Abstract：
PURPOSE:Trastuzumab (Herceptin™) is often internalized following binding to Her2+ tumor cells. The objective of this study was to investigate whether trastuzumab can be used as a specific carrier to deliver antisense oligomers into Her2+ tumor cells both in vitro and in vivo. METHODS:A biotinylated MORF oligomer antisense to RhoC mRNA and its biotinylated sense control were labeled with either lissamine for fluorescence detection or 99mTc for radioactivity detection and were linked to biotinylated trastuzumab via streptavidin. The nanoparticles were studied in SUM190 (RhoC+, Her2+) study and SUM149 (RhoC+, Her2−) control cells in culture and as xenografts in mice. RESULTS:As evidence of unimpaired Her2+ binding of trastuzumab within the nanoparticle, accumulations were clearly higher in SUM190 compared to SUM149 cells and, by whole-body imaging, targeting of SUM190 tumor was similar to that expected for a radiolabeled trastuzumab. As evidence of internalization, fluorescence microscopy images of cells grown in culture and obtained from xenografts showed uniform cytoplasm distribution of the lissamine-MORF. An invasion assay showed decreased RhoC expression in SUM190 cells when incubated with the antisense MORF nanoparticles at only 100 nM. CONCLUSION:Both in cell culture and in animals, the nanoparticle with trastuzumab as specific carrier greatly improved tumor delivery of the antisense oligomer against RhoC mRNA into tumor cells overexpressing Her2 and may be of general utility.
journal_name
Eur J Nucl Med Mol Imagingauthors
Wang Y,Liu X,Chen L,Cheng D,Rusckowski M,Hnatowich DJdoi
10.1007/s00259-009-1201-2subject
Has Abstractpub_date
2009-12-01 00:00:00pages
1977-86issue
12eissn
1619-7070issn
1619-7089journal_volume
36pub_type
杂志文章abstract：PURPOSE:The molecular features isocitrate dehydrogenase (IDH) mutation and 1p/19q co-deletion have gained major importance for both glioma typing and prognosis and have, therefore, been integrated in the World Health Organization (WHO) classification in 2016. The aim of this study was to characterize static and dynamic...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-017-3846-6
更新日期：2018-03-01 00:00:00
abstract：PURPOSE:To evaluate the sensitivity and specificity of PET/CT findings in PMR and generate a diagnostic algorithm utilizing a minimum number of musculoskeletal sites. METHODS:Steroid-naïve patients with newly diagnosed PMR (2012 EULAR/ACR classification criteria) were prospectively recruited to undergo whole-body 18F-...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-020-04731-z
更新日期：2020-09-01 00:00:00
abstract：:This study investigated the feasibility of ordered subsets expectation maximisation (OS-EM) reconstruction of 180 degrees pinhole single-photon emission tomography (SPET) acquired in body contour mode (variable distance between the detector and the axis of rotation for each projection) with or without a tilted detecto...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-003-1213-2
更新日期：2003-09-01 00:00:00
abstract：PURPOSE:The high failure rates in the radiotherapy (RT) target volume suggest that patients with locally advanced oesophageal cancer (LAOC) would benefit from increased total RT doses. High 2-deoxy-2-[(18)F]fluoro-D-glucose (FDG) uptake (hotspot) on pre-RT FDG positron emission tomography (PET)/CT has been reported to ...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-015-3004-y
更新日期：2015-05-01 00:00:00
abstract：PURPOSE:According to the updated WHO classification of gliomas with its emphasis on molecular parameters, tumours with an IDH-wildtype status have a dismal prognosis. To ensure timely adjustment of treatment, demand for non-invasive prediction methods is high. 18F-FET PET has been shown to be an important diagnostic to...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-019-04477-3
更新日期：2019-11-01 00:00:00
abstract：PURPOSE:(18)F-8-cyclopentyl-3-(3-fluoropropyl)-1-propylxanthine ((18)F-CPFPX) is a potent radioligand to study human cerebral A(1) adenosine receptors and their neuromodulatory and neuroprotective functions with positron emission tomography (PET). The purpose of this study was to determine the biodistribution and the r...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-008-0753-x
更新日期：2008-08-01 00:00:00
abstract：INTRODUCTION:Tumor hypoxia is a centerpiece of disease progression mechanisms such as neoangiogenesis or aggressive hypoxia-resistant malignant cells selection that impacts on radiotherapy strategies. Early identification of regions at risk for recurrence and prognostic-based classification of patients is a necessity t...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
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更新日期：2020-06-01 00:00:00
abstract：PURPOSE:We aimed to compare the standardized central review of (18)F-fluorodeoxyglucose (FDG) positron emission tomography (PET)/CT scans performed after induction therapy for follicular lymphoma (FL) in the PRIMA study (Salles et al., Lancet 377:42-51, 2011; Trotman et al., J Clin Oncol 29:3194-3200, 2011) to scan rev...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-013-2441-8
更新日期：2014-03-01 00:00:00
abstract：PURPOSE:The aim of this study was to investigate the influence of a computer-based decision support system (DSS) on performance and inter-observer variability of interpretations regarding ischaemia and infarction in myocardial perfusion scintigraphy (MPS). METHODS:Seven physicians independently interpreted 97 MPS stud...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-008-0807-0
更新日期：2008-09-01 00:00:00
abstract：PURPOSE:This meta-analysis summarized the accuracy of stress myocardial perfusion scintigraphy (MPS) and stress echocardiography for the diagnosis of coronary artery disease (CAD) in patients with arterial hypertension. METHODS:We searched for studies in which stress MPS or stress echocardiography were performed to de...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,meta分析
doi：10.1007/s00259-011-1891-0
更新日期：2011-11-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-019-04676-y
更新日期：2020-08-01 00:00:00
abstract：PURPOSE:We evaluated the value of reduced global and segmental absolute stress myocardial blood flow (sMBF) quantified by [15O] water positron emission tomography (PET) for predicting cardiac events in patients with suspected obstructive coronary artery disease (CAD). METHODS:Global and segmental sMBF during adenosine...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-020-05093-2
更新日期：2020-11-11 00:00:00
abstract：PURPOSE:Percutaneous transluminal coronary angioplasty (PTCA) is one of the main therapy options for patients with coronary artery disease (CAD), resulting in an improvement in myocardial perfusion and exercise capacity. Nevertheless, studies have also demonstrated a positive effect of regular exercise training on myoc...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-005-1768-1
更新日期：2005-07-01 00:00:00
abstract：PURPOSE:Cervical dystonia (CD) is associated with tremor/jerks (50%) and psychiatric complaints (17-70%). The dopaminergic system has been implicated in the pathophysiology of CD in animal and imaging studies. Dopamine may be related to the motor as well as non-motor symptoms of CD. CD is associated with reduced striat...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-017-3664-x
更新日期：2017-08-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-006-0155-x
更新日期：2006-12-01 00:00:00
abstract：PURPOSE:The nucleoside analogue 3'-deoxy-3'-(18)F-fluorothymidine (FLT) has recently been introduced for imaging cell proliferation with positron emission tomography (PET). We prospectively evaluated whether FLT uptake reflects proliferative activity as indicated by the Ki-67 index in non-small cell lung cancer (NSCLC)...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-007-0449-7
更新日期：2007-10-01 00:00:00
abstract：INTRODUCTION:Mastocytosis is a clonal haematological disease characterized by uncontrolled proliferation and the activation of mast cells. The value of FDG-PET/CT (FDG-PET) in mastocytosis has yet to be determined. METHODS:We retrospectively identified patients with an established diagnosis of systemic mastocytosis (S...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,多中心研究
doi：10.1007/s00259-015-3117-3
更新日期：2015-12-01 00:00:00
abstract：PURPOSE:To evaluate the diagnostic potential of PET/MRI with [(18)F]FDG in recurrent ovarian and cervical cancer in comparison to PET/CT. METHODS:A group of 19 patients with suspected recurrence of pelvic malignancies (ovarian cancer, 11 patients; cervical cancer, 8 patients) scheduled for an [(18)F]FDG PET/CT were su...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
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更新日期：2015-01-01 00:00:00
abstract：PURPOSE:Our purpose was to examine the prognostic value of post-CRT PET based on the presence or absence of FDG-avid metastatic lymph node(s) and metabolic response of the primary tumor in patients with clinically node-positive ESCC treated with definitive chemoradiotherapy (dCRT). METHODS:We identified 108 eligible p...
journal_title：European journal of nuclear medicine and molecular imaging
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更新日期：2018-05-01 00:00:00
abstract：PURPOSE:The extent of neovascularization determines the clinical outcome of coronary artery disease and other occlusive cardiovascular disorders. Monitoring of neovascularization is therefore highly important. This review article will elaborately discuss preclinical studies aimed at validating new nuclear angiogenesis ...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-016-3480-8
更新日期：2016-12-01 00:00:00
abstract：PURPOSE:Extracapsular spread (ECS) to the cervical lymph nodes is a major adverse prognostic factor in oral cavity squamous cell carcinoma (OSCC). We prospectively examined the value of FDG PET immediately before postoperative radiotherapy/concurrent chemoradiotherapy (pre-RT/CCRT PET) to detect residual/relapsing dise...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
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更新日期：2012-06-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
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更新日期：2017-02-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
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更新日期：2016-08-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,实务指引
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更新日期：2008-04-01 00:00:00
abstract：PURPOSE:To elucidate the prognostic role of post-therapeutic (18)F-fluorodeoxyglucose ((18)F-FDG) positron emission tomography (PET), we conducted a retrospective cohort study analysing the clinical factors that affect overall survival after non-operative therapy for unresectable hepatocellular carcinoma (HCC). METHOD...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
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更新日期：2010-03-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
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更新日期：2003-07-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
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更新日期：2007-08-01 00:00:00
abstract：:Assessing joint disorders has been a relatively recent and evolving application of 18F-2-fluoro-2-deoxy-D-glucose (FDG) positron emission tomography (PET) imaging. FDG is taken up by inflammatory cells, particularly when they are active as part of an ongoing inflammatory process. Hence FDG PET has been employed to ass...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-011-1863-4
更新日期：2011-10-01 00:00:00
abstract：PURPOSE:In vivo Alzheimer's disease (AD) biomarkers for tau pathology are cerebrospinal fluid (CSF) phosphorylated tau (p-tau) and [18F]flortaucipir positron emission tomography (PET). Our aim was to assess associations between CSF p-tau with [18F]flortaucipir PET and the associations of both tau biomarkers with cognit...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
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更新日期：2020-11-01 00:00:00
abstract：PURPOSE:Despite recent advances in lumbar spine stabilization surgery (LSSS), a high number of patients continue to complain of persistent/recurrent lumbar pain after LSSS. Conventional imaging (plain radiography, CT and MRI) is commonly performed to assess potential lumbar pain generators, but findings are equivocal i...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-018-4141-x
更新日期：2019-04-01 00:00:00
