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Binding of the natural substrates and products to KDO8P synthase: 31P and 13C solution NMR characterization.

Abstract：

:Proton decoupled 31P and 13C solution NMR experiments were applied to mixtures of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthase, with each of its natural substrates, phosphoenolpyruvate and arabinose-5-phosphate (ASP), and product KDO8P to identify the formation of the enzyme-substrate and enzyme-product complexes. Effects arising from ligand interactions with the enzyme are reported via chemical shifts and line broadening with respect to those of the free ligands in solution, depending on the strength and dynamics of binding under thermodynamic equilibrium conditions. The characterization was done both at low and high field spectrometers, 200 and 500 MHz (1H frequencies), and in cases of 31P NMR measurements, it was demonstrated that only the low field spectrometer is capable of providing direct experimental evidence on the enzyme-ligand interactions. Since both the substrate A5P and the product KDO8P exhibit multiple anomeric forms in solution, evidence for the preference of recognition and binding of particular forms is sought.

journal_name

Bioorg Chem

journal_title

Bioorganic chemistry

authors

Kaustov L,Baasov T,Schmidt A

doi

10.1016/s0045-2068(03)00064-6

subject

Has Abstract

pub_date

2003-08-01 00:00:00

pages

306-21

issue

4

eissn

0045-2068

issn

1090-2120

pii

S0045206803000646

journal_volume

31

pub_type

杂志文章

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