Artificial biological ligament: its making, testing, and experimental study on animals.

Abstract:

:By applying a novel biochemical technique using porcine tendon as the raw material with its antigen minimized, we developed an artificial biological ligament (ABL). We examined and tested its structure, mechanical properties, and biocompatibility and explored the feasibility of reconstructing the anterior cruciate ligament (ACL) with ABL. By means of treating porcine tendon with epoxy crosslinking fixation, diversified antigen minimization process, mechanic enhancement modification, and surface activating process, we fabricated the ABL samples. We then analyzed its in vivo and in vitro performances, respectively, with animal (goat) implantation, histological examination, scanning electron microscope, cells culture, and mechanical tests before and after animal implants. The appearance of ABL was similar to that of normal human ligament. Histological examination showed that the ABL was composed of collagen fibers with no cells. Electron microscope examination revealed that the ABL was composed of hair-appearing and fiber-like objects running uniformly in a certain direction and closely parallel-arranged. Three weeks after xenogenic marrow matrix cells were cultured on the surface of the ABL, it was noted that cells adhered and the matrix secreted by the cells precipitated around the cells. There were no cells found inside the ABL. The average diameter of ABL was 5 mm and the mechanical test at a speed of 100 mm/min showed that its average tensile limit was 927.19 N; the tension-resistant strength was 47.22 N/mm. Those measurements were close to the corresponding parameters of the normal goat ACL. Twelve weeks after ABL replacement of the goat ACL, synovial membrane covering with the ingrowths of small blood vessels was seen on the surface of the implant. Fifty weeks after the replacement, the ABL material was partially replaced by regenerated host ligament-like tissue. Around the ABL material fibers in the bone tunnel close to the joint surface the direct connection mode, ligament-fibro cartilage-calcified cartilage-bone, was seen. As we used the unique biochemical technique and minimized the xenogenic protein immunogenicity of the porcine tendon, ABL showed acceptable biomechanical properties and superior biocompatibility. As a substitute for ligament in the reconstruction of the ACL, ABL has a promising prospect in clinical applications.

journal_name

Microsurgery

journal_title

Microsurgery

authors

Wang K,Zhu L,Cai D,Zeng C,Lu H,Xu G,Guo X,Lin S,Cheng S

doi

10.1002/micr.20442

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

44-53

issue

1

eissn

0738-1085

issn

1098-2752

journal_volume

28

pub_type

杂志文章
  • On sampling and sampling errors in histomorphometry of peripheral nerve fibers.

    abstract::Histomorphometrical assessment of regenerated peripheral nerves is a very common goal of many studies in experimental microsurgery. In this paper, the main critical issues in nerve fiber sampling for quantitative morphological assessment are addressed. The equal opportunity rule, i.e., the basic paradigm of random sam...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.10199

    authors: Geuna S,Gigo-Benato D,Rodrigues Ade C

    更新日期:2004-01-01 00:00:00

  • Contralateral C7 nerve root transfer in treatment of cerebral palsy in a child: case report.

    abstract::A 4-year-old girl who sustained the hemiplegic cerebral palsy and subsequent spasticity in the left upper extremity underwent the C7 nerve root rhizotomy and the contralateral C7 nerve root transfer to the ipsilateral middle trunk of brachial plexus through an interpositional sural nerve graft. In a 2-year follow-up, ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20877

    authors: Xu WD,Hua XY,Zheng MX,Xu JG,Gu YD

    更新日期:2011-07-01 00:00:00

  • Use of the venous flap for salvage of difficult ring avulsion injuries.

    abstract::Ring avulsion can lead to soft tissue compromise resulting in eventual digit failure or restriction of motion. The authors present use of the venous flow through flap for simultaneous soft tissue and digital vessel reconstruction in severe ring avulsion injury. A retrospective review of ring avulsion injuries treated ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20527

    authors: Brooks D,Buntic RF,Taylor C

    更新日期:2008-01-01 00:00:00

  • Microsurgical second dorsal metacarpal artery cutaneous and tenocutaneous flap for distal finger reconstruction: anatomic study and clinical application.

    abstract::In this paper, we report on the anatomical study of 34 cadaveric forearms with red latex injection and the clinical application of this study to 11 cases of microsurgical second dorsal metacarpal artery (SDMA) flaps. There were 8 cutaneous cases and 3 tenocutaneous cases using SDMA flaps for distal finger reconstructi...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20077

    authors: Yu GR,Yuan F,Chang SM,Zhang F

    更新日期:2005-01-01 00:00:00

  • Reverse-flow latissimus dorsi myocutaneous flap in a multi-step approach for complex back defect reconstruction: A case report.

    abstract::Reconstruction of complex back defects is challenging for reconstructive surgeons, as it should preserve function, provide adequate coverage, and minimize morbidity. We present a case of multiple-step reconstruction after resection of a large squamous cell carcinoma recurrence in a 68-year-old man, with local perforat...

    journal_title:Microsurgery

    pub_type:

    doi:10.1002/micr.30615

    authors: Menichini G,Lucattelli E,Innocenti A,Brogi M,Cipriani F,Innocenti M

    更新日期:2020-07-01 00:00:00

  • Natural history of digital replantation: a 12-year prospective study.

    abstract:UNLABELLED:The purpose of the study was to evaluate the fate of the digital arterial anastomoses, resting total digit blood flow, and cold-induced discomfort following digital replantation with two repaired arteries. The evaluation was done with a Doppler ultrasound of the anastomoses, temperature recording from the fi...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920160304

    authors: Povlsen B,Nylander G,Nylander E

    更新日期:1995-01-01 00:00:00

  • Vascularized fibular grafting for treatment of aseptic necrosis of the femoral head--preliminary results in four cases.

    abstract::Vascularized grafting with segments of fibula was performed in four men with aseptic necrosis of the femoral head. The patients were restricted to relatively young individuals in whom the necrosis seemed to be confined to two-thirds or less of the femoral head and in whom the outer shell of the femoral head appeared r...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920040108

    authors: Fujimaki A,Yamauchi Y

    更新日期:1983-01-01 00:00:00

  • Value of a dynamic MR scan in predicting vascular ingrowth from free vascularized scapular transplant used for treatment of avascular femoral head necrosis.

    abstract::We have treated three patients with avascular osteonecrosis using vascularized scapular bone graft. To predict blood perfusion in both the diseased femoral head and the transferred bone, all the hips were followed up using dynamic magnetic resonance (MR) scans, performed 1 and 7 months after surgery. In the present ca...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920161004

    authors: Fujisawa K,Hirata H,Inada H,Morita A,Takeda K,Hibasami H

    更新日期:1995-01-01 00:00:00

  • Stereomicroscopy as a diagnostic aid in evaluating the viability of island flaps: an experimental study.

    abstract::This study entailed direct stereomicroscopic observations of rat abdominal island flaps and the sequence of vascular events that occurred during and after occlusion by clamping of the individual femoral vessels for varying time intervals of up to 16 hours. The vascular changes and the status of the circulation in the ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920040205

    authors: Ballantyne DL,Razaboni RM,Converse JM

    更新日期:1983-01-01 00:00:00

  • The lymphatic superficial circumflex iliac vessels deep branch perforator flap: A new preventive approach to lower limb lymphedema after groin dissection-preliminary evidence.

    abstract:OBJECTIVE:Groin dissection is the procedure with the highest risk of lower limb lymphedema.As lymph stasis causes irreversible alterations to the limb over time,therapies should be administered in early stages,or better yet, lymphatic drainage impairment should be prevented.We developed a new preventive approach to lym...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.30142

    authors: Gentileschi S,Servillo M,Garganese G,Fragomeni S,De Bonis F,Cina A,Scambia G,Salgarello M

    更新日期:2017-09-01 00:00:00

  • A novel salvage approach for pedicle axial flap--free-ization of pedicle internal mammary artery perforator flap.

    abstract::A pedicle flap with distal segment compromise is classically managed by allowing tissue demarcation, debridement of non-viable tissue, and local tissue manipulation to achieve wound closure. When aggressive debridement leaves insufficient tissue for defect coverage, the original flap is often discarded. We present a c...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.21962

    authors: Chen WF,Tsao CK,Cheng MH

    更新日期:2012-05-01 00:00:00

  • Early survival of a compromised fasciocutaneous flap without pedicle revision: monitoring with photoplethysmography.

    abstract::While free flaps are reliant on their vascular pedicle for survival intraoperatively and for a variable period of time postoperatively, there have been reports of late pedicle compromise after which complete flap survival has ensued. Successful neovascularization and revascularization at the edges of a flap in such ca...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20781

    authors: Chubb D,Rozen WM,Ashton MW

    更新日期:2010-09-01 00:00:00

  • Addition of nitric oxide donor S-nitroso-N-acetylcysteine to selective iNOS inhibitor 1400W further improves contractile function in reperfused skeletal muscle.

    abstract::This study examines the effects of combination therapy with the nitric oxide (NO) donor S-nitroso-N-acetylcysteine (SNAC) and the iNOS inhibitor N-(3-(aminomethyl)benzyl) acetamidine (1400W) on contractile function in reperfused rat skeletal muscle. The right extensor digitorum longus (EDL) muscles of 104 rats were su...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20122

    authors: Barker JU,Qi WN,Cai Y,Urbaniak JR,Chen LE

    更新日期:2005-01-01 00:00:00

  • A new microvascular clamp for the production of experimental ischemia.

    abstract::A new microvascular clamp was developed from inexpensive and readily available materials. This clamp, when properly applied, can provide many hours of vessel occlusion with minimal damage to the vessel intima. This clamp has important applications in producing prolonged experimental ischemia without significant vessel...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920050407

    authors: Stark RH,Sanger JR,Matloub HS

    更新日期:1984-01-01 00:00:00

  • Multifactorial effects on the patency rates of forearm arterial repairs.

    abstract::It is clear that the late clinical symptomatology and the patency of forearm arterial repairs have been contradictory. This study, during which the relationship between the symptomatology and patency has been studied, explores the influence of the local hemodynamic changes and the effect of microsurgical technique on ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1006

    authors: Bacakoğlu A,Ozkan MH,Coşkunol E,Ozdemir O,Ekin A

    更新日期:2001-01-01 00:00:00

  • Entrapment of the median nerve after posterior dislocation of the elbow.

    abstract::The authors present two cases of complete median nerve paralysis due to entrapment after posterior dislocation of the elbow. Previous literature on the subject matter is reviewed, and management and therapeutic indication of these lesions are discussed. ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920040403

    authors: Ayala H,De Pablos J,Gonzalez J,Martinez A

    更新日期:1983-01-01 00:00:00

  • Ethical considerations in composite tissue allotransplantation.

    abstract::Ethical issues posed by the hand transplant program conducted by hand and plastic surgeons at Jewish and University Hospitals, Louisville, Kentucky, are examined in this essay. Because a composite tissue allotransplantation (CTA) is an experimental procedure, it raises issues as to the protection of human subjects. Th...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/1098-2752(2000)20:8<458::aid-micr19>3.0.co

    authors: Simmons PD

    更新日期:2000-01-01 00:00:00

  • Reduction of reperfusion injury edema with corticotropin-releasing factor (CRF): a pilot study.

    abstract::Regulation of capillary permeability with ischemic reperfusion injury is a complex interaction between vascular endothelium and circulating blood factors. Corticotropin-releasing factor (CRF), a 41 -amino acid peptide CNS neurotransmitter known to modulate the pituitary-adrenal axis during stress response, has been sh...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-2752(1998)18:2<76::aid-micr3>3.

    authors: Whitney TM,Bentz ML,Johnson PC

    更新日期:1998-01-01 00:00:00

  • Evaluation of the effect of pharmacologic agents on crush-avulsion arterial injuries: a scanning electron microscopy study.

    abstract::This study was designed to investigate the short-term formation and composition of thrombus occurring at sites of arterial intimal injury in rabbits. Anti-coagulants, platelet anti-aggregation agents, and fibrinolytic agents were evaluated for their influence upon the developing thrombus, utilizing scanning electron m...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920120413

    authors: Savoie FH,Cooley BC,Gould JS

    更新日期:1991-01-01 00:00:00

  • Correlation of contractile function recovery with acetylcholine receptor changes in a rat muscle flap model.

    abstract::The aim of this study was to investigate the correlation between contractile function recovery and changes of acetylcholine receptors (AChR) in a transferred muscle flap following reinnervation. Orthotopic transfer of the gracilis muscle flap with repair of its nerve was performed bilaterally in 48 rats. The rats were...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20727

    authors: Zhang Y,Liu A,Zhang W,Jiang H,Cai Z

    更新日期:2010-05-01 00:00:00

  • Relationship between lymphatic and venous pressure in normal and lymphedematous rabbit ears.

    abstract::Studies of lymphatic and venous pressures of normal and lymphedematous rabbit ears reveal the end pressure of lymphatics to be higher than the venous pressure. Massage of the related rabbit ear increases the lymphatic pressures and can markedly raise the side and end pressures within the lymphatic wall. The venous pre...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920050108

    authors: Huang GK,Hsin YP

    更新日期:1984-01-01 00:00:00

  • Reconstruction of peripheral nerves using acellular nerve grafts with implanted cultured Schwann cells.

    abstract::The bridging of nerve gaps is still one of the major problems in peripheral nerve surgery. The present experiment describes our attempt to engineer different biologic nerve grafts in a rat sciatic nerve model: cultured isogenic Schwann cells were implanted into 2-cm autologous acellular nerve grafts or autologous pred...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.10056

    authors: Frerichs O,Fansa H,Schicht C,Wolf G,Schneider W,Keilhoff G

    更新日期:2002-01-01 00:00:00

  • Morphometric changes in free neurovascular latissimus dorsi flaps: an experimental study.

    abstract::This study was designed to investigate regeneration of reinnervated, free transplanted muscles. We used a rat model, consisting of eight rats per group, in which the latissimus dorsi muscle was transplanted orthotopically and then harvested and evaluated after 2 and 12 weeks. Age-matched control animals were used to o...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920161205

    authors: Becker MH,Lassner F,Dagtekin FZ,Walter GF,Berger A

    更新日期:1995-01-01 00:00:00

  • The radial forearm free flap as a "vascular bridge" for secondary microsurgical head and neck reconstruction in a vessel-depleted neck.

    abstract:BACKGROUND:In a vessel-depleted neck, distant recipient sites may be the only option for secondary free flap reconstruction. While interposition vein grafts and arteriovenous loops can bridge the gap between the recipient and donor pedicle, they are not without risks. In these scenarios, we examinate the reliablity of ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.30259

    authors: Ciudad P,Agko M,Date S,Chang WL,Manrique OJ,Huang TCT,Lo Torto F,Trignano E,Chen HC

    更新日期:2018-09-01 00:00:00

  • Reversal of toe clawing in the patient with neuropathy by neurolysis of the distal tibial nerve.

    abstract::The distinction between hammer toes and clawed toes is poorly emphasized. The neural origin of clawing in the foot is related to the lateral plantar nerve, and suggests that surgical treatment directed at decompression or neurolysis of the lateral plantar nerve may either reverse or prevent progression of intrinsic mo...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20513

    authors: Dellon AL,Steck JK

    更新日期:2008-01-01 00:00:00

  • Double free-flap reconstruction of massive defects involving the lip, chin, and mandible.

    abstract::Two patients with massive, composite defects of the total lower lip, chin, and anterior mandible underwent double free-flap reconstruction. A fibular osteoseptocutaneous flap was used to reconstruct the mandible and floor of the mouth and a radial forearm fasciocutaneous composite flap, including the palmaris longus t...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-2752(1998)18:6<372::aid-micr6>3

    authors: Kuzon WM Jr,Jejurikar S,Wilkins EG,Swartz WM

    更新日期:1998-01-01 00:00:00

  • Objective preoperative estimation of the ability of primary closure on anterolateral thigh free flap donor site: circumference and area ratios of deep tissue to thigh.

    abstract:PURPOSE:The purpose of this study was to consider the relationship between the ratio of deep tissue including muscle to thigh at donor sites and the possibility of performing primary closure of donor site. METHODS:The subjects were 74 patients who had harvesting of anterolateral thigh (ALT) free flap from June 2005 to...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.22080

    authors: Song K,Nam S,Choi S,Kim J,Lee J,Bae Y

    更新日期:2013-05-01 00:00:00

  • New methods for objective angiogenesis evaluation of rat nerves using microcomputed tomography scanning and conventional photography.

    abstract:INTRODUCTION:Nerve regeneration involves multiple processes, which enhance blood supply that can be promoted by growth factors. Currently, tools are lacking to visualize the vascularization patterns in transplanted nerves in vivo. The purpose of this study was to describe three-dimensional visualization of the vascular...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.30537

    authors: Saffari TM,Mathot F,Bishop AT,Shin AY

    更新日期:2020-03-01 00:00:00

  • Omental free-tissue transfer: indications and results from personal experience.

    abstract::Between 1987-2002, omental free-tissue transfer was used in 11 patients, aged 6-65 years (mean age, 37.6). The omentum was used for the treatment of brachial plexus injury pain (3 cases), Romberg's disease (1 case), defects of the extremities occurring with chronic obstructive arterial disease (2 cases), posttraumatic...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.10130

    authors: Ciuce C,Seddiq F,Fodor M,Constantinescu D,Todoran M,Andercou A,Demco D

    更新日期:2003-01-01 00:00:00

  • A new technique for microvascular sleeve anastomosis.

    abstract::This study describes our experience using an improved technique for microsurgical sleeve anastomosis of arteries. Modifications of previously described techniques include utilization of a sidecut and placement of the two stitches within separate vertical planes. In 40 arteries, we attained a patency rate of 97.5%. His...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920120502

    authors: Zhang L,Tuchler RE,Shaw WW,Siebert JW

    更新日期:1991-01-01 00:00:00