Abstract:
:The daily production of melatonin from the pineal gland influences circadian and seasonal behaviour and physiology. To further understand how melatonin may function, it is important to characterize the receptor and signal transduction systems. Using the detergent digitonin, we were able to solubilize the receptor from the ovine pars tuberalis (PT) membrane. The receptor was isolated as a complex associated with its heterotrimeric G-protein. In the solubilized state, pre-bound 125I-2-iodomelatonin was stable at 4 degrees C, but was displaceable by GTP gamma S. The receptor-G-protein complex could be separated by molecular mass using native polyacrylamide gel electrophoresis. We demonstrate that the receptor-complex has a molecular mass of 525 kDa and differs from solubilized receptor-complexes isolated from either the lizard brain, chicken brain or the ovine hippocampus. Furthermore the receptor complex isolated from the hippocampus had the lowest molecular mass of these tissues (365 kDa) and was found not to be sensitive to GTP gamma S. This may indicate the existence of a distinct non-G-protein coupled form of the receptor.
journal_name
J Neuroendocrinoljournal_title
Journal of neuroendocrinologyauthors
Barrett P,MacLean A,Morgan PJdoi
10.1111/j.1365-2826.1994.tb00613.xsubject
Has Abstractpub_date
1994-10-01 00:00:00pages
509-15issue
5eissn
0953-8194issn
1365-2826journal_volume
6pub_type
杂志文章abstract::The paraventricular nucleus (PVN) in the hypothalamus receives inputs from the hippocampus The present study explored the influence of the hippocampus on genes mediating glucocorticoid feedback in the PVN. Accordingly, the expression of mRNAs for corticotrophin-releasing hormone (CRH), the mineralocorticoid receptor (...
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pub_type: 杂志文章,评审
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journal_title:Journal of neuroendocrinology
pub_type: 杂志文章
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journal_title:Journal of neuroendocrinology
pub_type: 杂志文章
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journal_title:Journal of neuroendocrinology
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journal_title:Journal of neuroendocrinology
pub_type: 杂志文章
doi:10.1111/jne.12556
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journal_title:Journal of neuroendocrinology
pub_type: 杂志文章
doi:10.1111/j.1365-2826.1990.tb00395.x
更新日期:1990-02-01 00:00:00
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journal_title:Journal of neuroendocrinology
pub_type: 杂志文章
doi:10.1046/j.1365-2826.1996.04835.x
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journal_title:Journal of neuroendocrinology
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Journal of neuroendocrinology
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更新日期:2005-11-01 00:00:00
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journal_title:Journal of neuroendocrinology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2826.2009.01837.x
更新日期:2009-03-01 00:00:00
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journal_title:Journal of neuroendocrinology
pub_type: 杂志文章
doi:10.1111/j.1365-2826.2005.01358.x
更新日期:2005-10-01 00:00:00
abstract::The anteroventral periventricular nucleus (AVPV) is sexually dimorphic, presenting a higher neuronal density in females. The AVPV contains a dense collection of oestrogen and progesterone receptors and has been related to the modulation of gonadotrophin-releasing hormone (GnRH) secretion and gene expression in respons...
journal_title:Journal of neuroendocrinology
pub_type: 杂志文章
doi:10.1111/j.1365-2826.2006.01457.x
更新日期:2006-09-01 00:00:00