OK432 inhibits experimental hepatic metastasis of colon adenocarcinoma ACL-15 in F344 rats.

Abstract:

:The effect of OK432 on hepatic metastasis, induced by inoculating 1 x 10(6) ACL-15 cells from a rat colon adenocarcinoma cell line into the ileocolic vein of male F344 rats, was investigated in this study. Metastases were detected 14 days after inoculation in the control rats, however, pretreatment 3 days prior to the tumor cell inoculation with an anti-asialoGM1 antibody, which eliminates natural killer (NK) cell activity in vitro, increased the number of hepatic metastases, shortened the survival time, and decreased the NK activity of the nonparenchymal liver cells (NPC). In contrast, pretreatment with OK432 2 days prior to tumor inoculation significantly decreased the number of hepatic metastases, prolonged the survival time, and augmented the NK activity of the NPC, although treatment with OK432 3 or 7 days after inoculation did not decrease the number of hepatic metastases. Moreover, NPC from the OK432-pretreated rats had a marked antitumor effect against ACL-15 cells in the Winn's neutralization test. The results of this study indicate that pretreatment with OK432 before tumor cell inoculation inhibits hepatic metastasis in this experimental model, possibly by augmentating liver-associated NK activity.

journal_name

Surg Today

journal_title

Surgery today

authors

Sugimoto F,Tsukada K,Hatakeyama K,Yoshida K,Muto T

doi

10.1007/BF01636774

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

694-700

issue

8

eissn

0941-1291

issn

1436-2813

journal_volume

24

pub_type

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