Increase of virulence and its phenotypic traits in drug-resistant strains of Candida albicans.


:There is concern about the rise of antifungal drug resistance, but little is known about comparative biological properties and pathogenicity of drug-resistant strains. We generated fluconazole (FLC; CO23 RFLC)- or micafungin (FK; CO23 RFK)-resistant strains of Candida albicans by treating a FLC- and FK-susceptible strain of this fungus (CO23 S) with stepwise-increasing concentrations of either drug. Molecular analyses showed that CO23 RFLC had acquired markedly increased expression of the drug-resistance efflux pump encoded by the MDR1 gene, whereas CO23 RFK had a homozygous mutation in the FSK1 gene. These genetic modifications did not alter to any extent the growth capacity of the drug-resistant strains in vitro, either at 28 degrees C or at 37 degrees C, but markedly increased their experimental pathogenicity in a systemic mouse infection model, as assessed by the overall mortality and target organ invasion. Interestingly, no apparent increase in the vaginopathic potential of the strains was observed with an estrogen-dependent rat vaginal infection. The increased pathogenicity of drug-resistant strains for systemic infection was associated with a number of biochemical and physiological changes, including (i) marked cellular alterations associated with a different expression and content of major cell wall polysaccharides, (ii) more rapid and extensive hypha formation in both liquid and solid media, and (iii) increased adherence to plastic and a propensity for biofilm formation. Overall, our data demonstrate that experimentally induced resistance to antifungal drugs, irrespective of drug family, can substantially divert C. albicans biology, affecting in particular biological properties of potential relevance for deep-seated candidiasis.


Angiolella L,Stringaro AR,De Bernardis F,Posteraro B,Bonito M,Toccacieli L,Torosantucci A,Colone M,Sanguinetti M,Cassone A,Palamara AT




Has Abstract


2008-03-01 00:00:00














  • Treatment of anaerobic infections with metronidazole.

    abstract::The results of treatment of 10 patients with anaerobic infections with metronidazole are presented. Six patients were cured, three showed initial good response but circumstances required a change to another drug, and one patient did not respond. The unique spectrum of the drug, its pharmacology, and limitations are di...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 临床试验,杂志文章


    authors: Tally FP,Sutter VL,Finegold SM

    更新日期:1975-05-01 00:00:00

  • Protein O-mannosyltransferase isoforms regulate biofilm formation in Candida albicans.

    abstract::Five isoforms of protein mannosyltransferase (Pmt) O-mannosylate secretory proteins in Candida albicans. pmt mutants were differentially defective for biofilm formation on plastic in static and flow-through systems, and a Pmt inhibitor blocked early stages of biofilm formation. Conceptually, Pmt inhibition may prevent...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Peltroche-Llacsahuanga H,Goyard S,d'Enfert C,Prill SK,Ernst JF

    更新日期:2006-10-01 00:00:00

  • Safety and pharmacokinetics of XOMA 3AB, a novel mixture of three monoclonal antibodies against botulinum toxin A.

    abstract::Botulinum neurotoxin A is a category A bioterrorism agent. Current antitoxin therapies are scarce and produce adverse reactions. XOMA 3AB consists of 3 IgG1 monoclonal antibodies (MAbs), each with a distinct human or humanized variable region, which bind to distinct epitopes on botulinum neurotoxin serotype A. This fi...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 临床试验,杂志文章


    authors: Nayak SU,Griffiss JM,McKenzie R,Fuchs EJ,Jurao RA,An AT,Ahene A,Tomic M,Hendrix CW,Zenilman JM

    更新日期:2014-09-01 00:00:00

  • Differential expression of methicillin resistance by different biofilm-negative Staphylococcus epidermidis transposon mutant classes.

    abstract::Biofilm formation mediated by polysaccharide intercellular adhesin (PIA) is the major virulence factor of Staphylococcus epidermidis and is often associated with methicillin resistance. Transposon Tn917 insertions leading to a biofilm-negative phenotype in the biofilm-producing S. epidermidis strain 1457 (mecA-negativ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Mack D,Sabottke A,Dobinsky S,Rohde H,Horstkotte MA,Knobloch JK

    更新日期:2002-01-01 00:00:00

  • Use of real-time PCR and fluorimetry to detect lamivudine resistance-associated mutations in hepatitis B virus.

    abstract::Very rapid amplification of DNA by PCR in small volumes can be continuously monitored by the detection of the binding of probes with a rapid cycler with built-in fluorometric detection. Primers were designed to amplify approximately 100 bp of the polymerase gene of hepatitis B virus (HBV) spanning codon 550, where mut...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Cane PA,Cook P,Ratcliffe D,Mutimer D,Pillay D

    更新日期:1999-07-01 00:00:00

  • Daptomycin susceptibility of unusual gram-positive bacteria: comparison of results obtained by the Etest and the broth microdilution method.

    abstract::MICs of daptomycin, linezolid, and vancomycin against 212 isolates, including Listeria monocytogenes and Pediococcus, Leuconostoc, Rhodococcus, and Nocardia spp., were determined by the broth microdilution method; daptomycin MICs were also determined by the Etest. Except with those for Leuconostoc spp., daptomycin Ete...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Huang YT,Liao CH,Teng LJ,Hsueh PR

    更新日期:2007-04-01 00:00:00

  • Biliary excretion of moxalactam.

    abstract::The biliary excretion of moxalactam was studied in 11 postsurgery patients who had indwelling T-tubes inserted in their common bile ducts. Peak levels of moxalactam in the bile reached mean levels of 33.7 +/- 11.1 and 173.7 +/- 67.0 micrograms/ml 2 h after intravenous administration of a single 500- or 2,000-mg dose, ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Martinez OV,Levi JU,Livingstone A,Malinin TI,Zeppa R,Hutson D,Einhorn N

    更新日期:1981-08-01 00:00:00

  • Influence of Reactive Oxygen Species on De Novo Acquisition of Resistance to Bactericidal Antibiotics.

    abstract::The radical-based theory proposes that three major classes of bactericidal antibiotics, i.e., β-lactams, quinolones, and aminoglycosides, have in common the downstream formation of lethal levels of reactive oxygen species (ROS) as part of the killing mechanism. If bactericidal antibiotics exhibit a common mechanism, t...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Hoeksema M,Brul S,Ter Kuile BH

    更新日期:2018-05-25 00:00:00

  • First detection of metallo-beta-lactamase VIM-2 in Pseudomonas aeruginosa isolates from Colombia.

    abstract::Carbapenem resistance rates in Pseudomonas aeruginosa isolates in Colombia, as in many South American countries, are high for reasons that remain unclear. From our nationwide network, we describe the first detection of the metallo-beta-lactamase VIM-2 in clinical isolates of P. aeruginosa from multiple cities within C...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Villegas MV,Lolans K,del Rosario Olivera M,Suarez CJ,Correa A,Queenan AM,Quinn JP,Colombian Nosocomial Resistance Study Group.

    更新日期:2006-01-01 00:00:00

  • Triple crossover study on absorption and excretion of ampicillin, pivampicillin, and amoxycillin.

    abstract::The absorption and excretion of equivalent doses of ampicillin, pivampicillin, and amoxycillin were compared in 10 healthy volunteers after administration of these antibiotics in a randomized triple crossover fashion. As recommended, ampicillin and amoxycillin were administered on an empty stomach, whereas pivampicill...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Verbist L

    更新日期:1974-11-01 00:00:00

  • Single-dose pharmacokinetics of ceftibuten (SCH 39720) in infants and children.

    abstract::Ceftibuten (CFB), a new broad-spectrum cephalosporin for oral administration, possesses potent activity in vitro against a wide range of gram-negative and certain gram-positive pathogens frequently encountered in pediatric patients. Its antimicrobial spectrum and dosage formulation suggest a use for CFB in the treatme...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Kearns GL,Reed MD,Jacobs RF,Ardite M,Yogev RD,Blumer JL

    更新日期:1991-10-01 00:00:00

  • Fenpropimorph affects uptake of uracil and cytosine in Saccharomyces cerevisiae.

    abstract::Fenpropimorph was shown to inhibit the accumulation of the pyrimidine bases uracil and cytosine from the growth media in Saccharomyces cerevisiae. Uracil prototrophs of S. cerevisiae were more resistant to the growth-inhibitory effects of fenpropimorph than were uracil auxotrophs. High concentrations of uracil rescued...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Crowley JH,Lorenz RT,Parks LW

    更新日期:1994-05-01 00:00:00

  • Vancomycin-resistant Staphylococcus aureus isolate from a patient in Pennsylvania.

    abstract::A vancomycin-resistant Staphylococcus aureus (VRSA) isolate was obtained from a patient in Pennsylvania in September 2002. Species identification was confirmed by standard biochemical tests and analysis of 16S ribosomal DNA, gyrA, and gyrB sequences; all of the results were consistent with the S. aureus identification...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Tenover FC,Weigel LM,Appelbaum PC,McDougal LK,Chaitram J,McAllister S,Clark N,Killgore G,O'Hara CM,Jevitt L,Patel JB,Bozdogan B

    更新日期:2004-01-01 00:00:00

  • Serum protein binding of the aminoglycoside antibiotics.

    abstract::The binding of four aminoglycoside antibiotics by human serum was investigated under controlled conditions of physiological pH and temperature, by means of an ultrafiltration technique. No serum binding was demonstrable for gentamicin, tobramycin, or kanamycin, whereas streptomycin was 35% bound. Previous conflicting ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Gordon RC,Regamey C,Kirby WM

    更新日期:1972-09-01 00:00:00

  • 9-[2-(Phosphonomethoxy)propyl]adenine therapy of established simian immunodeficiency virus infection in infant rhesus macaques.

    abstract::The long-term therapeutic and toxic effects of 9-[2-(phosphonomethoxy)propyl]adenine (PMPA) were evaluated in simian immunodeficiency virus (SIV)-infected newborn rhesus macaques. Four untreated SIV-infected newborn macaques developed persistently high levels of viremia, and three of the four animals had rapidly fatal...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Van Rompay KK,Cherrington JM,Marthas ML,Berardi CJ,Mulato AS,Spinner A,Tarara RP,Canfield DR,Telm S,Bischofberger N,Pedersen NC

    更新日期:1996-11-01 00:00:00

  • Relationships among antibacterial activity, inhibition of DNA gyrase, and intracellular accumulation of 11 fluoroquinolones.

    abstract::A series of 11 fluoroquinolone antibacterial agents, including 8 newly synthesized molecules and 3 reference compounds (pefloxacin, ciprofloxacin, and sparfloxacin), were tested for their MICs against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The intracellular accumulation of fluoroquinolone...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Bazile S,Moreau N,Bouzard D,Essiz M

    更新日期:1992-12-01 00:00:00

  • Absence of a High Level of Duplication of the Plasmepsin II Gene in Africa.

    abstract::Resistance to piperaquine has been associated with the amplification of the plasmepsin II gene in Cambodia. None of the 175 African isolates that we analyzed had plasmepsin II gene amplification (piperaquine 50% inhibitory concentration ranged from 0.94 to 137.5 nM), suggesting there is a low prevalence of piperaquine...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Robert MG,Foguim Tsombeng F,Gendrot M,Mosnier J,Amalvict R,Benoit N,Torrentino-Madamet M,Pradines B,French National Reference Centre for Imported Malaria Study Group.

    更新日期:2018-10-24 00:00:00

  • Novel dengue virus NS2B/NS3 protease inhibitors.

    abstract::Dengue fever is a severe, widespread, and neglected disease with more than 2 million diagnosed infections per year. The dengue virus NS2B/NS3 protease (PR) represents a prime target for rational drug design. At the moment, there are no clinical PR inhibitors (PIs) available. We have identified diaryl (thio)ethers as c...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Wu H,Bock S,Snitko M,Berger T,Weidner T,Holloway S,Kanitz M,Diederich WE,Steuber H,Walter C,Hofmann D,Weißbrich B,Spannaus R,Acosta EG,Bartenschlager R,Engels B,Schirmeister T,Bodem J

    更新日期:2015-02-01 00:00:00

  • In vitro activity of ceftriaxone alone and in combination with gentamicin, tobramycin, and amikacin against Pseudomonas aeruginosa.

    abstract::The in vitro activity of ceftriaxone alone and in combination with gentamicin, tobramycin, and amikacin against 50 Pseudomonas aeruginosa strains was studied by the broth dilution method and the time-kill curve method. The majority of the P. aeruginosa strains tested were resistant to ceftriaxone. Combining ceftriaxon...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Watanakunakorn C

    更新日期:1983-08-01 00:00:00

  • In vitro adsorption of gentamicin and netilmicin by polyacrylonitrile and polyamide hemofiltration filters.

    abstract::Adsorption of gentamicin and netilmicin by new polyacrylonitrile and polyamide hemofiltration filters was studied over 4 h, using a single-compartment in vitro continuous venovenous hemofiltration model. After the first dose (16.6 mg of gentamicin, 19.3 mg of netilmicin), 14.9 mg of gentamicin and 19.2 mg of netilmici...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: K N Lam P,Tian Q,Ip M,Gomersall CD

    更新日期:2010-02-01 00:00:00

  • New insertion sequence elements in the upstream region of cfiA in imipenem-resistant Bacteroides fragilis strains.

    abstract::The 747-bp cfiA gene, which encodes a metallo-beta-lactamase, and the regions flanking cfiA in six imipenem-resistant and four imipenem-susceptible Bacteroides fragilis strains isolated in Japan were analyzed by PCR and DNA sequencing. The nucleotide sequences of the cfiA genes (designated cfiA(1) to cfiA(10)) of all ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Kato N,Yamazoe K,Han CG,Ohtsubo E

    更新日期:2003-03-01 00:00:00

  • In vitro efficacies and resistance profiles of rifampin-based combination regimens for biofilm-embedded methicillin-resistant Staphylococcus aureus.

    abstract::To compare the in vitro antibacterial efficacies and resistance profiles of rifampin-based combinations against methicillin-resistant Staphylococcus aureus (MRSA) in a biofilm model, the antibacterial activities of vancomycin, teicoplanin, daptomycin, minocycline, linezolid, fusidic acid, fosfomycin, and tigecycline a...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Tang HJ,Chen CC,Cheng KC,Wu KY,Lin YC,Zhang CC,Weng TC,Yu WL,Chiu YH,Toh HS,Chiang SR,Su BA,Ko WC,Chuang YC

    更新日期:2013-11-01 00:00:00

  • Detection of molecular markers of antiviral resistance in influenza A (H5N1) viruses using a pyrosequencing method.

    abstract::Resistance of influenza viruses to antiviral drugs can emerge following medication or may result from natural variation. Two classes of anti-influenza virus drugs targeting either the M2 protein (amantadine and rimantadine) or neuraminidase (NA; oseltamivir and zanamivir) are currently licensed. These drugs are expect...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Deyde VM,Nguyen T,Bright RA,Balish A,Shu B,Lindstrom S,Klimov AI,Gubareva LV

    更新日期:2009-03-01 00:00:00

  • Cellular uptake and biological effects of antisense oligodeoxynucleotide analogs targeted to herpes simplex virus.

    abstract::In this study, we synthesized antisense oligodeoxynucleotides (ODNs) with phosphodiester, phosphorothioate (S-ODNs), or methylphosphonate linkages complementary to the splicing acceptor site of immediate-early pre-mRNA 5 of herpes simplex virus type 1 (HSV-1). The antiviral activity of each analog on cytopathic effect...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Shoji Y,Shimada J,Mizushima Y,Iwasawa A,Nakamura Y,Inouye K,Azuma T,Sakurai M,Nishimura T

    更新日期:1996-07-01 00:00:00

  • Phase 1 safety and pharmacokinetic study of chimeric murine-human monoclonal antibody c alpha Stx2 administered intravenously to healthy adult volunteers.

    abstract::Hemolytic-uremic syndrome (HUS) is a serious complication of infection by Shiga toxin-producing Escherichia coli. Shiga toxin type 2 (Stx2) is responsible for the renal toxicity that can follow intestinal infection and hemorrhagic colitis due to E. coli. A chimeric mouse-human antibody, designated c alpha Stx2, that h...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 临床试验,杂志文章


    authors: Dowling TC,Chavaillaz PA,Young DG,Melton-Celsa A,O'Brien A,Thuning-Roberson C,Edelman R,Tacket CO

    更新日期:2005-05-01 00:00:00

  • Chlorhexidine resistance in methicillin-resistant Staphylococcus aureus or just an elevated MIC? An in vitro and in vivo assessment.

    abstract::Chlorhexidine (Hibiscrub; ICI) is generally accepted to be effective as an antiseptic hand wash for methicillin-susceptible Staphylococcus aureus (MSSA), but there is dispute whether the chlorhexidine MIC for methicillin-resistant S. aureus (MRSA) strains is higher than that for MSSA strains and, indeed, whether it is...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Cookson BD,Bolton MC,Platt JH

    更新日期:1991-10-01 00:00:00

  • Efficacy of trovafloxacin, a new quinolone antibiotic, in experimental staphylococcal endocarditis due to oxacillin-resistant strains.

    abstract::Therapeutic options for severe infections caused by strains of oxacillin-resistant Staphylococcus aureus (ORSA) and coagulase-negative staphylococci (ORSE) are very limited. With the increasing resistance of such strains to aminoglycosides, rifampin, and currently available quinolone agents, as well as the recent docu...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Bayer AS,Li C,Ing M

    更新日期:1998-07-01 00:00:00

  • Inhibition of Pneumocystis carinii dihydropteroate synthetase by para-acetamidobenzoic acid: possible mechanism of action of isoprinosine in human immunodeficiency virus infection.

    abstract::Isoprinosine has been reported to decrease progression to AIDS, primarily by preventing Pneumocystis carinii pneumonia (PCP), in human immunodeficiency virus-infected patients, but the mechanism of action is unknown. para-Acetamidobenzoic acid (PAcBA), one component of isoprinosine, is structurally related to para-ami...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Kovacs JA,Powell F,Voeller D,Allegra CJ

    更新日期:1993-06-01 00:00:00

  • Reconstruction of an active integron recombination site after integration of a gene cassette at a secondary site.

    abstract::As the site of insertion of the aadB gene cassette on pRAY, from a clinical isolate of Acinetobacter, is almost identical to the preferred site on integrons, the composite 59-base element (59-BE) associated with this cassette is potentially recombinationally active. By using a conduction assay to quantitate site activ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Segal H,Francia MV,Lobo JM,Elisha G

    更新日期:1999-10-01 00:00:00

  • Comparative in vitro activity of cefbuperazone against anaerobic bacteria isolated from community hospitals.

    abstract::The activity of cefbuperazone against 266 strains of anaerobic bacteria was determined by the agar dilution method and compared with cefoxitin, moxalactam, piperacillin, and clindamycin. All strains were recent clinical isolates from community hospitals. All agents tested showed good activity against Bacteroides fragi...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Goldstein EJ,Citron DM

    更新日期:1985-02-01 00:00:00