Vibrio metschnikovii, a rare cause of wound infection.

abstract：:Evidence of spotted fever group (SFG) rickettsiae was obtained from flea pools and individual ticks collected at three sites in northwestern Peru within the focus of an outbreak of febrile disease in humans attributed, in part, to SFG rickettsia infections. Molecular identification of the etiologic agents from these s...

journal_title：Journal of clinical microbiology

authors： Blair PJ,Jiang J,Schoeler GB,Moron C,Anaya E,Cespedes M,Cruz C,Felices V,Guevara C,Mendoza L,Villaseca P,Sumner JW,Richards AL,Olson JG

更新日期：2004-11-01 00:00:00

abstract：:Influenza epidemics arise through the accumulation of viral genetic changes, culminating in a novel antigenic type that is able to escape host immunity. Following an outbreak of the A/Fujian/411/2002-like strains in Asia, including China, Japan, and South Korea, in 2002, Australia and New Zealand experienced substanti...

journal_title：Journal of clinical microbiology

authors： Kang S,Yang IS,Lee JY,Park Y,Oh HB,Kang C,Kim KH

更新日期：2010-06-01 00:00:00

abstract：:We examined an antiserum agar method to study its reliability in screening Staphylococcus aureus strains for capsule production. The encapsulated S. aureus Smith diffuse strain was compared with its nonencapsulated variant, Smith compact, in CCY medium containing 0.5% NaCl and 5.0% Smith diffuse rabbit antiserum. A ha...

journal_title：Journal of clinical microbiology

authors： West TE,Apicella MA

更新日期：1984-08-01 00:00:00

abstract：:The extent of genetic and antigenic variation found in a population of human parainfluenza virus type 1 (HPIV-1) during a single local epidemic was investigated. Fifteen HPIV-1 strains isolated from children in 1991 were analyzed. Nucleotide sequence variation in the hemagglutinin-neuraminidase protein (HN) gene demon...

journal_title：Journal of clinical microbiology

authors： Henrickson KJ,Savatski LL

更新日期：1996-03-01 00:00:00

abstract：:A combined clinical and molecular epidemiologic analysis of 46 strains of Clostridium difficile, including 16 nosocomial isolates from one ward (outbreak ward) plus 17 other nosocomial isolates and 13 community-acquired isolates, was performed. HindIII digests of total cellular DNA were analyzed by restriction enzyme ...

journal_title：Journal of clinical microbiology

authors： Kristjánsson M,Samore MH,Gerding DN,DeGirolami PC,Bettin KM,Karchmer AW,Arbeit RD

更新日期：1994-08-01 00:00:00

abstract：:Of a total of 1043 macrolide-resistant Streptococcus pneumoniae isolates collected from 24 countries as part of PROTEKT 1999-2000, 71 isolates tested positive for both the mef(A) and erm(B) genes. Of 69 isolates subjected to further molecular investigations, all were resistant to tetracycline, 63 (91.3%) were resistan...

journal_title：Journal of clinical microbiology

authors： Farrell DJ,Morrissey I,Bakker S,Morris L,Buckridge S,Felmingham D

更新日期：2004-02-01 00:00:00

abstract：:Coronavirus disease (COVID) serological tests are essential to determine the overall seroprevalence of a population and to facilitate exposure estimates within that population. We performed a head-to-head assessment of enzyme immunoassays (EIAs) and point-of-care lateral flow assays (POCTs) to detect severe acute resp...

journal_title：Journal of clinical microbiology

authors： Charlton CL,Kanji JN,Johal K,Bailey A,Plitt SS,MacDonald C,Kunst A,Buss E,Burnes LE,Fonseca K,Berenger BM,Schnabl K,Hu J,Stokes W,Zelyas N,Tipples G

更新日期：2020-09-22 00:00:00

abstract：:The recovery of Mycobacterium tuberculosis from sputa positive or negative for acid-fast bacilli that were stored for 17 +/- 7 days and inoculated in the BACTEC MGIT 960 system (MGIT) was higher than that from sputa inoculated in Lowenstein-Jensen medium. MGIT is useful for isolation of M. tuberculosis from sputa subj...

journal_title：Journal of clinical microbiology

authors： Pardini M,Varaine F,Bonnet M,Orefici G,Oggioni MR,LONG-DRUG Study Group.,Fattorini L

更新日期：2007-02-01 00:00:00

abstract：:The Vidas Chlamydia test (CHL) is an automated enzyme-linked immunofluorescence assay for the detection of Chlamydia trachomatis. Positive and equivocal results are confirmed with a blocking assay. A mouse monoclonal antibody directed against the chlamydial lipopolysaccharides was used for the test. The CHL assay is w...

journal_title：Journal of clinical microbiology

authors： Schachter J,Jones RB,Butler RC,Rice B,Brooks D,Van der Pol B,Gray M,Moncada J

更新日期：1997-08-01 00:00:00

abstract：:Our point-prevalence survey followed an outbreak of methicillin-resistant Staphylococcus aureus (MRSA) in a long-term care facility and identified five MRSA strains, of which two possessed an outbreak genotype not encountered previously and three had another profile. All of them possessed SCCmec type V. Six methicilli...

journal_title：Journal of clinical microbiology

authors： Kerttula AM,Lyytikäinen O,Vuopio-Varkila J,Ibrahem S,Agthe N,Broas M,Jägerroos H,Virolainen A

更新日期：2005-12-01 00:00:00

abstract：:Fast and accurate identification and typing of pathogens are essential for effective surveillance and outbreak detection. The current routine procedure is based on a variety of techniques, making the procedure laborious, time-consuming, and expensive. With whole-genome sequencing (WGS) becoming cheaper, it has huge po...

journal_title：Journal of clinical microbiology

authors： Joensen KG,Scheutz F,Lund O,Hasman H,Kaas RS,Nielsen EM,Aarestrup FM

更新日期：2014-05-01 00:00:00

abstract：:We compared anaerobic lytic (AL) and pediatric aerobic resin-containing (Peds Plus/F) blood culture media for the isolation of Salmonella enterica serotype Typhi or Paratyphi A from children. The yields from AL and Peds Plus/F media were the same with equal volumes of blood, but recovery was faster from AL medium than...

journal_title：Journal of clinical microbiology

authors： Reller ME,Zaidi AK,Sultana S,Azeem S,Hanif B,Qureshi S,Hasan R,Bhutta Z,Akhter R,Goldmann DA

更新日期：2009-01-01 00:00:00

abstract：:Replica plating can be used for the detection of antibiotic resistance in normal flora. We have evaluated this application of the replica plating method by comparing it with a five-colony method. The replica plating method uses a single plate for each antibiotic, with a concentration just above that for borderline res...

journal_title：Journal of clinical microbiology

authors： Osterblad M,Leistevuo T,Huovinen P

更新日期：1995-12-01 00:00:00

abstract：:The L1 major capsid proteins of six human papillomavirus type 16 (HPV-16) strains were expressed in insect cells by using recombinant baculoviruses. Virus-like particles (VLPs) which appeared similar to empty virions were identified by electron microscopy for all HPV strains investigated. However, the yield of VLPs pr...

journal_title：Journal of clinical microbiology

authors： Touze A,El Mehdaoui S,Sizaret PY,Mougin C,Muñoz N,Coursaget P

更新日期：1998-07-01 00:00:00

abstract：:Inactivation of penicillin and gentamicin in cultures was achieved by using monoclonal antibodies against these antibiotics. A viridans group streptococcus (penicillin MIC, less than or equal to 0.06 micrograms/ml) and Escherichia coli ATCC 35218 (gentamicin MIC, less than or equal to 1 microgram/ml) were able to grow...

journal_title：Journal of clinical microbiology

authors： Sierra-Madero JG,Caulfield MJ,Hall GS,Washington JA

更新日期：1988-09-01 00:00:00

abstract：:The use of 1% unmodified rice starch and 1% horse serum instead of 2% soluble starch and 5% serum in Granada medium is described. These components result in a medium of increased stability, preventing spoilage after a few days of storage at room temperature. ...

journal_title：Journal of clinical microbiology

authors： Rosa-Fraile M,Rodríguez-Granger J,Camacho-Muñoz E,Sampedro A

更新日期：2005-04-01 00:00:00

abstract：:A simple enzyme-linked immunosorbent assay was developed for the identification of cultured mycobacteria belonging to the Mycobacterium tuberculosis complex, the Mycobacterium avium complex, and Mycobacterium kansasii. Six monoclonal antibodies were used: two (F23-49 and F24-2) were specific for the M. tuberculosis co...

journal_title：Journal of clinical microbiology

authors： Schöningh R,Verstijnen CP,Kuijper S,Kolk AH

更新日期：1990-04-01 00:00:00

abstract：:Type-specific antigen was purified from the supernatant of type III group B streptococcal cultures, tyrosylated, and bound to microtiter wells for an enzyme-linked immunosorbent assay. The immunological specificity of the antigen and the assay was shown by (i) reaction only with homologous unabsorbed rabbit sera and (...

journal_title：Journal of clinical microbiology

authors： Anthony BF,Concepcion NF,McGeary SA,Ward JI,Heiner DC,Shapshak P,Insel RA

更新日期：1982-08-01 00:00:00

abstract：:A total of 272 staphylococcal isolates from cases of bovine mastitis (159 Staphylococcus aureus) belonging to 12 different species were identified with ID32 STAPH galleries, and 51 of them were confirmed by 16S rRNA gene (rrs) sequencing. The same isolates were examined for their hemolytic activity on sheep blood agar...

journal_title：Journal of clinical microbiology

authors： Boerlin P,Kuhnert P,Hüssy D,Schaellibaum M

更新日期：2003-02-01 00:00:00

abstract：:Clostridium difficile is a Gram-positive bacterium commonly found in health care and long-term-care facilities and is the most common cause of antibiotic-associated diarrhea. Rapid detection of this bacterium can assist physicians in implementing contact precautions and appropriate antibiotic therapy in a timely manne...

journal_title：Journal of clinical microbiology

authors： Beck ET,Buchan BW,Riebe KM,Alkins BR,Pancholi P,Granato PA,Ledeboer NA

更新日期：2014-06-01 00:00:00

abstract：:Comparative studies on the detection of bovine serum immunoglobulin G antibodies to bluetongue virus with an enzyme-linked immunosorbent assay, an immunodiffusion method, and a serum neutralization assay demonstrated complete concordance between the enzyme-linked immunosorbent assay and the serum neutralization assay ...

journal_title：Journal of clinical microbiology

authors： Poli G,Stott J,Liu YS,Manning JS

更新日期：1982-01-01 00:00:00

abstract：:Campylobacter jejuni isolates from stool samples of patients with domestically acquired sporadic infections and from chicken from retail shops were studied during seasonal peaks from June to September over a 3-year period from 1996 to 1998. A large number of pulsed-field gel electrophoresis (PFGE) genotypes (a combine...

journal_title：Journal of clinical microbiology

authors： Hänninen ML,Perko-Mäkelä P,Pitkälä A,Rautelin H

更新日期：2000-05-01 00:00:00

abstract：:Identification of clinical isolates of Nocardia to the species level is important for defining the spectrum of disease produced by each species and for predicting antimicrobial susceptibility. We evaluated the usefulness of PCR amplification of a portion of the Nocardia 16S rRNA gene and subsequent restriction endonuc...

journal_title：Journal of clinical microbiology

authors： Conville PS,Fischer SH,Cartwright CP,Witebsky FG

更新日期：2000-01-01 00:00:00

abstract：:Salmonella isolates have traditionally been classified by serotyping, the serologic identification of two surface antigens, O-polysaccharide and flagellin protein. Serotyping has been of great value in understanding the epidemiology of Salmonella and investigating disease outbreaks; however, production and quality con...

journal_title：Journal of clinical microbiology

authors： McQuiston JR,Parrenas R,Ortiz-Rivera M,Gheesling L,Brenner F,Fields PI

更新日期：2004-05-01 00:00:00

abstract：:Clinical isolates of group B streptococci from body fluids and mucosal surfaces were examined for production of a trypsin-resistant antigen known as R protein. R protein was extracted with 1% trypsin from cells grown in a semidefined medium. The extracts were tested by immunodiffusion in agarose with a panel of antise...

journal_title：Journal of clinical microbiology

authors： Flores AE,Ferrieri P

更新日期：1989-05-01 00:00:00

abstract：:Buruli ulcer, an infection caused by Mycobacterium ulcerans, is, after tuberculosis and leprosy, the third most common mycobacterial disease. The mode of transmission of M. ulcerans is not exactly known, but since Buruli ulcer often occurs in focalized swampy areas, it is assumed that there is a reservoir of the patho...

journal_title：Journal of clinical microbiology

authors： Rondini S,Mensah-Quainoo E,Troll H,Bodmer T,Pluschke G

更新日期：2003-09-01 00:00:00

abstract：:The use of a new commercial separation column on whole test serum improved the method for detecting immunoglobulin M antibody to Epstein-Barr virus. The efficiency of this product in absorbing interfering immunoglobulin G was similar to that of another absorption product, staphylococcal protein A, but it has the advan...

journal_title：Journal of clinical microbiology

authors： Sumaya CV,Ench Y,Carrillo MA,Pope RM

更新日期：1984-08-01 00:00:00

abstract：:The pathogenesis of gastroduodenal diseases is related to the diversity of Helicobacter pylori strains. CagA-positive strains are more likely to cause gastric cancer than CagA-negative strains. Based on EPIYA (Glu-Pro-Ile-Tyr-Ala) motifs at the carboxyl terminus corresponding to phosphorylation sites, H. pylori CagA i...

journal_title：Journal of clinical microbiology

authors： Truong BX,Mai VT,Tanaka H,Ly le T,Thong TM,Hai HH,Van Long D,Furumatsu K,Yoshida M,Kutsumi H,Azuma T

更新日期：2009-12-01 00:00:00

abstract：:We describe enzyme immunoassays that use synthetic oligopeptides to discriminate serologically between human T-cell lymphotropic virus type I and II (HTLV-I and HTLV-II) infections. The peptides represented 20-amino acid segments between residues 111 and 130 (MA1) and residues 116 and 135 (MA2) of the p19 gag proteins...

journal_title：Journal of clinical microbiology

authors： Bonis J,Baillou A,Barin F,Verdier M,Janvier B,Denis F

更新日期：1993-06-01 00:00:00

abstract：:Although vaginitis caused by Saccharomyces cerevisiae is extremely rare, in recent years we have experienced an increasing frequency of S. cerevisiae isolation from the vaginas of fertile-age women. In order to investigate the epidemiology of these vaginal infections, a total of 40 isolates of S. cerevisiae derived fr...

journal_title：Journal of clinical microbiology

authors： Posteraro B,Sanguinetti M,D'Amore G,Masucci L,Morace G,Fadda G

更新日期：1999-07-01 00:00:00