Abstract：
:A series of joro spider toxins, novel polyamines sharing a common moiety of 2,4-dihydroxyphenylacetyl cadaverine, have been identified using various bioassays, such as inhibition of a glutamatergic transmission and insecticidal activity. In this paper, we tried to chemically find still unknown polyamine toxins in the venom of a joro spider, N. clavata, by several analytical methods based on the characteristics of the common moiety. An aqueous extract from 3000 venoms was separated by preparative high performance liquid chromatography (HPLC). The polyamine toxins were detected by monitoring the fluorescence produced in an on-line reaction of o-phthalaldehyde with amino groups and UV absorption of the phenol group. Two compounds in minute quantity were purified and analyzed by gas-liquid chromatography (GC) and HPLC, which we specifically developed for the simultaneous determination of amino acids and polyamines of the toxins. Judging from the constituents of the hydrolysate by GC and HPLC and the molecular weights determined by fast atom bombardment mass spectrometry, the two compounds were estimated to be N-(2,4-dihydroxyphenylacetyl-L-asparaginyl)-N'- (3-aminopropyl-beta-alanyl) cadaverine and N-(4-hydroxyphenylacetyl-L-asparaginyl)-N'-(3-aminopropyl-beta-ala nyl) cadaverine. These compounds were small in content and molecular weight compared with hitherto known toxins. Both were presumed to be biochemically primitive toxins and were named spidamine and joramine, respectively.
journal_name
Chem Pharm Bull (Tokyo)journal_title
Chemical & pharmaceutical bulletinauthors
Chiba T,Akizawa T,Matsukawa M,Pan-Hou H,Yoshioka Mdoi
10.1248/cpb.42.1864subject
Has Abstractpub_date
1994-09-01 00:00:00pages
1864-9issue
9eissn
0009-2363issn
1347-5223journal_volume
42pub_type
杂志文章abstract：:The objective of this study was to synthesize several new pregnane derivatives and evaluate them as antiandrogens. From the commercially available 16-dehydropregnenolone acetate (7), two new steroidal compounds were synthesized: 17alpha-hydroxy-17beta-methyl-16beta-phenyl-D-homoandrosta-1,4,6-triene-3,20-dione (18) an...
journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
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更新日期：2001-05-01 00:00:00
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章,评审
doi：10.1248/cpb.55.349
更新日期：2007-03-01 00:00:00
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.c16-00986
更新日期：2017-01-01 00:00:00
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.c15-00559
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.56.115
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.54.538
更新日期：2006-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1248/cpb.52.1302
更新日期：2004-11-01 00:00:00
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doi：10.1248/cpb.c17-00225
更新日期：2017-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1248/cpb.48.1458
更新日期：2000-10-01 00:00:00
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.40.1075
更新日期：1992-04-01 00:00:00
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.39.1883
更新日期：1991-07-01 00:00:00
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.39.2454
更新日期：1991-09-01 00:00:00
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.56.1276
更新日期：2008-09-01 00:00:00
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.38.2216
更新日期：1990-08-01 00:00:00
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.53.1515
更新日期：2005-12-01 00:00:00
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.43.2027
更新日期：1995-11-01 00:00:00
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.38.3249
更新日期：1990-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2011-01-01 00:00:00
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.39.3359
更新日期：1991-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：1991-06-01 00:00:00
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.49.203
更新日期：2001-02-01 00:00:00
abstract：:Investigation of an acetone extract of the leaves and twigs of Taxus sumatrana has resulted in the isolation of two new bicyclic taxoids, tasumatrols M (1), and N (2) and a new baccatin III derivative, tasumatrol O (3) together with the previous known 7-deacetylcanadensene (4) and 2alpha,7beta,13alpha-triacetoxy-5alph...
journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.53.808
更新日期：2005-07-01 00:00:00
abstract：:2-Acyl-tetrahydroisoquinoline-3-carboxylic acid derivatives were synthesized and biologically evaluated. (S)-2-(2,4-Hexadienoyl)-7-[2-(5-methyl-2-phenyloxazol-4-yl)ethoxy]-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid (14) showed peroxisome proliferator-activated receptor γ (PPARγ) and PPARα agonist activities and ...
journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.59.876
更新日期：2011-01-01 00:00:00
abstract：:The superoxide O2(·-) scavenging reaction of (+)-catechin (Cat), quercetin (Que), rutin, and α-tocopherol (α-TOH) as natural phenolic compounds is investigated on the basis of electrochemical and ESR spectral measurements with the aid of density functional theory (DFT) calculations. Reversibility of the O2/O2(·-) redo...
journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.c15-00447
更新日期：2015-01-01 00:00:00
abstract：:Anion-exchange resins modified with metal-porphine (M-Pr) have been investigated to develop a solid catalyst in the oxidative reaction of phenols by O2 in air. Co-Pr, which is easily prepared and separable from the reaction mixture, has been proved to accelerate the oxidative reaction of phenols such as 3,5-di-tertbut...
journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.48.1831
更新日期：2000-11-01 00:00:00
abstract：:Epidermal cells from newborn rat skin were cultured on type IV collagen-coated Millipore filter, and the transport of low density lipoprotein (LDL) labeled with Rhodamine B isothiocyanate (RB-LDL) through the cultured cell layer was examined. The transport of RB-LDL was dependent on temperature and biological energy. ...
journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.39.437
更新日期：1991-02-01 00:00:00
abstract：:Orally disintegrating tablets (ODTs) are useful for improving benefits for patients of various ages. Masking the unpleasant taste of a drug is an important factor in the compliance of patients who take ODTs. We evaluated the taste acceptability effects of various taste-masking methods on bitter famotidine ODTs as a cl...
journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章,随机对照试验
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更新日期：2012-01-01 00:00:00
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journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.56.1205
更新日期：2008-08-01 00:00:00
abstract：:A series of benzo[b]furan derivatives having a five-membered heterocyclic substituent at the 2-position were prepared from 2-(1-chloro-2-formylvinyl)benzo[b]furans (2) and 2-(4-alkylcarbamoylbuta-1,3-dienyl)benzo[b]furans. These 2-heterocyclic benzo[b]furans were evaluated for their cysteinyl leukotriene receptor (cys...
journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.c12-00560
更新日期：2012-01-01 00:00:00
abstract：:Fourteen flavonol glycosides including two new compounds were isolated from the leaves of two Diospyros plants (D. cathayensis and D. rhombifolia). The structures of isolated compounds were determined by spectroscopic analysis. The scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical of the isolated com...
journal_title：Chemical & pharmaceutical bulletin
pub_type： 杂志文章
doi：10.1248/cpb.53.591
更新日期：2005-05-01 00:00:00
