Effect of prolonged vaginal distention and sphincter transection on physiologic function of the external anal sphincter in an animal model.

Abstract:

OBJECTIVE:To estimate the effect of prolonged vaginal distention and anal sphincter transection on contractile properties of the external anal sphincter. METHODS:Young female virgin rats were randomly assigned to four treatment groups (sham, vaginal distention, transection of anal sphincter plus repair, or combined distention and transection plus repair). After 3 weeks, the anal sphincter complex was analyzed for twitch tension, maximal tetanic force, fatigue, and maximal responses to electrical field stimulation. Each sphincter was serially cross-sectioned and stained with hematoxylin and eosin to evaluate sphincter integrity. Statistical evaluation was performed using analysis of variance (Student-Newman-Keuls) and chi2 analysis. RESULTS:The function of a transected and repaired anal sphincter is compromised significantly after 3 weeks. Vaginal distention alone did not alter function of the sphincter. Further, prolonged vaginal distention together with sphincter transection did not result in statistically significant differences in sphincter function compared with transection alone. CONCLUSION:In an animal model, there is a major effect on external anal sphincter function in vitro 3 weeks after laceration with repair. Although prolonged vaginal distention had no significant effect alone and minimal adverse effects on the lacerated anal sphincter, laceration of the sphincter has major adverse effects on its morphology and function. LEVEL OF EVIDENCE:II.

journal_name

Obstet Gynecol

authors

Wai CY,Miller RT,Word RA

doi

10.1097/AOG.0b013e318162f6a7

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

332-40

issue

2 Pt 1

eissn

0029-7844

issn

1873-233X

pii

111/2/332

journal_volume

111

pub_type

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