当前位置： SCI文献检索 > RADIOLOGY期刊下所有文献 > CT perfusion for determination of pharmacologically mediated blood flow changes in an animal tumor model.

CT perfusion for determination of pharmacologically mediated blood flow changes in an animal tumor model.

Abstract：

PURPOSE:To prospectively compare single- and multisection computed tomographic (CT) perfusion for tumor blood flow determination in an animal model. MATERIALS AND METHODS:All animal protocols and experiments were approved by the institutional animal care and use committee before the study was initiated. R3230 mammary adenocarcinoma was implanted in 11 rats. Tumors (18-20 mm) were scanned with dynamic 16-section CT at baseline and after administration of arsenic trioxide, which is known to cause acute reduction in blood flow. The concentration of arsenic was titrated (0-6 mg of arsenic per kilogram of body weight) to achieve a defined blood flow reduction (0%-75%) from baseline levels at 60 minutes, as determined with correlative laser Doppler flowmetry. The mean blood flow was calculated for each of four 5-mm sections that covered the entire tumor, as well as for the entire tumor after multiple sections were processed. Measurements obtained with both methods were correlated with laser Doppler flowmetry measurements. Interobserver agreement was determined for two blinded radiologists, who calculated the percentage of blood flow reduction for the "most representative" single sections at baseline and after arsenic administration. These results were compared with the interobserver variability of the same radiologists obtained by summing blood flow changes for the entire tumor volume. RESULTS:Overall correlations for acute blood flow reduction were demonstrated between laser Doppler flowmetry and the two CT perfusion approaches (single-section CT, r=0.85 and r(2)=0.73; multisection CT, r=0.93 and r(2)=0.87; pooled data, P=.01). CT perfusion disclosed marked heterogeneity of blood flow, with variations of 36% +/- 13 between adjacent 5-mm sections. Given these marked differences, interobserver agreement was much lower for single-section CT (standard deviation, 0.22) than for multisection CT (standard deviation, 0.10; P=.01). CONCLUSION:Multisection CT perfusion techniques may provide an accurate and more reproducible method of tumor perfusion surveillance than comparison of single representative tumor sections.

journal_name

Radiology

journal_title

Radiology

authors

Hakimé A,Peddi H,Hines-Peralta AU,Wilcox CJ,Kruskal J,Lin S,de Baere T,Raptopoulos VD,Goldberg SN

doi

10.1148/radiol.2433052048

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

712-9

issue

eissn

0033-8419

issn

1527-1315

pii

243/3/712

journal_volume

243

pub_type

杂志文章

在线工具

CT perfusion for determination of pharmacologically mediated blood flow changes in an animal tumor model.