Abstract:
:The present study investigated the effect of adrenomedullin, a novel vasorelaxant peptide, on the migration of cultured rat vascular smooth muscle cells (SMCs) by using the Boyden-chamber method. Fetal calf serum (FCS) and platelet-derived growth factor (PDGF)-BB strongly stimulated SMC migration. Adrenomedullin clearly inhibited SMC migration stimulated with 5% and 10% FCS in a concentration-dependent manner. The migration induced by 10 and 25 ng/mL PDGF-BB was also inhibited by adrenomedullin in a concentration-dependent manner. Inhibition by adrenomedullin of FCS- and PDGF-induced SMC migration was paralleled by an increase in the cellular level of cAMP. In fact, the percent increase in cAMP level was strongly correlated with the percent decrease in migration activity of SMCs after treatment with adrenomedullin. 8-Bromo cAMP, a cAMP analogue, reproduced the inhibition by adrenomedullin of FCS- and PDGF-induced SMC migration. An activator of adenylate cyclase, forskolin, also reduced FCS- and PDGF-induced SMC migration. These data indicate that adrenomedullin inhibits the migration of SMCs stimulated with FCS and PDGF, probably through a cAMP-dependent process. On the basis of these results and the finding that adrenomedullin is synthesized in and secreted from vascular endothelial cells, adrenomedullin may play a role as a local antimigration factor in some pathophysiological states.
journal_name
Circ Resjournal_title
Circulation researchauthors
Horio T,Kohno M,Kano H,Ikeda M,Yasunari K,Yokokawa K,Minami M,Takeda Tdoi
10.1161/01.res.77.4.660subject
Has Abstractpub_date
1995-10-01 00:00:00pages
660-4issue
4eissn
0009-7330issn
1524-4571journal_volume
77pub_type
杂志文章abstract::Heptanol blocks sodium current (INa) in nerve, but its effects on cardiac INa have not been well characterized. Block of INa by heptanol was studied in 16 internally perfused voltage-clamped cardiac Purkinje cells at reduced Na+ (45 mM outside, 0 mM inside). Heptanol block of peak sodium conductance was well described...
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pub_type: 杂志文章
doi:10.1161/01.res.68.4.977
更新日期:1991-04-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章,评审
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更新日期:2009-09-11 00:00:00
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pub_type: 杂志文章
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更新日期:1989-03-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.119.316075
更新日期:2020-03-27 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.110.217570
更新日期:2010-07-23 00:00:00
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doi:10.1161/01.res.84.11.1237
更新日期:1999-06-11 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.112.265678
更新日期:2012-09-14 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.78.3.415
更新日期:1996-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.RES.0000240147.49390.61
更新日期:2006-09-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.111.240150
更新日期:2011-09-30 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.62.6.1111
更新日期:1988-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.55.3.295
更新日期:1984-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.83.6.644
更新日期:1998-09-21 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000104084.88317.91
更新日期:2003-11-28 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.78.2.262
更新日期:1996-02-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.59.3.270
更新日期:1986-09-01 00:00:00
abstract::High sodium intake (HNa) increases brain ouabainlike activity (OLA) in rats. In spontaneously hypertensive rats (SHR), HNa exaggerates development of hypertension and pressor and sympathoexcitatory responses to stress. To investigate whether dietary sodium-induced changes in brain OLA play a functional role, responses...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.70.2.430
更新日期:1992-02-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.77.2.239
更新日期:1995-08-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:1998-02-23 00:00:00
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pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000061571.49375.E1
更新日期:2003-03-21 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.70.5.893
更新日期:1992-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.50.2.294
更新日期:1982-02-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2019-07-19 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.87.11.1040
更新日期:2000-11-24 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.60.4.626
更新日期:1987-04-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.68.3.683
更新日期:1991-03-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.84.4.371
更新日期:1999-03-05 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/CIRCRESAHA.110.216523
更新日期:2010-05-28 00:00:00