Abstract：
OBJECTIVE:The objective was to test the reliability of new deoxyribonucleic acid primers that have previously been used very efficiently by this laboratory with amniolysate samples to amplify a 248 bp Y-specific, repeated sequence from maternal blood during pregnancy. STUDY DESIGN:Blood samples were obtained from 50 women during weeks 11 and 16 of pregnancy and were analyzed for the presence of the Y chromosome-specific sequences. RESULTS:Y-specific fragments were identified in 19 of 24 (79.2%) women after one complete amplification. A second amplification of these samples negative for Y-specific fragments revealed three additional samples positive for the Y chromosome-specific fragment. Only two male fetuses remained unidentified. Overall, 91.7% male fetuses and 96% of all fetuses (48/50) in these women were correctly identified. CONCLUSIONS:The primers described in this study provide an additional or alternative tool for the determination, by means of the polymerase chain reaction, of Y chromosome-bearing cells in maternal circulation.
journal_name
Am J Obstet Gynecoljournal_title
American journal of obstetrics and gynecologyauthors
Adkison LR,Andrews RH,Vowell NL,Koontz WLdoi
10.1016/s0002-9378(94)70312-4subject
Has Abstractpub_date
1994-03-01 00:00:00pages
952-5issue
3eissn
0002-9378issn
1097-6868pii
S0002937894005272journal_volume
170pub_type
杂志文章abstract：:Tubal biopsy specimens were obtained at the time of tubal anastomosis in 26 previously sterilized women. Scanning electron microscopy showed that half the patients had abnormalities of tubal mucosa, including loss of mucosal folds, deciliation, and polyposis. These pathologic conditions, the morphologic features of wh...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(80)90015-0
更新日期：1980-09-01 00:00:00
abstract：OBJECTIVE:Our aim was to compare plasma drug levels in patients receiving ritodrine intravenously with those in patients receiving ritodrine orally at recommended dosages. STUDY DESIGN:Plasma samples from 20 pregnant patients treated with intravenous ritodrine (50 to 300 micrograms/min), 9 patients treated with oral r...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(93)90054-m
更新日期：1993-10-01 00:00:00
abstract：OBJECTIVE:Ovarian vein thrombosis is associated with pregnancy and pelvic surgery. Postpartum ovarian vein thrombosis is associated with infection and a high morbidity rate and is treated with anticoagulant and intravenous antibiotic therapy. The natural history of such thrombotic events after debulking surgery for ova...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2015.02.028
更新日期：2015-08-01 00:00:00
abstract：:In this article we report the results of chorionic villus sampling by a biopsy forceps inserted via the cervix under ultrasonic guidance in 300 pregnancies at risk for thalassemia major. A sufficient amount of chorionic villi for deoxyribonucleic acid analysis by oligonucleotide hybridization was obtained in all cases...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(87)90352-8
更新日期：1987-04-01 00:00:00
abstract：:A new colposcopic sign (sharpness of peripheral margins) was graded into three objective categories representing subclinical papillomaviral infection, lower grade dysplasia, and grade 3 cervical intraepithelial neoplasia. Colposcopic features were prospectively recorded in 72 women and then correlated with histologic ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(85)80244-1
更新日期：1985-11-15 00:00:00
abstract：OBJECTIVE:The risk of perinatal death associated with labor after previous cesarean section appears higher than with a repeated cesarean section. On the other hand, repeated cesarean sections are associated with increased maternal morbidity and mortality from placental pathologic conditions (previa or accreta) on subse...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1067/s0002-9378(03)00833-0
更新日期：2003-09-01 00:00:00
abstract：OBJECTIVE:The objective of the study was to examine the association in humans between maternal psychosocial stress exposure during pregnancy and measures of glucose-insulin metabolism in the adult offspring. STUDY DESIGN:Healthy young adults whose mothers experienced major stressful life events during their pregnancy ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2008.03.006
更新日期：2008-11-01 00:00:00
abstract：:This paper calls attention to rapidly increased use of vertical uterine incision and future consequences: impaired uterus, uterine rupture, and iatrogenic premature delivery. In addition, by making a vertical uterine incision we are not gaining much in the size of uterine entrance since the size of the vertical uterin...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(85)90541-1
更新日期：1985-08-15 00:00:00
abstract：OBJECTIVE:The objective of the study was to compare 3 different approaches for increasing clients' understanding of contraceptive effectiveness. STUDY DESIGN:We randomized 900 reproductive-age women in India and Jamaica to 1 of 3 charts presenting pregnancy risk. RESULTS:The most important reason for choosing a contr...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.ajog.2005.12.053
更新日期：2006-07-01 00:00:00
abstract：OBJECTIVE:Our purpose was to study platelet size and surface glycoprotein expression in normal fetal and maternal blood throughout pregnancy. STUDY DESIGN:A cross-sectional study was performed at the Harris Birthright Research Centre for Fetal Medicine, King's College Hospital Medical School, London. Fetal and materna...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(94)90099-x
更新日期：1994-09-01 00:00:00
abstract：:Previous studies in our laboratory have suggested that measurement of methylumbelliferylguanidinobenzoate (MUGB) reactive proteases in midtrimester amniotic fluid is potentially of value for the intrauterine detection of cystic fibrosis. Thirty-nine pregnancies of obligate heterozygotes of cystic fibrosis and 12 cases...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(16)32679-5
更新日期：1981-12-15 00:00:00
abstract：:Fetal movements were directly visualized with a real-time ultrasound unit, and they were recorded after the random intravenous administration of either glucose or saline solution to the mother. A significant increase in fetal activity was seen 1 hour after the administration of glucose. ...
journal_title：American journal of obstetrics and gynecology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0002-9378(80)91128-x
更新日期：1980-06-15 00:00:00
abstract：:The purpose of this study was to evaluate the transfer constant of conversion of plasma androstenedione to estrone ([p]AE1BU) as a function of total body weight and excess body weight in ovulatory and anovulatory young adult women. Twenty-four ovulatory and 31 anovulatory women were studied; their weights ranged from ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(78)90288-0
更新日期：1978-02-15 00:00:00
abstract：:Pure long-term cytotrophoblast cultures were established from human first-trimester placentas by growing chorionic villus explants without enzymatic digestion. Cytoplasmic human chorionic gonadotropin was detectable in all (100%) cells in culture when labeled with a polyclonal anti-human chorionic gonadotropin antibod...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(89)90314-1
更新日期：1989-04-01 00:00:00
abstract：OBJECTIVE:The purpose of our study was to examine the neuronal outcome after a standardized period of umbilical cord occlusion. STUDY DESIGN:The umbilical cord was clamped for 10 minutes in nine experimental and four control chronically instrumented fetal sheep. Three days later the animals were killed for histologic ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(11)91728-1
更新日期：1992-11-01 00:00:00
abstract：:The progesterone antagonist onapristone was used in guinea pigs during late pregnancy (43 +/- 2 days after coitus) and before term (day 61 after coitus) to investigate the role of progesterone on uterine reactivity to exogenous oxytocin, concentration of oxytocin receptors, and gap junctions in the myometrium. Onapris...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(91)90030-u
更新日期：1991-12-01 00:00:00
abstract：:The intrauterine pressure data obtained from oxytocin-treated patients in dysfunctional labor, managed by a specific oxytocin regimen and a computer-defined goal of uterine activity, are analyzed. Questions asked concern the efficacy of the computer diagnosis of hypocontractility, the quantity of uterine activity need...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(83)91189-4
更新日期：1983-03-01 00:00:00
abstract：:From January 1, 1970 to July 1, 1976, 128 women had hydatidiform moles evacuated at the Los Angeles County-University of Southern California Women's Hospitals. Of the 121 patients with follow-up, persistent trophoblastic disease (TD) was diagnosed in 32 (26.4 per cent). Remission was achieved in all treated patients. ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(77)90562-2
更新日期：1977-06-15 00:00:00
abstract：OBJECTIVE:The purpose of this study was to identify differences in antepartum uterine contraction frequency (UCF) in twin pregnancies with and without preterm labor (PTL). STUDY DESIGN:Twin gestations enrolled for outpatient surveillance with twice daily electronic uterine activity monitoring and telephonic nursing as...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2007.10.781
更新日期：2008-05-01 00:00:00
abstract：:A relationship between serum thrombomodulin and preeclampsia was investigated. In women with severely preeclamptic pregnancies, serum thrombomodulin levels were found to be significantly elevated as compared with those of matched control subjects (p < 0.005). Serum thrombomodulin levels correlated positively with seru...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(93)90151-8
更新日期：1993-07-01 00:00:00
abstract：:Analysis of uncultured chorionic villus material from a woman at risk of fetus with sulfite oxidase deficiency revealed a deficiency of sulfite oxidase. This was confirmed on termination of the pregnancy. ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(90)90691-y
更新日期：1990-10-01 00:00:00
abstract：:We studied the effect of diabetic nephropathy on the course of pregnancy, perinatal outcome, and infant development and determined the influence of pregnancy on maternal hypertension and renal function. Maternal proteinuria usually increased during pregnancy (greater than 3 gm/24 hours in 69%), and hypertension was pr...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(81)90698-0
更新日期：1981-12-01 00:00:00
abstract：OBJECTIVE:Our purpose was to determine the effect of in utero exposure to indomethacin on the need for surgical closure of a patent ductus arteriosus (PDA). STUDY DESIGN:Perinatal variables were compared between infants at <32 weeks who required surgical closure of PDA after failed medical management and those who did...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1067/mob.2002.127464
更新日期：2002-10-01 00:00:00
abstract：:A 31-year-old woman with a positive pregnancy test and a transvaginal ultrasound scan result that was suggestive of a right tubal ectopic pregnancy underwent a laparoscopy, which showed bilateral hematosalpinx. In the presence of active bleeding and deteriorating hemodynamic status of the patient, a minilaparotomy was...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1067/s0002-9378(03)00206-0
更新日期：2003-09-01 00:00:00
abstract：:Pelvic blood flow was serially determined in 19 pregnant ewes before and after the induction of one-kidney renal hypertension. The six twin gestations and 13 singleton gestations behaved similarly for all parameters, except renal blood flow. After the renal preparatory surgical procedure, there was a 19% increase in m...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(16)32681-3
更新日期：1981-12-15 00:00:00
abstract：OBJECTIVE:The amount of bone mass and the tendency to fall are main risk factors for hip fractures and both deteriorate with advancing age. The dynamics between estrogen exposure and fracture protection seem too rapid to be explained by an effect on bone mass only. Postural balance function may be another potential mec...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(97)70207-2
更新日期：1997-08-01 00:00:00
abstract：OBJECTIVE:Review alternative approaches to oral metronidazole for the treatment of bacterial vaginosis. STUDY DESIGN:Assessment of clinical trials on the treatment of bacterial vaginosis with regimens other than 5 to 7 days of metronidazole. RESULTS:Single-dose metronidazole, 2 gm orally, is as effective as 5- to 7-d...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,评审
doi：10.1016/0002-9378(93)90346-k
更新日期：1993-08-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to examine the association between gestational age (GA) at the time of treatment initiation for gestational diabetes mellitus (GDM) and maternal and perinatal outcomes. STUDY DESIGN:We conducted a secondary analysis of a multicenter randomized treatment trial of mild GDM in whic...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.ajog.2015.06.022
更新日期：2015-10-01 00:00:00
abstract：:Maternal and associated neonatal mortality rates in sub-Saharan Africa remain unacceptably high. In Mulago Hospital (Kampala, Uganda), 2 major causes of maternal death are preeclampsia and obstructed labor and their complications, conditions occurring at the extremes of the birthweight spectrum, a situation encapsulat...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,评审
doi：10.1016/j.ajog.2013.10.879
更新日期：2014-06-01 00:00:00
abstract：OBJECTIVE:The primary objective of this study was to identify specific educational goals for obstetrics and gynecology residents in the ambulatory setting. STUDY DESIGN:A cross-sectional study of current practice patterns in primary care, benign gynecology, and office procedures was performed with mailings to local pr...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2004.07.070
更新日期：2004-11-01 00:00:00
