Abstract：
:An analytical method for nipecotic acid quantification in rat blood was developed utilizing a stable isotope internal standard and capillary gas chromatography-mass spectroscopy. The method involves a solid phase extraction step followed by a two-step derivatization. The analytes are separated by capillary gas chromatography and detected by selected ion monitoring of their base peaks at 180 and 185 m/z, respectively. The assay has a limit of detection (LOD) of 10 ng/ml and a limit of quantification of 26 ng/ml in 200 microl of rat whole blood. The linear range of the assay covers from 26 to 6500 ng/ml (r2=0.9996, n=9). The coefficient of variation was less than 10% at concentrations of 50, 1000 and 5000 ng/ml. The assay was used to characterize the pharmacokinetics of R-(-)-nipecotic acid in a rat. R-(-)-nipecotic acid clearance was 4.2 ml/min, its half-life was 1.5h and its volume of distribution at steady state was 325 ml.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Wang H,Hussain AA,Pyrek JS,Goodman J,Wedlund PJdoi
10.1016/j.jpba.2003.11.019subject
Has Abstractpub_date
2004-03-10 00:00:00pages
1063-70issue
5eissn
0731-7085issn
1873-264Xpii
S0731708503006666journal_volume
34pub_type
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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