Adrenal cortical cell transplantation.

Abstract:

PURPOSE:The adrenal cortex is a critical component of the hypothalamic-pituitary-adrenal/gonadal axis that coordinates the stress response and maintains homeostasis. The authors hypothesize that adrenal cortical cells can be transplanted in adrenal insufficiency states to regenerate the adrenal cortex. METHODS:Murine adrenal glands were dissociated into adrenal cortical cells. Cells cultured in a collagen matrix were transplanted under the renal capsule. The implants were retrieved 1, 2, 4, and 8 weeks later. Total RNA was extracted from the retrieved specimens and was analyzed by real-time polymerase chain reaction. RESULTS:All animals survived the surgical procedure. At implant retrieval, a distinct organoid could be visualized under the renal capsule. The expressions of adrenal-specific markers including Sf1, Dax1, Star, Cyp11a, Cyp11b1, and Cyp21 were detectable in the retrieved specimens up to 8 weeks posttransplantation. CONCLUSION:Primary adrenal cortical cells retained their gene expressions after heterotopic transplantation. Ex vivo gene transfer followed by adrenal cortical cell transplantation may lead to curative therapy for patients with adrenal insufficiency.

journal_name

J Pediatr Surg

authors

Dunn JC,Chu Y,Lam MM,Wu BM,Atkinson JB,McCabe ER

doi

10.1016/j.jpedsurg.2004.08.006

subject

Has Abstract

pub_date

2004-12-01 00:00:00

pages

1856-8

issue

12

eissn

0022-3468

issn

1531-5037

pii

S0022346804005512

journal_volume

39

pub_type

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