Abstract:
:The effects of 15-hydroperoxy metabolites of arachidonic acid on vascular tone were evaluated in the perfused mesenteric preparation, the isolated perfused lung and segments of pulmonary arteries of the rat. In the mesenteric preparation, precontracted with phenylephrine, both 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid (15-HPETE, ED50 1.6 nmol) and 8,15-dihydroperoxy-5,9,11,13-eicosatetraenoic acid (8,15-diHPETE, ED50 0.3 nmol) induced dose-dependent vasodilatation, whereas 5,15-diHPETE (0.2-100 nmol) had no effect. Prostacyclin (ED50 0.01 nmol) was, however, more potent than the hydroperoxides. In the rat isolated lung, precontracted with the stable thromboxane agonist U-46619, dose-dependent decrease in the perfusion pressure occurred with 15-HPETE(ED50 40 nmol), 5,15-diHPETE (ED50 30 nmol) and 8, 15-diHPETE (ED50 7 nmol) while 13-hydroperoxide of linoleic acid had no effect. Prostacyclin was 10 times more potent than 8, 15-diHPETE. The vasodilator effects were not affected by indomethacin. In both endothelium intact and denuded rat pulmonary arteries the hydroperoxides 15-HPETE, 8,15-diHPETE, and 5,15-diPETE induced dose-dependent relaxation. The hydroperoxide, 8,15-diHPETE was at least 3 times more potent than 15-HPETE or 5,15--diHPETE. The hydroperoxides had no effect on the basal tone of vessel segments and the relaxation induced by 15-HPETE was not attenuated by methylene blue (5 microM). These data indicate that 8,15-diHPETE may be a significant endothelium-independent vasodilator product of arachidonate lipoxygenation.
journal_name
Br J Pharmacoljournal_title
British journal of pharmacologyauthors
d'Alarcao M,Corey EJ,Cunard C,Ramwell P,Uotila P,Vargas R,Wroblewska Bdoi
10.1111/j.1476-5381.1987.tb11256.xsubject
Has Abstractpub_date
1987-07-01 00:00:00pages
627-32issue
3eissn
0007-1188issn
1476-5381journal_volume
91pub_type
杂志文章abstract::1. In the present work, we have studied the expression of Fos during acute and chronic administration of the kappa-opioid receptor agonist U-50488H and after U-5088H withdrawal in the rat hypothalamic paraventricular nucleus (PVN). Fos production was also studied in brainstem regions that innervate the PVN: the A(2) c...
journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1038/sj.bjp.0705179
更新日期:2003-04-01 00:00:00
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journal_title:British journal of pharmacology
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journal_title:British journal of pharmacology
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doi:10.1111/j.1476-5381.1980.tb07937.x
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journal_title:British journal of pharmacology
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更新日期:1990-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1476-5381.1990.tb14075.x
更新日期:1990-08-01 00:00:00
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journal_title:British journal of pharmacology
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更新日期:2017-01-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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abstract::1. Muscarinic receptors present in the human astrocytoma cell line 1321 N1 were characterized in radioligand binding studies and in functional studies of carbachol-stimulated phosphatidylinositol (PI) turnover. 2. In radioligand binding studies the muscarinic receptor in intact cells could be labelled using [3H]-N-met...
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更新日期:1989-02-01 00:00:00
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journal_title:British journal of pharmacology
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更新日期:2007-07-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00
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更新日期:1998-04-01 00:00:00
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更新日期:2007-02-01 00:00:00
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更新日期:1999-07-01 00:00:00
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journal_title:British journal of pharmacology
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更新日期:1996-03-01 00:00:00
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更新日期:1986-07-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:1995-03-01 00:00:00
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更新日期:1996-02-01 00:00:00
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更新日期:1973-10-01 00:00:00
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journal_title:British journal of pharmacology
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更新日期:2003-10-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:2020-09-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章,评审
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更新日期:2019-06-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1991.tb09809.x
更新日期:1991-06-01 00:00:00
abstract::1. The action of adrenaline on the K(+) balance of the isolated heart was found to depend on the ionic composition and the temperature of the perfusion fluids used.2. When the perfusion fluid contained 145 mM Na(+), 1.7 mM Ca(++) and 3-9 mM K(+), adrenaline caused the hearts to gain K(+); when the K(+) concentration w...
journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1969.tb08012.x
更新日期:1969-07-01 00:00:00