Abstract：
OBJECTIVE:To estimate the effect of protein supplementation of culture media on reactive oxygen species production and incidence of apoptosis in preimplantation mouse embryos. METHODS:A total of 72 two-cell mouse embryos were cultured in human tubal fluid (HTF) alone (HTF-alone, control) and 71 embryos in HTF with protein supplementation (10% serum substitute supplement; HTF-SSS) for 72 hours. Total cell number per embryo was determined by staining with Hoechst 33258. Allocation of inner cell mass and trophectoderm in blastocysts and incidence of apoptosis were determined by confocal microscopy. Levels of reactive oxygen species in culture media were measured by chemiluminescence assay using luminol as probe. RESULTS:Blastocyst development, total cell number, and the inner cell mass/trophectoderm ratio were similar between the 2 groups. The blastocyst hatching rate was significantly higher in the HTF-SSS group than in the HTF-alone group (20% compared with 4%, P = .007). Level of reactive oxygen species was significantly higher in HTF-alone compared with HTF-SSS at 24 hours (median and interquartile range 28 [13, 43] compared with 0 [0, 1], P = .02), 48 hours (24 [21, 26] compared with 2 [1, 2], P = .02), and 72 hours (26 [9, 32] compared with 2 [2, 3], P = .03). The incidence of apoptosis in blastocysts cultured in HTF-SSS was significantly lower than those in HTF-alone group (mean +/- standard deviation 2.38 +/- 0.68 and 5.81 +/- 1.11, respectively, P = .001). CONCLUSION:Protein supplementation of culture media improves the hatching rate and reduces reactive oxygen species levels and the incidence of apoptosis in mouse preimplantation embryos.
journal_name
Obstet Gynecoljournal_title
Obstetrics and gynecologyauthors
Esfandiari N,Falcone T,Agarwal A,Attaran M,Nelson DR,Sharma RKdoi
10.1097/01.AOG.0000152384.91385.71subject
Has Abstractpub_date
2005-03-01 00:00:00pages
653-60issue
3eissn
0029-7844issn
1873-233Xpii
105/3/653journal_volume
105pub_type
杂志文章abstract：OBJECTIVE:To identify the effects of percutaneous estradiol (E2) implants on bone histology and bone mass of postmenopausal women with low bone mineral density. METHODS:Sixteen postmenopausal women with low bone mineral density were treated with 75-mg E2 implants. Each had iliac crest bone biopsies performed, followin...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:Six girls with suspected Rokitansky syndrome underwent magnetic resonance imaging (MRI) and then sonography and laparoscopy to confirm the clinical diagnosis and define the precise characteristics of the malformation. The uterus was missing in all; four had müllerian rudiments in place of the uterus, which were cavita...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：OBJECTIVE:We sought to investigate the prevalence and risk factors for vaginal yeast colonization over a 1-year period. METHODS:We conducted a longitudinal cohort study of 1,248 asymptomatic young women by collecting demographic and behavioral data at baseline, 4, 8, and 12 months. RESULTS:Seventy percent of women we...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：10.1097/01.AOG.0000140687.51048.73
更新日期：2004-11-01 00:00:00
abstract：:This is a case report of a woman having an IUD which became partially extruded through the uterine wall and went undetected until the patient became pregnant. Severe metritis followed premature rupture of the membranes during the early part of the third trimester. Cesarean section hysterectomy led to a salutary outc...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1969-12-01 00:00:00
abstract：OBJECTIVE:To compare risk of reintervention, long-term clinical outcomes, and health care utilization among women who have bulk symptoms from leiomyoma and who underwent the following procedures: hysterectomy, myomectomy, uterine artery embolization, and magnetic resonance-guided, focused ultrasound surgery. METHODS:T...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：10.1097/AOG.0000000000002331
更新日期：2017-11-01 00:00:00
abstract：OBJECTIVE:To identify the top-rated pregnancy wheel applications (apps) using a newly developed APPLICATIONS scoring system. METHODS:A list of pregnancy wheel apps was identified. Consumer-based and inaccurate apps were excluded. The APPLICATIONS scoring system was developed to rate the remaining apps. Application com...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：10.1097/AOG.0000000000000842
更新日期：2015-06-01 00:00:00
abstract：OBJECTIVE:To analyze the influence of body mass on the outcome of surgery for urinary incontinence. METHODS:Among 291 women operated on for stress incontinence, 187 (64%) were available for follow-up at 5 years. Eighty women had anterior colporrhaphy, 49 anterior colporrhaphy with needle suspension of the bladder neck...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0029-7844(98)00513-4
更新日期：1999-05-01 00:00:00
abstract：OBJECTIVE:To assess the efficacy of postural changes, such as crossing the legs, in reducing urine leakage in women with stress urinary incontinence. METHODS:Sixty-five women with urodynamically proven stress incontinence underwent a series of standing cough stress tests in each of four postures: standing, crossing th...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：OBJECTIVE:To evaluate whether prophylactic dronabinol, a synthetic tetrahydrocannabinol, reduces pain during medical abortion. METHODS:We conducted a randomized, double-blind, placebo-controlled trial of women undergoing medical abortion with mifepristone and misoprostol up through 70 days of gestation. All participan...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章,随机对照试验
doi：10.1097/AOG.0000000000003850
更新日期：2020-06-01 00:00:00
abstract：:This report describes an innovative use of videography in teaching retropubic space anatomy and surgery. After the standard abdominal incision has been made, the second surgical assistant places a gynecologic laparoscope, fitted with an endoscopic camera, into the retropubic space and guides the laparoscope documentin...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：OBJECTIVE:To estimate the effectiveness of a behavioral educational intervention to reduce postpartum depressive symptoms among minority mothers. METHODS:We recruited 540 self-identified black and Latina mothers during their postpartum hospital stay and randomized them to receive a behavioral educational intervention ...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章,随机对照试验
doi：10.1097/AOG.0b013e318250ba48
更新日期：2012-05-01 00:00:00
abstract：:We reviewed our experience with a policy of revising the estimated probability of an open neural tube defect for those cases in which maternal serum alpha-fetoprotein (MSAFP) was elevated and the ultrasound examination was normal. Assuming a sensitivity for the ultrasound diagnosis of neural tube defects of 90%, the r...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：:The inability to make a definitive diagnosis in the fetus with a sonographically identified abnormality often results in parental and physician uncertainty. An antenatal chromosome evaluation could resolve this uncertainty. Forty-one fetuses with an abnormal ultrasound examination were tested for karyotypic abnormalit...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:The reported frequency of alcohol abuse during pregnancy was studied in a large urban public hospital in Dallas, Texas. During 1977-1980, 5602 pregnant women were surveyed and 0.7% (95% confidence interval 0.6-0.8%) reported abusing alcohol during pregnancy. In 1987, 1.4% (95% confidence interval 1.3-1.5%) of 1032 pre...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：OBJECTIVE:To establish the optimum oral daily dose of micronized medroxyprogesterone acetate, given in combination with a fixed oral dose of estrone (E1) sulfate as hormone replacement therapy, that provides endometrial protection and induces cessation of vaginal bleeding. METHODS:This multicenter, randomized, double-...
journal_title：Obstetrics and gynecology
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0029-7844(98)00038-6
更新日期：1998-05-01 00:00:00
abstract：:Although urine cultures are performed routinely in managing acute pyelonephritis in pregnancy, little is known about the clinical usefulness of these cultures. The same is true for blood cultures. To evaluate their role in managing patients, we reviewed the records of 156 cases to determine to what extent such culture...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：:A thorough inspection of the pelvis for adhesions was made at the time of laparoscopic tubal sterilization in 2131 patients. Contraceptive use was then compared in women with adhesions (cases) and those without adhesions (controls). Current or previous intrauterine device (IUD) use was not associated with a statistica...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：OBJECTIVE:To examine the effect of persistent occiput posterior position on neonatal outcome. METHODS:This is a retrospective cohort study of 31,392 term, cephalic, singleton births. Women with neonates born in persistent occiput posterior position at delivery were compared to those with occiput anterior position. Wom...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：10.1097/01.AOG.0000206217.07883.a2
更新日期：2006-04-01 00:00:00
abstract：:A woman in her 34th week of gestation required emergency appendectomy for perforation, with postoperative wound dehiscence secondary to fasciitis and tension on the suture line. Polypropylene mesh was used to repair the abdominal wall. Fetal distress necessitated a cesarean section. The mother and child both survived ...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:Treatment of neonatal mice with diethylstilbestrol results in the appearance of adenosis in the uterine cervix and upper part of the vagina in later life. Using dry mount autoradiography, the uptake and distribution of [3H]-estradiol in the same regions were studied after in vivo injections with [3H]-estradiol. It was...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：10.1097/00006250-197804000-00016
更新日期：1978-04-01 00:00:00
abstract：OBJECTIVE:To estimate the risk for nodal metastasis in women with endometrial cancer based on uterine characteristics on pathology. METHODS:From a study of staging for uterine cancer, women were identified as being at low risk for nodal metastasis based on three specific criteria on final pathology reports: 1) less th...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：10.1097/AOG.0b013e318240de51
更新日期：2012-02-01 00:00:00
abstract：OBJECTIVE:To evaluate a possible correlation between a new variable-persistent breech presentation-and the success rate of external cephalic version (ECV). METHODS:This was a retrospective study of ECVs performed from January 2008 through January 2019 in an Israeli tertiary care hospital. The study group included all ...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：10.1097/AOG.0000000000004231
更新日期：2021-02-01 00:00:00
abstract：:The incidence of diverticular disease of the colon has increased during the course of this century. The management of diverticulitis has changed in many centers from a conservative medical approach to a more aggressive surgical one. The surgical technique has also evolved; primary resection and anastomosis is currentl...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1981-11-01 00:00:00
abstract：:In some parts of Dade County, Florida, perinatal mortality rates have revealed serious problems in the delivery of health care to poor pregnant women. From 1982-1985, the reported perinatal mortality rates varied from 32-36 per 1000 live births, more than double the national average. Under the leadership of the Primar...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章,收录出版
doi：
更新日期：1991-09-01 00:00:00
abstract：:Twenty-one subjects with pregnancy-induced hypertension were investigated with regard to the relationship between maternal hemodynamics and fetal growth. Five of the infants were small for gestational age (SGA) (less than tenth percentile) and 16 were appropriate for gestational age (AGA) (greater than tenth percentil...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：:Fetal movements (FM) were measured using a real-time B-scan method in 50 women in the third trimester. One hundred ninety-five observations were made. The number of FM per 20-minute observation period was similar in normal, diabetic, and hypertensive pregnancies and in patients with placenta previa, but was significan...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1979-12-01 00:00:00
abstract：OBJECTIVE:To evaluate the complications of late second-trimester abortions (18-22 weeks) by laminaria dilation and evacuation, and the obstetric outcome of subsequent pregnancies. METHODS:Dilation of the cervix was achieved by repeated laminaria tent replacement. Evacuation was carried out in the outpatient clinic usi...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0029-7844(96)00170-6
更新日期：1996-09-01 00:00:00
abstract：BACKGROUND:A rare type of placentation leading to cesarean delivery and hysterectomy is described. CASE:A young multigravida in the 26th week of gestation was referred to our department with a history of vaginal bleeding and suspected placenta previa. Three previous children were delivered by elective cesarean. Ultras...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0029-7844(03)00629-x
更新日期：2003-09-01 00:00:00
abstract：OBJECTIVE:To examine preterm births among African-American and Hispanic women who delivered at an inner-city public hospital in the context of contemporaneously increasing rates in the United States. METHODS:The rates of preterm birth, defined as birth before 37 weeks of gestation, for singleton neonates with birth we...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：10.1097/AOG.0b013e318195e257
更新日期：2009-03-01 00:00:00
abstract：OBJECTIVE:To determine whether obstetric admissions to the intensive care unit (ICU) are useful quality-assurance indicators. METHODS:We analyzed retrospectively obstetric ICU admissions at two tertiary care centers from 1991 to 1997. RESULTS:The 131 obstetric admissions represented 0.3% of all deliveries. The majori...
journal_title：Obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0029-7844(99)00274-4
更新日期：1999-08-01 00:00:00
