AG,Page K,Campbell M,Brain D,Martin E,Rashleigh-Rolls R,Halton K,Hall L,Jimmieson N,White K,Paterson D,Graves N

    更新日期:2014-08-01 00:00:00

  • Delays in the application of outbreak control prophylaxis for influenza A in a nursing home.

    abstract:OBJECTIVE:To identify delayed prophylaxis from a pre-existing database and strategies to improve performance. SETTING:A skilled nursing facility with 14 floors (4 buildings). The "outbreak unit" was a 50- to 60-bed floor. METHODS:We performed surveillance during six seasons using one protocol. Prophylaxis was started...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/501978

    authors: Drinka PJ,Krause P,Nest L,Gravenstein S,Goodman B,Shult P

    更新日期:2002-10-01 00:00:00

  • Ventilator-associated tracheobronchitis increases the length of intensive care unit stay.

    abstract:OBJECTIVE:To investigate prospectively the clinical course and risk factors for ventilator-associated tracheobronchitis (VAT) and the impact of VAT on intensive care unit (ICU) morbidity and mortality. DESIGN:Prospective cohort study. SETTING:University Hospital Larissa, Larissa, Greece. PATIENTS:Critical care patie...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/671274

    authors: Karvouniaris M,Makris D,Manoulakas E,Zygoulis P,Mantzarlis K,Triantaris A,Chatzi M,Zakynthinos E

    更新日期:2013-08-01 00:00:00

  • Outbreak of surgical wound infections associated with total hip arthroplasty.

    abstract:OBJECTIVES:Describe an outbreak of surgical wound infections associated with total hip arthroplasty; identify risk factors for surgical wound infection during the pre-outbreak and outbreak periods. SETTING:A 100-bed hospital. From May 1 to September 30, 1988, 7 of 15 patients who underwent total hip arthroplasty devel...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/646592

    authors: Beck-Sague CM,Chong WH,Roy C,Anderson R,Jarvis WR

    更新日期:1992-09-01 00:00:00

  • Is the efficacy of antibiotic prophylaxis for surgical procedures decreasing? Systematic review and meta-analysis of randomized control trials.

    abstract:OBJECTIVE:Rising antibiotic resistance could reduce the effectiveness of antibiotics in preventing postoperative infections. We investigated trends in the efficacy of antibiotic prophylaxis regimens for 3 commonly performed surgical procedures-appendectomy, cesarean section, and colorectal surgery-and 1 invasive diagno...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章,meta分析

    doi:10.1017/ice.2018.295

    authors: Gandra S,Trett A,Alvarez-Uria G,Solomkin JS,Laxminarayan R

    更新日期:2019-02-01 00:00:00

  • Effect of prospective audit and feedback on inpatient fluoroquinolone use and appropriateness of prescribing.

    abstract::We report the effect of prospective audit and feedback (PAF) on inpatient fluoroquinolone (FQN) prescriptions. During the PAF period, FQN use decreased from 39.19 to 29.58 days of therapy per 1,000 patient days (P < .001) and appropriateness improved from 68% to 88% (P < .001). High-yield indications to target include...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1017/ice.2020.339

    authors: Marek CM,Zurek KJ,Degenhardt O,Remtulla S,Teale AS,Cervera C,Hoang HL

    更新日期:2020-12-01 00:00:00

  • The epidemiology of fecal carriage of vancomycin-resistant enterococci.

    abstract::An outbreak of vancomycin-resistant enterococci (VRE) began at the University of Massachusetts Medical Center in May 1993. As of September 1995, we had a total of 253 patients infected or colonized with VRE, with consequent increasing demand for private rooms. We analyzed results of surveillance cultures for VRE of 49...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章,评审

    doi:

    authors: Lai KK,Fontecchio SA,Kelley AL,Melvin ZS,Baker S

    更新日期:1997-11-01 00:00:00

  • Hepatitis B: perceptions, knowledge and vaccine acceptance among registered nurses in high-risk occupations in a university hospital.

    abstract::We employed a questionnaire to survey 334 registered nurses regarding their knowledge, perceptions and vaccine acceptance for Hepatitis B virus (HBV) infections. The study population was those persons working in areas considered at high-risk for contracting HBV. The nurses were asked to provide information regarding b...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/646135

    authors: Spence MR,Dash GP

    更新日期:1990-03-01 00:00:00

  • Efficient recovery of fluoroquinolone-susceptible and fluoroquinolone-resistant Escherichia coli strains from frozen samples.

    abstract::We assessed the rate of recovery of fluoroquinolone-resistant and fluoroquinolone-susceptible Escherichia coli isolates from culture of frozen perirectal swab samples compared with the results for culture of the same specimen before freezing. Recovery rates for these 2 classes of E. coli were 91% and 83%, respectively...

    journal_title:Infection control and hospital epidemiology

    pub_type: 杂志文章

    doi:10.1086/529590

    authors: Lautenbach E,Santana E,Lee A,Tolomeo P,Black N,Babson A,Perencevich EN,Harris AD,Smith CA,Maslow J

    更新日期:2008-04-01 00:00:00