Abstract：
:Stimulation of complex chaperone activity may be a viable means of therapy for neurodegenerative diseases. These chaperons execute reactivation of thermally and chemically aggregated protein substrates by cooperating with their partner co-chaperons. We optimized the expression and purification conditions of Tid1-L chaperone. Expression of Tid1-L in E. coli resulted in the formation of inclusion bodies which was further purified to soluble active form using 8 M urea and Ni-NTA column. Also, we investigated the events of the reactivation and disaggregation using aggregated G6PDH, luciferase and insulin as substrates. Incubation of aggregated/denatured enzymes with mortalin but not with Tid1 and/or Mge1 resulted in the initiation of the disaggregation reaction albeit very insignificantly. Under the same conditions coincubating the samples with chaperon and its assisted partners Tid1-L and nucleotide exchange factor Mge1 led to (40%) increase in enzyme activity of G6PDH. Similarly, luciferase activity was synergistically enhanced in the presence of mortlain/Tid1-L/Mge1 chaperones machinery. Chaperone-dependent disaggregation of thermally aggregated insulin showed that addition of Hsp70 and Hsp40 chaperones resulted in fast-track of renaissance reaction and inhibition of amyloid. The present study results conclude the quality of cell-control involves interaction of chaperon Hsp70 and its co-chaperones leading to complex formation with chemically/thermally aggregated substrate eventually causing its reactivation and disaggregation.
journal_name
Saudi J Biol Scijournal_title
Saudi journal of biological sciencesauthors
Khan MR,Khan MS,Ahmed A,Malik A,Qamar Wdoi
10.1016/j.sjbs.2020.09.006subject
Has Abstractpub_date
2020-11-01 00:00:00pages
3099-3105issue
11eissn
1319-562Xissn
2213-7106pii
S1319-562X(20)30412-5journal_volume
27pub_type
杂志文章abstract：:Teucrium (Lamiaceae) is a large and polymorphic genus distributed mainly in Europe, North Africa and in the temperate parts of Asia. In this study, the anatomical features of the leaf and stem of Teucrium polium are investigated. Teucrium has 19 taxa in Iran, which mainly grow in the Irano-Turanian region between 700 ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2014.11.002
更新日期：2015-03-01 00:00:00
abstract：:The purpose of this study was to explore the internal mechanism of lung cancer under the action of caspase recruitment domain-containing protein 9 (CARD9) and immunosuppressive cells myeloid-derived suppressor cells (MDSCs) in the Lewis lung cancer mice model. In this research, mice were selected as research objects, ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2019.09.024
更新日期：2019-12-01 00:00:00
abstract：:Presently, photo-mediated optimized synthesis of SNPs (CS-AgNPs) was carried out with the help of aqueous extracts of coconut (Cocos nucifera) outer shell fibre. Green synthesis of CS-AgNPs was undertaken under laboratory light conditions and characterized by several standard techniques such as UV-visible spectrophoto...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.11.022
更新日期：2021-01-01 00:00:00
abstract：Background & objectives:Polycystic ovary syndrome (PCOS) is the most common endocrinal disorder, and the greatest cause of infertility in women. Despite availability of individual data on impact of multiple endocrinal, reproductive and even metabolic factors in PCOS individuals, the data on the co-existence of BED and ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.08.039
更新日期：2020-12-01 00:00:00
abstract：:This study aimed to determine the phytochemical components, microbial inhibitory effectiveness and antioxidant properties of Aerva lanata plant extracts. The whole plant showed various medicinal applications in folklore and traditional medicine in various parts of the world. The organic extracts such as ethanol, ethyl...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2019.02.010
更新日期：2019-09-01 00:00:00
abstract：:Tuberculosis (TB) is a disease that affects one-third of the world's population. Although currently available TB drugs have many side effects, such as nausea, headache and gastrointestinal discomfort, no new anti-TB drugs have been produced in the past 30 years. Therefore, the discovery of a new anti-TB agent with min...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章,评审
doi：10.1016/j.sjbs.2020.07.007
更新日期：2020-09-01 00:00:00
abstract：:The formation process of algae is described inaccurately and water blooms are predicted with a low precision by current methods. In this paper, chemical mechanism of algae growth is analyzed, and a correlation analysis of chlorophyll-a and algal density is conducted by chemical measurement. Taking into account the inf...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.01.026
更新日期：2017-03-01 00:00:00
abstract：:The present study aimed to investigate the contents of glucosinolates (GSLs) and carotenoids in eleven varieties of Chinese cabbage in relation to the expression level of the important transcription factors. MS and HPLC analysis identified the presence of 13 GSLs (progoitrin, sinigrin, glucoalyssin, gluconapoleiferin,...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2016.04.004
更新日期：2018-01-01 00:00:00
abstract：:Corona viruses (CoV) are known to cause extreme pandemics in the globe. The year 2020 will be a pandemic with the spread of the novel coronavirus (SARS-CoV-2) across the globe. Coronavirus 2019 (COVID-19) has been a part of our scary life for more than a quarter of a year in 2020. The Wuhan market and China have been ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章,评审
doi：10.1016/j.sjbs.2020.11.035
更新日期：2021-01-01 00:00:00
abstract：:Shiga toxin-producing Escherichia coli (STEC) strains are considered as one of the major food-borne disease agents in humans worldwide. STEC strains, also called verotoxin-producing E. coli strains. The objectives of the present study were serotyping and molecular characterization of shiga toxigenic E. coli associated...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2015.02.019
更新日期：2015-07-01 00:00:00
abstract：Background:The antimicrobial resistance of extraintestinal pathogenic Escherichia coli (ExPEC) has progressively been reported worldwide. This resistance has been ascribed to global dissemination of a single E. coli clone, namely E. coli sequence type 131 (E. coli ST131). The main goal of this study is to determine the...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2019.09.020
更新日期：2020-01-01 00:00:00
abstract：:This study was carried out to evaluate the anti-obesity effect of Vernonia amygdalina Del. (VA) supplemented diet. VA leaf powder was fed at 5% and 15% to diet-induced obese rats for 4 weeks and its effect compared with orlistat (5.14 mg/kg p.o.), an anti-obesity drug. Food intake, body and organ weights, total body f...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2012.05.003
更新日期：2012-07-01 00:00:00
abstract：Objectives:Comparison of Ticagrelor vs clopidogrel in antiplatelet therapeutic effect of acute myocardial infarction patients undergoing percutaneous coronary intervention. Methods:The study focused on 2000 acute myocardial infarction patients undergoing percutaneous coronary intervention (PCI) in our hospital from Ja...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.11.020
更新日期：2017-12-01 00:00:00
abstract：:The BRAF gene encodes for a serine/threonine protein kinase that participates in the MAPK/ERK signalling pathway and plays a vital role in cancers and developmental syndromes (RASopathies). The current review discusses the clinical significance of the BRAF gene and other members of RAS/RAF cascade in human cancers and...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章,评审
doi：10.1016/j.sjbs.2014.10.002
更新日期：2015-07-01 00:00:00
abstract：:Diplometopon zarudnyi, a worm lizard belongs to amphisbaenia under trogonophidae family. This species exists in limited areas of the Arabian Peninsula and is an oscillating digger found in sub-surface soils. The present study aimed to investigate the sperm tail differentiation in D. zarudnyi. Ten male adults of D. zar...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2015.05.002
更新日期：2015-07-01 00:00:00
abstract：:Metabolic syndrome (Mets) is a major health hazard. The syndrome is strongly linked to cardiovascular disease. The objective of the current study was to address whether or not crocin could protect against experimentally-induced MetS in rats as well as the possible underlying mechanisms. Animals were allocated into 4 g...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.01.004
更新日期：2020-05-01 00:00:00
abstract：:Severe Acute Respiratory Syndrome Coronavirus2 (SARS-CoV2) provoked alertness globally. Existing pandemic eruption of infections with SARS-CoV2 has been phrased as coronavirus disease 2019 (covid-19). Worldwide pneumonia outburst attributable to new SARS-CoV2 alleged to be originated in Wuhan city of China and has aff...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章,评审
doi：10.1016/j.sjbs.2020.07.026
更新日期：2020-12-01 00:00:00
abstract：:Legumes are essential and play a significant role in maintaining food standards and augmenting physiochemical soil properties through the biological nitrogen fixation process. Biotic and abiotic factors are the main factors limiting legume production. Classical breeding methodologies have been explored extensively abo...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章,评审
doi：10.1016/j.sjbs.2019.11.018
更新日期：2020-01-01 00:00:00
abstract：:Three important varieties of dates (Sukri, Barhi, and Rothana), grown in Saudi Arabia were analyzed for their chemical, mineral and anti-bacterial activity. Among all the varieties analyzed, the Sukri dates had the highest moisture (16%), carbohydrate (80.65 mg/g) and the phenolic content (1.24 mg GAE/100 g) whereas, ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2019.11.029
更新日期：2020-07-01 00:00:00
abstract：:The goal of this study was to assess the antitumor efficacy and safety of lobaplatin-based regimens as the second line of treatment in patients with metastatic breast cancer (MBC) resistant to anthracyclines and taxanes, compared with that of cisplatin-based regimens. During August 2012 to April 2015, 87 patients who ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2018.01.011
更新日期：2018-07-01 00:00:00
abstract：:Methicillin-Resistant Staphylococcus aureus (MRSA) represents one of the major causes of nosocomial infections, leading to high mortality. Surfaces in clinics, as well as the attending uniform and the hands of the dental doctor can be MRSA reservoirs. Having this in mind, the purpose of this study was to evaluate the ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2019.12.003
更新日期：2020-04-01 00:00:00
abstract：:This study aimed to estimate the proximate, phenolic and flavonoids contents and phytochemicals present in seeds of twenty four soybeans (Glycine max (L.) Merr) genotypes to explore their nutritional and medicinal values. Crude protein composition ranged between 35.63 and 43.13% in Argentinian and USA (Clark) genotype...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.10.014
更新日期：2018-01-01 00:00:00
abstract：:Considering the potentiality of honey in combating diseases, the present study was carried out aiming to assess the in vitro antiprotozoal activity of several honeys (Ziziphus spina-christi, Acacia nilotica, Acacia seyal, and Cucurbita maxima) against Entamoeba histolytica and Giardia lamblia by employing the sub-cult...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.06.004
更新日期：2019-02-01 00:00:00
abstract：:Background: Propolis is a natural product of plant resins collected by honeybees from various plant sources. It is used as a remedy in folk medicine since ancient times because of its several biological and pharmacological properties. Recently, propolis has been used by dentist to treat various oral diseases. It was a...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2016.12.019
更新日期：2017-07-01 00:00:00
abstract：:Agaricus blazei Murill is one of the very popular edible medicinal mushrooms. The present study investigated the protective effect of this biologically active mushroom on the tissue peroxidative damage and abnormal antioxidant levels in carbon tetrachloride induced hepatotoxicity in male albino rats. Male albino rats ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2012.03.004
更新日期：2012-07-01 00:00:00
abstract：:Arbuscular mycorrhizal fungi (AMF) inoculation and biochar amendment has been reported to improve growth of several crop plants however their role in stress amelioration individually as well as in combination has not been worked out. This experiment was conducted to evaluate the application of AMF and biochar on the p...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2018.11.005
更新日期：2019-03-01 00:00:00
abstract：:A field experiment was conducted to investigate the effect of seed presoaking of shikimic acid (30, 60 and 120 ppm) on growth parameters, fruit productivity and quality, transpiration rate, photosynthetic pigments and some mineral nutrition contents of tomato plants. Shikimic acid at all concentrations significantly i...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2013.03.002
更新日期：2013-10-01 00:00:00
abstract：:This study aims to present an integrated process that can be used to produce biomedical and biological active components from the fruit shell of Camellia oleifera Abel. Through the Foss method, Aldehyde, acid compounds, acyl and alcohol compounds account for 22.7, 15.93, 0.24 and 61.13% of the extractives which were e...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.08.006
更新日期：2018-02-01 00:00:00
abstract：:Conservation in the Kingdom of Saudi Arabia is relatively young, yet have made considerable gains in conservation through strategic proclamation and reintroductions. Changes in land use, illegal hunting and competition with domestic stock has decimated the native ungulates, meaning that the survival of the native ungu...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.03.009
更新日期：2018-02-01 00:00:00
abstract：:Tomato (Lycopersicon esculentum) is one of the widely grown vegetables worldwide. Fusarium oxysporum f. sp. lycopersici (FOL) is the significant contributory pathogen of tomato vascular wilt. The initial symptoms of the disease appear in the lower leaves gradually, trail by wilting of the plants. It has been reported ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章,评审
doi：10.1016/j.sjbs.2019.06.002
更新日期：2019-11-01 00:00:00
