Comparison of in vivo lymphocyte proliferation between allogeneic and xenogeneic heart transplantation in mice.

Abstract:

:There are controversial in vitro data comparing the strength of the cellular immune response between allogeneic and xenogeneic stimulator/responder combinations. The present study therefore compares in vivo lymphocyte proliferation using heart transplantation (HTx) models in mice. Heterotopic HTx into BALB/c mice was performed using donor organs from mice (BALB/c and C57BL/6) or Lewis rats. Intraperitoneally given bromodeoxyuridine (BrdU) was incorporated into the DNA and was subsequently analyzed by flow cytometry. On postoperative days 3 and 5, proliferation of splenocytes, CD4(+) T-lymphocytes, and CD19(+) B-lymphocytes was significantly higher after xenogeneic than after allogeneic and isogeneic HTx. No significant difference was observed when proliferation of CD8(+) lymphocytes was determined. The increased in vivo proliferation after xenotransplantation may reflect an earlier and probably stronger cellular immune response compared to allogeneic transplantation. The higher CD4(+) lymphocyte proliferation underscores the importance of this cell population in xenograft rejection.

journal_name

Microsurgery

journal_title

Microsurgery

authors

Kellersmann R,Kellersmann A,Thiede A,Ulrichs K

doi

10.1002/micr.10183

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

498-502

issue

5

eissn

0738-1085

issn

1098-2752

journal_volume

23

pub_type

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