Abstract:
:In the dairy cow, puerperal uterine intra-luminal concentrations of PGE(2) are related to the establishment and severity of uterine infections. Here we evaluated whether the blood concentrations of PGE(2) and the gene transcription profiles of enzymes involved in its synthesis (cyclooxygenase-2 and prostaglandin E synthase) could be used as markers of predisposition and/or presence of puerperal uterine infections. We also studied the relationship between the endocrine status and the leukocyte profiles around parturition and the transcription patterns of the genes. Finally, we have characterized the in vitro gene transcription and expression response to a challenge of LPS. Gene transcription profiles, quantified by real-time PCR, were similar in normal puerperium and metritis/endometritis cows, indicating that they are not suitable markers of predisposition to/presence of puerperal uterine infections. Transcription decreased from 2 weeks before parturition until parturition, when a minimum was attained, and then increased during the first week postpartum. The lowest gene transcription, at parturition, was coincidental with the highest total leukocytes, polymorphonuclear neutrophils and CD14 positive cell numbers. It is suggested that by this mechanism, a large number of PMN can be recruited into the uterus after parturition, avoiding an excessive acute inflammatory response. The lowest gene transcription was also coincidental with the surge in cortisol concentrations, indicating that this hormone plays a main immunomodulatory role around parturition. Gene transcription was significantly greater after stimulation with LPS than in non-stimulated blood. We suggest that this PGE(2) producing cells might arrive to the uterine lumen, contributing to the local PGE(2) concentrations and mediating the inflammatory response.
journal_name
Domest Anim Endocrinoljournal_title
Domestic animal endocrinologyauthors
Silva E,Gaivão M,Leitão S,Amaro A,Costa LL,Mateus Ldoi
10.1016/j.domaniend.2008.07.001subject
Has Abstractpub_date
2008-10-01 00:00:00pages
314-23issue
3eissn
0739-7240issn
1879-0054pii
S0739-7240(08)00076-3journal_volume
35pub_type
杂志文章abstract::The objective of this study was to characterize plasma insulin-like growth factor-I (IGF-I) profiles during the estrous cycle in goats. Frequent blood samples were drawn during the day of estrus and during the luteal phase on Day 10 after estrus, and plasma growth hormone (GH) and IGF-I profiles were examined. Then, d...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/s0739-7240(99)00082-x
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abstract::Three experiments were performed to study effects of decreased concentrations of estradiol-17 beta (E2) on lifespan and function of ensuing ovine corpora lutea (CL). In experiment 1, 52 follicles were collected from 10 ewes and placed into individual culture with 0 or .01 microCi 3H-androstenedione (10 ng; 3H-A) and 0...
journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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更新日期:2014-01-01 00:00:00
abstract::It has now been well documented that the normal and tumorous canine mammary glands can be extra-pituitary sites of substantial growth hormone (GH) synthesis. Until now, attempts to reproduce the GH synthesis in-vitro using canine mammary explants or mammary tumor cells have not been successful. Therefore, the response...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/s0739-7240(01)00088-1
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journal_title:Domestic animal endocrinology
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更新日期:2002-06-01 00:00:00
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journal_title:Domestic animal endocrinology
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更新日期:1999-01-01 00:00:00
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2013.01.005
更新日期:2013-05-01 00:00:00
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journal_title:Domestic animal endocrinology
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更新日期:1988-04-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2013.11.006
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2004.01.008
更新日期:2004-08-01 00:00:00
abstract::The aim of this work was to study the role of hepatic metabolism of compensatory growth in piglets induced by protein restriction and subsequent protein realimentation. Thirty-six weaned piglets were randomly distributed in a control group and a treatment group. The control group piglets were fed with a normal protein...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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更新日期:2021-01-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2020.106538
更新日期:2021-01-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2005.03.003
更新日期:2005-11-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2014.12.001
更新日期:2015-04-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2009.08.004
更新日期:2010-02-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.domaniend.2016.02.014
更新日期:2016-07-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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更新日期:1998-11-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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更新日期:1999-04-01 00:00:00
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
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更新日期:2002-07-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
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更新日期:2000-08-01 00:00:00
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journal_title:Domestic animal endocrinology
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更新日期:1997-09-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2010.03.002
更新日期:2010-08-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.domaniend.2005.02.005
更新日期:2005-08-01 00:00:00
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
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更新日期:1988-01-01 00:00:00
abstract::To determine if poor maternal nutrition alters growth, body composition, circulating growth factors, and expression of genes involved in the development of muscle and adipose of offspring, 24 Dorset and Shropshire ewes were fed either 100% (control fed), 60% (restricted fed), or 126% (over fed) of National Research Co...
journal_title:Domestic animal endocrinology
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