Regulation of methionine adenosyltransferase activity by the glutathione level in rat liver during ischemia-reperfusion.

Abstract:

:Hepatic ischemia was induced by clamping the hepatic artery, portal vein, and bile duct. After 15 min of ischemia, the hepatic glutathione (GSH) content rapidly decreased. On the other hand, after the start of reperfusion, the hepatic GSH levels promptly increased and reached a peak at about 1 h, and thereafter decreased to a minimum level by 2 h. Under such conditions, we examined the changes in the methionine adenosyltransferase (MAT) activity in the liver. Though the time course of MAT activity was somewhat delayed compared with that of the hepatic GSH levels, both patterns were substantially similar during ischemia-reperfusion. In contrast to the changes in the MAT activity during ischemia-reperfusion, the levels of MAT protein were unchanged during these periods. When endogenous antioxidant coenzyme Q(10) (CoQ(10)) was administered to rats prior to ischemia, both the reduction in the MAT activity and hepatic GSH levels induced by ischemia-reperfusion were protected. Our findings suggest that CoQ(10) may posttranslationally regulate the MAT activity via the changes in the GSH level in the liver.

journal_name

Surg Today

journal_title

Surgery today

authors

Ito K,Miwa N,Hagiwara K,Yano T,Shimizu-Saito K,Goseki N,Iwai T,Horikawa S

doi

10.1007/PL00010035

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

1053-8

issue

10

eissn

0941-1291

issn

1436-2813

pii

10.1007/PL00010035

journal_volume

29

pub_type

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