Abstract：
:An automated ligand-receptor binding study was performed for the first time using solid-phase microextraction (SPME) coupled to liquid chromatography-tandem mass spectrometry. A new multi-fibre SPME system, which relies on multi-well plate technology and allows parallel preparation of up to 96 samples was used in order to obtain all data points of the binding curve in a single experiment. The binding of diazepam to human serum albumin was used as the model system in order to evaluate the performance of automated SPME. The time required to establish equilibrium was 30 min; this was verified experimentally by constructing extraction time profiles in the presence and absence of receptor molecules. Fibre constant calibration was used to remove inter-fibre variability from the binding data. Using a simple one-site binding model, a binding constant of 9.1 x 10(5)+/-3 x 10(5)l/mol was obtained. This result is in excellent agreement with values for equilibrium dialysis and manual SPME procedures reported in the literature. The proposed method can be further extended to study plasma-protein binding or drug binding to whole blood. In comparison to other methods, SPME is simpler, faster and fully automated, can be combined with any analytical detection method, and can be used to directly study complex samples.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Vuckovic D,Pawliszyn Jdoi
10.1016/j.jpba.2008.08.023subject
Has Abstractpub_date
2009-11-01 00:00:00pages
550-5issue
4eissn
0731-7085issn
1873-264Xpii
S0731-7085(08)00449-4journal_volume
50pub_type
杂志文章abstract：:Ginseng saponins isolated from ginseng, have been regarded as the principal constituents responsible for the biological activities. The aim of this study was to set up a liquid chromatography-mass spectrometry (LC-MS) method for simultaneously determine the concentration of Ginsenoside Rg1 and its secondary glycoside ...
journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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pub_type： 杂志文章,评审
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章,评审
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
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