The thermal history of human fossils and the likelihood of successful DNA amplification.

Abstract:

:Recent success in the amplification of ancient DNA (aDNA) from fossil humans has led to calls for further tests to be carried out on similar material. However, there has been little systematic research on the survival of DNA in the fossil record, even though the environment of the fossil is known to be of paramount importance for the survival of biomolecules over archaeological and geological timescales. A better understanding of aDNA survival would enable research to focus on material with greater chances of successful amplification, thus preventing the unnecessary loss of material and valuable researcher time. We argue that the thermal history of a fossil is a key parameter for the survival of biomolecules. The thermal history of a number of northwest European Neanderthal cave sites is reconstructed here and they are ranked in terms of the relative likelihood of aDNA survival at the sites, under the assumption that DNA depurination is the principal mechanism of degradation. The claims of aDNA amplification from material found at Lake Mungo, Australia, are also considered in the light of the thermal history of this site.

journal_name

J Hum Evol

authors

Smith CI,Chamberlain AT,Riley MS,Stringer C,Collins MJ

doi

10.1016/s0047-2484(03)00106-4

subject

Has Abstract

pub_date

2003-09-01 00:00:00

pages

203-17

issue

3

eissn

0047-2484

issn

1095-8606

pii

S0047248403001064

journal_volume

45

pub_type

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