Abstract：
:Phenylethanolamine-N-methyltransferase (PNMT) catalyzes the conversion of norepinephrine to epinephrine. The enzyme, obtained from bovine adrenal gland, was incubated with PbCl2 at 23 degrees for various times prior to assay at 37 degrees. Inhibition developed slowly and reached a maximum after 45 min. In the presence of 4.5 nmoles PbCl2 (15 microM), 5.8 microgram protein was inhibited 50%, and inhibition was complete at 18 nmoles PbCl2 (60 microM). At maximum inhibition the PbCl2: protein ratio was 3.1 nmoles PbCl2/microgram protein. In the presence of PbCl2, the graph of enzyme activity versus protein concentration intercepted the abscissa to the right of the origin, indicating that lead is an irreversible or very slowly reversible inhibitor. The activity of PNMT which had been exposed to PbCl2 (2.6 or 5.2 nmoles PbCl2/microgram protein) was not restored by the addition of EDTA, DL-penicillamine or 1,3-dithiothreitol even when the concentration of the chelator was in 10 to 200-fold mole excess over PbCl2. DL-Penicillamine and 1,3-dithiothreitol were unable to prevent PbCl2 inhibition of the enzyme when combined with PbCl2 1 hr before addition of enzyme. EDTA could prevent 40% of PbCl2 inhibition but a decrease in total enzyme activity was noted in the presence of this chelator. Dialysis of the PbCl2-inhibited enzyme against buffer alone or buffer plus DL-penicillamine did not result in restoration of PNMT activity.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Caspers MLdoi
10.1016/0006-2952(82)90409-9subject
Has Abstractpub_date
1982-06-01 00:00:00pages
1985-8issue
11eissn
0006-2952issn
1873-2968pii
0006-2952(82)90409-9journal_volume
31pub_type
杂志文章abstract：:Glucose metabolism and hemoglobin reactivity in intact human erythrocytes were assessed in the presence of the tryptophan metabolites, 3-hydroxyanthranilate (3-HAT), quinolinate and picolinate. Of these compounds, only 3-HAT altered red cell oxidative status by inducing, in a dose-dependent manner, formation of methem...
journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(89)90301-8
更新日期：1989-05-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：2016-04-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：2014-08-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2017.03.003
更新日期：2017-05-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(87)90391-1
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2009.07.002
更新日期：2009-11-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(95)00265-2
更新日期：1995-10-12 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2005.04.037
更新日期：2005-08-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：2018-08-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(94)90343-3
更新日期：1994-04-20 00:00:00
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pub_type： 杂志文章
doi：10.1016/0006-2952(95)00108-c
更新日期：1995-06-29 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(86)90318-7
更新日期：1986-05-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(92)90156-d
更新日期：1992-06-23 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(92)90153-a
更新日期：1992-06-23 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(94)90015-9
更新日期：1994-01-20 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(87)90644-7
更新日期：1987-10-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/s0006-2952(95)02230-9
更新日期：1996-03-08 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2016.02.015
更新日期：2016-05-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(86)90370-9
更新日期：1986-02-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章,评审
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更新日期：2010-12-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2006.10.002
更新日期：2007-01-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2010.06.027
更新日期：2010-10-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2008.03.015
更新日期：2008-06-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(83)90455-0
更新日期：1983-04-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2019.04.015
更新日期：2019-06-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2011.12.003
更新日期：2012-03-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2015.12.004
更新日期：2016-02-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2010.01.034
更新日期：2010-06-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(83)90329-5
更新日期：1983-05-15 00:00:00
abstract：:In an effort to identify the human cytochromes P450 involved in the N-demethylation of dextromethorphan, the kinetics of 3-methoxymorphinan formation were studied in microsomal enzyme systems. Under initial rate conditions, 3-methoxymorphinan formation demonstrated single enzyme Michaelis-Menten kinetics using microso...
journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(94)90237-2
更新日期：1994-07-05 00:00:00
