Abstract:
OBJECTIVE:To analyze the correlation between the genotypic and phenotypic patterns of p53 in patients with transitional cell carcinoma (TCC) of the urinary bladder. MATERIALS AND METHODS:Cross-sectional study of 73 patients diagnosed with TCC. DNA was obtained from the tumor tissue to perform polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) of exons 5-9 of the p53 gene, with automatic sequencing done on any mutated samples. Immunohistochemistry (IHC) was also performed using anti-human P53 monoclonal antibody, and the diagnostic performance of this test was analyzed by a ROC curve, using the presence of p53 mutations found by PCR-SSCP as 'gold standard'. RESULTS:The cutoff point for defining immunopositivity was 20%. IHC had a specificity of 62.9%, and a sensitivity of 65.8%. The highest sensitivity values appeared in G3 tumors (75%) and infiltrating tumors (71.4%), and the highest specificity values were observed in G1 (77.7%) and G2 tumors (90%) and superficial tumors (66.6%). Mutations in exon 8 gave a positive result most frequently (73.7%) and were considered most relevant in terms of altering P53 function (60.9%). False negatives were documented in 28.5% of infiltrating tumors, and false positives in 33.4% of superficial tumors. CONCLUSIONS:There is a moderate correlation between p53 mutations and P53 protein overexpression, with this stronger in high-grade, infiltrating tumors, in exon 8 mutations, and when the mutation induces relevant changes in the protein structure. Although IHC is useful in routine clinical practice, the classic prognostic factors should still be considered the most important in the follow-up of these patients.
journal_name
Urol Intjournal_title
Urologia internationalisauthors
Salinas-Sánchez AS,Atienzar-Tobarra M,Lorenzo-Romero JG,Sánchez-Sánchez F,Giménez-Bachs JM,Donate-Moreno MJ,Pastor-Navarro H,Hernández-Millán I,Segura-Martín M,Escribano-Martínez Jdoi
10.1159/000109717subject
Has Abstractpub_date
2007-01-01 00:00:00pages
321-7issue
4eissn
0042-1138issn
1423-0399pii
000109717journal_volume
79pub_type
杂志文章abstract:OBJECTIVES:To assess the role of color Doppler flow imaging (CDFI) in the evaluation of Chinese patients with erectile dysfunction (ED) and to explore the association between penile rigidity and hemodynamic parameters of CDFI. METHODS:Totally 74 ED patients with an age range of 22-69 (median age 47) were included in o...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000127350
更新日期:2008-01-01 00:00:00
abstract::We injected 20 micrograms of prostaglandin E1 (PGE1) intracavernously in 32 patients with impotence, we then examined penile blood flow using a Xe-133 washout technique. In addition, we also performed a cavernosography. After PGE1 injection, 18 patients (56%) showed complete erection, 10 (31%) tumescence, and 4 (13%) ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282131
更新日期:1991-01-01 00:00:00
abstract::Calcium oxalate kidney stone formers are invariably advised to increase their fluid intake. In addition, magnesium therapy is often administered. Recently, a prospective study showed that a high dietary intake of calcium reduces the risk of symptomatic kidney stones. The present study was performed to test whether sim...
journal_title:Urologia internationalis
pub_type: 临床试验,杂志文章
doi:10.1159/000282958
更新日期:1997-01-01 00:00:00
abstract::The authors improved the Nélaton catheter with a pair of carbon fiber electrodes as previously published and also improved the Foley catheter with a pair of carbon fiber electrodes which are less painful and easier to use. We also have made efforts how to record the vesical electromyogram (EMG) simultaneously with cys...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000280979
更新日期:1984-01-01 00:00:00
abstract::The prevalence of Chlamydia trachomatis and other microbes was studied in 94 semen samples from asymptomatic infertile males. Simultaneously, we sought evidence for inflammation of the genital tract by determining the polymorphonuclear granulocyte (PMN)-elastase concentration in the seminal plasma. The C. trachomatis ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282675
更新日期:1994-01-01 00:00:00
abstract::We report a case of a 75-year-old female with pheochromocytoma of the bladder. Clinical evaluation included ultrasonography, intravenous pyelography, CT scan, I-MIBG scintiscan. A transurethral resection was performed for a exophytic tumor of 2 cm diameter. The histological result indicated the diagnosis of bladder ph...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000064868
更新日期:2000-01-01 00:00:00
abstract::Ninety-five patients with erectile dysfunction underwent standardized cavernosometry and cavernosography. In a first stage, the investigation was performed in each patient without and in a second stage with 60 mg papaverine injected intracavernously. A pathological maintenance flow rate between 20 and 120 ml/min decre...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282389
更新日期:1992-01-01 00:00:00
abstract::Bisphosphonates are a standard treatment for metastatic bone disease, although agents vary in their potential for renal toxicity. This case report describes the reversal of zoledronic acid-induced renal toxicity by switching treatment to ibandronate in a patient with hormone-refractory prostate cancer and bone metasta...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000132707
更新日期:2008-01-01 00:00:00
abstract::The anterior nutcracker syndrome is defined by the compression of the left renal vein between the aorta and superior mesenteric artery, usually related to the occurrence of hematuria. We report the case of an uncommon complication of the nutcracker syndrome. A 75-year-old woman was referred to our institution for left...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000381270
更新日期:2016-01-01 00:00:00
abstract:INTRODUCTION:The aims of this study were to establish the prevalence of enuresis, to determine accompanying factors, and to identify common methods in the management of enuresis in Turkish children aged between 6 and 11 years living in eastern Anatolia. METHODS:The parents of 3,527 schoolchildren aged between 6 and 11...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000079696
更新日期:2004-01-01 00:00:00
abstract::We report the case of a woman who presented with obstruction and urosepsis of her left kidney secondary to small cystine calculi. The calculi could not be seen on initial plain abdominal X-ray. However, following percutaneous nephrostogram the calculi became more radio-dense and visible on later X-rays. Experimental e...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000030375
更新日期:1999-01-01 00:00:00
abstract::It is well known that diabetes mellitus is accompanied by complications of sexual dysfunction and it is believed that diabetic neuropathy may cause impotence. In our study, we found that not all the patients who visited our center with the chief complaint of diabetic impotence were suffering from organic impotence, an...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282978
更新日期:1997-01-01 00:00:00
abstract:INTRODUCTION:The use of cadaveric pediatric kidneys has been suggested as a means to overcome organ shortage, but is debated because of technical complications and an increased incidence of functional allograft impairment. METHODS:We experienced 2 cases of cadaveric renal transplantation from a non-heart-beating pedia...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000068778
更新日期:2003-01-01 00:00:00
abstract:INTRODUCTION:We aimed to evaluate the predictive factors in a holistic manner for ureterorenoscopy (URS) outcomes in proximal ureteral stones by a multicenter study. MATERIALS AND METHODS:The data of patients who underwent URS for proximal ureteral stones between the years 2015 and 2018 in eight centers were recorded ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000504790
更新日期:2020-01-01 00:00:00
abstract::By transforming uroflowmetrograms from curves of flow rate versus time into curves of flow rate versus instantaneous bladder volume, additional information about the variation of the flow rate during voiding is obtained. These curves show that, within a certain volume range, the maximum flow rate is more or less indep...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000280157
更新日期:1977-01-01 00:00:00
abstract::A review of the recent literature on the surgical management of anterior urethral stricture was performed. The literature was searched via PubMed using the search terms 'urethral stricture' and 'urethroplasty' from 1996 to 2009. The management of anterior urethral strictures is changing rapidly in the sense that the r...
journal_title:Urologia internationalis
pub_type: 杂志文章,评审
doi:10.1159/000319501
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVES:This meta-analysis was to assess solifenacin and tolterodine in patients with overactive bladder. METHODS:We searched PubMed, the Cochrane Library, EMBASE, CNKI, Wanfang, and ClinicalTrials.gov databases for randomized controlled trials (RCTs). The efficacy endpoint was daily micturition frequency, daily ur...
journal_title:Urologia internationalis
pub_type: 杂志文章,meta分析
doi:10.1159/000497311
更新日期:2019-01-01 00:00:00
abstract::Nuclear Magnetic Resonance (NMR), a recently developed diagnostic method, has proved to be useful in the study of patients with prostatic disease. The authors report their experience with this technique in 10 cases of advanced prostate cancer (stage D) treated with LH-RH agonists (HOE 766-Buserelin). NMR provided info...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000281218
更新日期:1986-01-01 00:00:00
abstract::The identification and diagnosis of thyroid metastases from renal adenocarcinoma are rare in living patients, though more frequent during autopsy. A case is reported in which the identification of thyroid metastases indicated a clear-cell renal carcinoma in a kidney treated 12 years earlier for emptying of multiple cy...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000281389
更新日期:1988-01-01 00:00:00
abstract::In order to determine an optimum mode for systemic administration of recombinant interleukin 2 (rIL2), the effects of rIL2 on lymphocyte-mediated cytotoxicity against renal carcinoma cells were studied in vitro. Augmentation of the cytotoxicity by rIL2 was dose- and time-dependent. The optimum dose of rIL2 was 100-500...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282272
更新日期:1991-01-01 00:00:00
abstract::In unilateral kidney disease, it has been suggested that the ipsilateral state could be estimated by measuring contralateral kidney size; thus, a contralateral increase should reflect an ipsilateral impairment and vice versa. This could easily be performed using ultrasound. In our experiments with partial obstruction ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282898
更新日期:1996-01-01 00:00:00
abstract:PURPOSE:We aimed at evaluating the efficacy of extracorporeal shockwave lithotripsy (SWL) for treating distal ureteral calculi performed in supine vs. prone position. MATERIALS AND METHODS:Eligible studies were identified by 2 reviewers using PubMed, Embase, and Web of Science databases. Outcomes included stone-free r...
journal_title:Urologia internationalis
pub_type: 杂志文章,meta分析,评审
doi:10.1159/000439140
更新日期:2016-01-01 00:00:00
abstract::Urethral diverticula are pouches opening into the urethral canal that may be either acquired or congenital. Diverticula of the female urethra are fairly common. However, diverticula of the male urethra are relatively uncommon abnormalities. Clinical manifestation is frequently described with irritative and/or obstruct...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000346321
更新日期:2013-01-01 00:00:00
abstract::Sacrocolpopexy, a surgical technique with a low morbidity rate, is a valid procedure for repairing vaginal vault prolapse. To our knowledge, only 1 case of rectum erosion after open sacrocolpopexy has been reported in the literature, and there is no record of any such incident after laparoscopic sacrocolpopexy. We rep...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000353421
更新日期:2014-01-01 00:00:00
abstract::The purpose of this study is to report the safety and efficiency of laparoscopic varicocelectomy in 100 consecutive patients operated at a single teaching institution. There were three indications: (1) infertility associated with oligo-astheno-teratospermia (n = 52); (2) chronic dragging sensation of the left testicle...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282986
更新日期:1997-01-01 00:00:00
abstract::Non-clear cell renal cell carcinoma accounts for about 20% of all kidney cancers. Tubulocystic carcinoma of the kidney (TCK) is a newly described entity with distinct molecular, genetic and clinical features. In 2013, it was one of five new renal tumors to be recognized by the International Society of Urological Patho...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000366287
更新日期:2015-01-01 00:00:00
abstract::Membrane fluidity and lipid composition of rat testis in experimental bilateral cryptorchidism were studied. Membrane fluidity measured by fluorescence anisotropy of diphenylhexatriene decreased on the 3rd day of intraabdominal fixation, at an early stage of cryptorchidism. Lipid composition of testicular membrane was...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000281725
更新日期:1990-01-01 00:00:00
abstract:INTRODUCTION:Etiology of serum anti-p53 antibodies in bladder cancer patients is still unknown. In this study we evaluated the relationship between serum anti-p53 antibodies and microvessel density in bladder cancer patients. MATERIALS AND METHODS:Seventy-six patients with transitional cell carcinoma of the urinary bl...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000080831
更新日期:2004-01-01 00:00:00
abstract:INTRODUCTION:Partial unilateral ureteral obstruction (PUUO) is the type of obstruction that is most often encountered in pediatric clinical practice. The majority of our knowledge on PUUO has been derived from experimental studies and the effects of PUUO on the kidney have still been a source of continual investigation...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000111736
更新日期:2008-01-01 00:00:00
abstract::Adrenal cysts are very rare lesions, usually asymptomatic or without characteristic symptoms. They are classified as pseudocysts, endothelial cysts, epithelial cysts or parasitic cysts. Although pseudocysts are reported to be the most common clinically recognized adrenal cysts in surgical series, endothelial cysts are...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000111726
更新日期:2008-01-01 00:00:00