Abstract：
:Specific enzymatic and chemical degradation of neutral lipid and phospholipid fractions from rat liver revealed the presence of novel types of lipid metabolites bearing a short-chain diol backbone. Diol-derived lecithin and cephalin analogs were readily cleaved by phospholipase C (EC 3.1.4.3) from Bacillus cereus, although the cephalin analogs required "carrier" lecithin to sustain hydrolysis. The products of phosphilipase hydrolyses as well as the neutral lipid fractions were subjected to alkaline and acidic methanolysis, and constituent short-chain diols were analyzed as long-chain cyclic acetals. Gas chromatographymass spectrometry confirmed that 1,2-ethanediol, 1,2-propanediol, 1,3-propanediol, and 1,3 butanediol can form the polyol backbone of neutral lipids and phospholipids. [1,1,2,2-2H]Ethanediol monohexadecanoate, dihexadecanoate, hexadecanoylphosphorylcholine, hexadecanoylphosphorylethanolamine were synthesized chemically and served as internal standards to assure accurate quantitation of the low levels of diol lipids (350 mug/g ot total lipid) present in rat liver.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Baumann WJ,Schupp E,Lin JTdoi
10.1021/bi00675a031subject
Has Abstractpub_date
1975-02-25 00:00:00pages
841-7issue
4eissn
0006-2960issn
1520-4995journal_volume
14pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The genomic and antigenomic RNAs of hepatitis delta virus are capable of self-cleavage and show no significant sequence similarities to other known self-cleaving RNAs. We have derived an antigenomic delta RNA which cleaves to completion in 15 s in 9 mM magnesium at 37 degrees C and is capable of efficient self-cleavag...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00155a015
更新日期：1992-10-13 00:00:00
abstract：:A total of fifty single site surface phenylalanine substitution mutants have been made in the model protein staphylococcal nuclease. The fifty residues that were replaced with phenylalanine were chosen to give a broad sampling of solvent accessibility, secondary structure, and backbone conformations. The change in the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9725069
更新日期：1998-05-12 00:00:00
abstract：:A study of the dynamics and mechanism of the various thermotropic phase transitions undergone by the hydrated monoacylglycerides monoolein and monoelaidin, in the temperature range of 20-120 degrees C and from 0 to 5 M NaCl, has been undertaken. Measurements were made by using time-resolved X-ray diffraction at the Co...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00394a008
更新日期：1987-10-06 00:00:00
abstract：:The erythropoietin (EPO) molecule contains four carbohydrate chains. Three contain N-linkages to asparagines at positions 24, 38, and 83, and one contains an O-linkage to a serine at position 126. We constructed human EPO variants that eliminated the three N-glycosylation sites by replacing the asparagines with glutam...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00156a003
更新日期：1992-10-20 00:00:00
abstract：:In bacteria, the transcription of virulence genes is usually controlled by a cell density-dependent process known as "quorum sensing" (QS). QS relies on small diffusible signaling molecules that cross the bacterial cell wall and activate target transcription factors after a threshold concentration has been reached. Be...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900123j
更新日期：2009-11-03 00:00:00
abstract：:Transfection of cos-1 cells with either prostaglandin endoperoxide H synthase-1 (PGHS-1) or -2 (PGHS-2) results in a mixed population of cells containing a diverse range of expressed enzyme. The use of fluorescent substrates and antibodies, in conjunction with fluorescence microscopy, provides the means to quantitate ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00021a034
更新日期：1995-05-30 00:00:00
abstract：:Metabolism of [2-13C]-, [3-13C]-, and [1,2,3-13C]propionate in perfused rat livers and [2-13C]-acetate in perfused rat hearts has been examined in tissue extracts by 13C NMR. Label from [2-13C]-propionate was preferentially incorporated into the C2 carbon of lactate, alanine, and aspartate in liver tissue while label ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00186a029
更新日期：1994-05-24 00:00:00
abstract：:Toxic shock syndrome toxin-1 (TSST-1), the potent staphylococcal exoprotein linked to most cases of the toxic shock syndrome, is a V beta-restricted T-cell mitogen (a so-called "superantigen"). TSST-ovine (TSST-O) is a natural variant of TSST-1, and is produced by certain ovine mastitis-associated strains of Staphyloc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00209a016
更新日期：1994-11-01 00:00:00
abstract：:There is no complete understanding of how serine protease inhibitors of the serpin family inhibit their target enzymes. Structural and biochemical studies have suggested that serpins utilize a mechanism that is distinct from the standard mechanism of inhibition proposed for most small protein protease inhibitors. Prot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960233w
更新日期：1996-06-11 00:00:00
abstract：:This paper reports the first identification of a fully functional hydrolyzing UDP-N-acetylglucosamine 2-epimerase from a bacterial source. The epimerase (known as SiaA or NeuC) from Neisseria meningitidis MC58 group B is shown to catalyze the conversion of UDP-GlcNAc into ManNAc and UDP in the first step of sialic aci...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048606d
更新日期：2004-11-09 00:00:00
abstract：:In this work we modeled the circular dichroism (CD) spectrum of LHCII, the main light harvesting antenna of photosystem II of higher plants. Excitonic calculations are performed for a monomeric subunit, taken from the crystal structure of trimeric LHCII from spinach [Liu, Z. F., Yan, H. C., Wang, K. B., Kuang, T. Y., ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi062031y
更新日期：2007-04-24 00:00:00
abstract：:The agouti-related protein gene (Agrp) plays an important role in body weight regulation. The mature human protein is a single polypeptide chain of 112 amino acid residues, consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain. The disulfide structure of recombinant human AGRP was dete...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981082v
更新日期：1998-09-01 00:00:00
abstract：:The aggregation of the protein tau into amyloid fibrils is known to be involved in the causation of the neurodegenerative tauopathies and the progression of cognitive decline in Alzheimer's disease. This review surveys the mechanism of tau aggregation with special emphasis on the information obtained from biochemical ...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi400209z
更新日期：2013-06-18 00:00:00
abstract：:Amyloid beta-protein (Abeta) self-assembly is linked strongly to Alzheimer's disease. We found that PP-Leu, a tridecapeptide analogue of broad-spectrum antiviral peptides termed theta-defensins, potently inhibits Abeta oligomer and fibril formation. This effect appeared to be mediated through sequestration of the amyl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901325g
更新日期：2009-12-08 00:00:00
abstract：:The energy derived from optimized van der Waals interactions in closely packed, folded proteins has been proposed to be of similar energetic magnitude to hydrophobicity in stabilizing the native state. If packing is this energetically important, it should influence the evolution of protein core sequences. To test this...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011776v
更新日期：2001-12-18 00:00:00
abstract：:The mechanism by which some hydrophobic molecules such as steroids and free fatty acids (FFA) act as noncompetitive inhibitors of the nicotinic acetylcholine receptor (AChR) is still not known. In the present work, we employ Förster resonance energy transfer (FRET) between the intrinsic fluorescence of membrane-bound ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061388z
更新日期：2007-03-20 00:00:00
abstract：:Heparin accelerates inhibition of factor XIa (fXIa) by the serpins antithrombin (AT) and C1-inhibitor (C1-INH) by more than 2 orders of magnitude. The mechanism of the heparin-mediated acceleration of fXIa inhibition by these serpins is incompletely understood, as heparin appears to interact with both the catalytic an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi802298r
更新日期：2009-02-24 00:00:00
abstract：:Regions of the negative strands of the satellite RNAs of chicory yellow mottle virus (sCYMV1) and arabis mosaic virus (sArMV) have similarity in sequence and predicted secondary structure compared to the tobacco ringspot virus satellite RNA (sTRSV) hairpin ribozyme, suggesting that they may also be catalytic RNAs of a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00048a024
更新日期：1995-12-05 00:00:00
abstract：:Glycogen phosphorylase is a muscle enzyme which metabolizes glycogen, producing glucose-1-phosphate, which can be used for the production of ATP. Phosphorylase activity is regulated by phosphorylation/dephosphorylation, and by the allosteric binding of numerous effectors. In this work, we have studied 10 site-directed...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0020372
更新日期：2001-05-01 00:00:00
abstract：:One cause of multidrug resistance is the overexpression of P-glycoprotein, a 170 kDa plasma membrane ABC transporter, which functions as an ATP-driven efflux pump with broad specificity for hydrophobic drugs, peptides, and natural products. The protein appears to interact with its substrates within the membrane enviro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0485326
更新日期：2005-01-18 00:00:00
abstract：:Chicken liver phosphoenolpyruvate carboxykinase (PEPCK) was rapidly inactivated by micromolar concentrations of ferrous sulfate in the presence of ascorbate at pH 7.4. Omitting ascorbate or replacing the Fe2+ with Mn2+ or Mg2+ gives no inactivation. Mn2+, Mg2+, or Co2+ at 100-fold molar excess over Fe2+ offered comple...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970574p
更新日期：1997-12-09 00:00:00
abstract：:We have determined the structures of three novel oligosaccharides isolated from human milk using the monoclonal antibody MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography. From the results of 500-MHz...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00448a031
更新日期：1989-10-31 00:00:00
abstract：:Intrinsic disorder is at the center of biochemical regulation and is particularly overrepresented among the often multifunctional viral proteins. Replication and transcription of the respiratory syncytial virus (RSV) relies on a RNA polymerase complex with a phosphoprotein cofactor P as the structural scaffold, which ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b01332
更新日期：2016-03-15 00:00:00
abstract：:It is known that, while melittin at micromolar concentrations is unfolded under conditions of low ionic strength at neutral pH, it adopts a tetrameric alpha-helical structure under conditions of high ionic strength, at alkaline pH, or at high peptide concentrations. To understand the mechanism of the conformational tr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00118a014
更新日期：1992-01-28 00:00:00
abstract：:The prothrombinase complex is comprised of an enzyme, factor Xa, and a cofactor, factor Va, that each bind peripherally to membranes containing phosphatidylserine (PS) and activate the substrate, prothrombin. The mechanism by which the membrane contributes to enhanced catalytic efficacy of prothrombinase is not precis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047655n
更新日期：2005-03-15 00:00:00
abstract：:Soluble guanylate cyclase (sGC) is a heterodimeric hemoprotein composed of alpha1 and beta1 subunits. sGC is activated by nitric oxide (NO) and therefore plays a central role in NO signal transduction. Activation of sGC by NO is believed to be mediated by the interaction between NO and the heme of sGC. Spectroscopic a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971825x
更新日期：1997-12-16 00:00:00
abstract：:PACT is a stress-modulated activator of interferon (IFN)-induced double-stranded (ds) RNA-activated protein kinase (PKR) and is an important regulator of PKR-dependent signaling pathways. Stress-induced phosphorylation of PACT is essential for PACT's association with PKR leading to PKR activation. PKR activation by PA...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200104h
更新日期：2011-05-31 00:00:00
abstract：:Ner repressors of the transposable phages Mu and D108 play a central role in regulating the expression of the early (transposase) operon and in ensuring that phage growth proceeds along a lytic pathway. The latter function is analogous to that performed by the Cro protein of phage lambda. Unlike lambda Cro, however, t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00201a018
更新日期：1994-09-06 00:00:00
abstract：:The PB1 domain of NBR1 folds via a single pathway mechanism involving two sequential energy barriers separated by a high-energy intermediate. The structural ensemble representing each of the two transition states (TS1 and TS2) has been calculated using experimental Φ values and biased molecular dynamics simulations. B...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1016793
更新日期：2011-01-11 00:00:00
abstract：:Mycoplasmas are the smallest known microorganisms, with drastically reduced genome sizes. One of the essential biochemical pathways lost in mycoplasmas is methylation-mediated DNA repair (MMR), which is responsible for correction of base substitutions, insertions, and deletions in both bacteria and higher organisms. W...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2009097
更新日期：2011-10-11 00:00:00
