Tryptase does not alter transepithelial conductance or paracellular permeability in human airway epithelial cells.

Abstract:

BACKGROUND:Cell tight junction proteins create a barrier between airway epithelial cells to limit paracellular transport from the apical to basolateral surface. This barrier can impede the entry of respiratory pathogens and toxins from the airway lumen into the systemic circulation. Mast cell-mediated inflammation in the human airway can cause a disruption of this barrier. Tryptase is one of the major mediators released by mast cells and has been studied extensively in diseases such as asthma, reflux, and sinusitis. We hypothesize that tryptase may play a role in airway paracellular permeability by disrupting the cell tight junction proteins. METHODS:We tested this hypothesis by applying tryptase on the apical and basolateral surface to primary human airway epithelia grown in an air-liquid interface and measured changes in the transepithelial conductance and paracellular permeability of the membrane during short (every minute) and longer (over hours) time courses. We then immunostained the cell membranes for occludins and claudins to observe for changes in the structure of the tight junctions after tryptase application. RESULTS:Our data show that tryptase does not alter paracellular permeability in human airway cells over minutes or hours, and that tryptase does not alter the structure of the cell junction. CONCLUSION:Tryptase alone does not alter paracellular permeability in human airway cells. Tryptase may be altering the epithelial membrane independent of the cell tight junction pathway or other mast cell mediators may play a role in permeability.

journal_name

Am J Rhinol Allergy

authors

Chang EH,Lee JH,Zabner J

doi

10.2500/ajra.2010.24.3442

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

126-8

issue

2

eissn

1945-8924

issn

1945-8932

journal_volume

24

pub_type

杂志文章
  • The liquid Panax ginseng inhibits epidermal growth factor-induced metalloproteinase 9 and cyclooxygenase 2 expressions via inhibition of inhibitor factor kappa-B-alpha and extracellular signal-regulated kinase in NCI-H292 human airway epithelial cells.

    abstract:BACKGROUND:Ginseng (Panax ginseng C.A. Meyer) has been used in Asian countries for the treatment of various diseases. However, the mechanisms of liquid Panax ginseng (LG) on allergic inflammatory response in epidermal growth factor (EGF)-stimulated human airway epithelial cells remain largely unclear. METHODS:MUC5AC, ...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2011.25.3586

    authors: Kim JH,Kang JW,Kim M,Lee DH,Kim H,Choi HS,Kim EJ,Chung IM,Chung IY,Yoon DY

    更新日期:2011-03-01 00:00:00

  • Nasal and exhaled nitric oxide in chronic rhinosinusitis with polyps.

    abstract:BACKGROUND:The ciliary epithelial cells in the paranasal sinuses produce nasal nitric oxide (NO) continuously and plays a variety of roles in the paranasal sinuses. The purpose of this study was to assess whether we can use the levels of nasal NO (nNO) and exhaled NO (eNO) as a tool for evaluation in chronic rhinosinus...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2014.28.3984

    authors: Jeong JH,Yoo HS,Lee SH,Kim KR,Yoon HJ,Kim SH

    更新日期:2014-01-01 00:00:00

  • The radiologic evaluation of osteitis type and formation in chronic rhinosinusitis with and without nasal polyposis.

    abstract:OBJECTIVE:To evaluate the amount and type of osteitis observed in chronic rhinosinusitis with nasal polyposis (CRSwNP) and compare it with that of chronic rhinosinusitis without nasal polyposis (CRSnNP). SETTING:Sisli Etfal Research and Education Hospital, Istanbul, Turkey. STUDY DESIGN:Prospective, single-blind, coh...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章,随机对照试验

    doi:10.2500/ajra.2015.29.4240

    authors: Emre IE,Celebi I,Ercan I

    更新日期:2015-11-01 00:00:00

  • Role of adenosine monophosphate-activated protein kinase on cell migration, matrix contraction, and matrix metalloproteinase-1 and matrix metalloproteinase-2 production in nasal polyp-derived fibroblasts.

    abstract:PURPOSE:Activation of adenosine monophosphate-activated protein kinase (AMPK) by metformin, as a master regulator of metabolism, is involved in airway tissue remodeling. Here, we investigated the physical role of AMPK on cell migration, matrix contraction, and the production of matrix metalloproteinases (MMP) in nasal ...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2017.31.4477

    authors: Um JY,Lee SA,Park JH,Shin JM,Park IH,Lee HM

    更新日期:2017-11-01 00:00:00

  • Endoscopic endonasal resection of esthesioneuroblastoma: a multicenter study.

    abstract:BACKGROUND:This study reports the combined experience of the University of Miami and University of Pittsburgh with endoscopic endonasal resection of esthesioneuroblastoma (ENB). A retrospective case series review was performed in a tertiary care university hospital. METHODS:Twenty-three patients, 16 men and 7 women, w...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章,多中心研究

    doi:10.2500/ajra.2009.23.3269

    authors: Folbe A,Herzallah I,Duvvuri U,Bublik M,Sargi Z,Snyderman CH,Carrau R,Casiano R,Kassam AB,Morcos JJ

    更新日期:2009-01-01 00:00:00

  • Efficacy of lidocaine with or without epinephrine in rigid nasal endoscopy.

    abstract:BACKGROUND:This study evaluates if there is any benefit of adding epinephrine to lidocaine in patients undergoing diagnostic rigid nasal endoscopy. A prospective, randomized, double-blinded study was performed. METHODS:Seventy patients were randomized to receive either 2% lidocaine or 2% lidocaine with 0.2% epinephrin...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章,随机对照试验

    doi:10.2500/ajra.2014.28.4110

    authors: Al-Qudah M

    更新日期:2014-11-01 00:00:00

  • Changes of airflow pattern in inferior turbinate hypertrophy: a computational fluid dynamics model.

    abstract:BACKGROUND:Nasal obstruction (NO) is a very common symptom, but its effect on nasal physiology has not been fully understood. We performed this study to determine the effect of severity of NO due to inferior turbinate hypertrophy on airflow pattern using the computational fluid dynamics simulations. METHODS:A three-di...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2009.23.3287

    authors: Lee HP,Poh HJ,Chong FH,Wang de Y

    更新日期:2009-03-01 00:00:00

  • Prognostic factors for survival in patients with acute invasive fungal rhinosinusitis.

    abstract:BACKGROUND:Acute invasive fungal rhinosinusitis (AIFR) is an aggressive opportunistic infection with a high mortality rate. There are few reports that demonstrate an improvement in the overall prognosis. Furthermore, definite prognostic factors related to patient survival remain unclear. OBJECTIVE:The objective of thi...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2015.29.4115

    authors: Cho HJ,Jang MS,Hong SD,Chung SK,Kim HY,Dhong HJ

    更新日期:2015-01-01 00:00:00

  • New adenylate kinase 7 (AK7) mutation in primary ciliary dyskinesia.

    abstract:BACKGROUND:Primary ciliary dyskinesia (PCD) is a congenital hereditary disease affecting 1/20,000-60,000 people that causes chronic sinusitis, bronchiectasis, sinus hypoplasia, secretory otitis media, and low fertility. The complexity and heterogeneity of the disease make diagnosis difficult. Although the genetic orig...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2012.26.3784

    authors: Mata M,Lluch-Estellés J,Armengot M,Sarrión I,Carda C,Cortijo J

    更新日期:2012-07-01 00:00:00

  • Impact of endoscopic sinus surgery on olfaction and use of alternative components in odor threshold measurement.

    abstract:BACKGROUND:One of the major causes of olfactory disturbances is chronic rhinosinusitis (CRS), and the main surgical modality to treat CRS is functional endoscopic sinus surgery (FESS). It, therefore, is essential to assess the effect of this surgery on olfaction. Also, it is necessary to find new ways of measuring olfa...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2015.29.4207

    authors: Gupta D,Gulati A,Singh I,Tekur U

    更新日期:2015-07-01 00:00:00

  • Outcomes after endoscopic sinus surgery for unilateral versus bilateral chronic rhinosinusitis with nasal polyposis.

    abstract:BACKGROUND:Endoscopic sinus surgery (ESS) is a safe and reliable procedure for the treatment of chronic rhinosinusitis (CRS) and nasal polyposis (NP). Although most cases of CRS with NP occur bilaterally, we occasionally encounter patients with unilateral sinus disease with NP. Because no study has been conducted on th...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2010.24.3482

    authors: Lee JY,Byun JY,Shim SS,Lee SW

    更新日期:2010-05-01 00:00:00

  • Variability in Retronasal Odor Identification Among Patients With Chronic Rhinosinusitis.

    abstract::Background Retronasal olfaction is important in flavor detection and enjoyment. The ability to identify specific individual retronasal odors may play a role in quality of life for patients with chronic rhinosinusitis (CRS). Objective To identify patterns and improve understanding of retronasal identification of indivi...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.1177/1945892418793540

    authors: Ganjaei KG,Soler ZM,Storck KA,Rowan NR,Othieno FA,Schlosser RJ

    更新日期:2018-09-01 00:00:00

  • A comparison of the psychometric properties of the Mini-Rhinitis Quality of Life Questionnaire and the Rhinitis Control Assessment Test.

    abstract:BACKGROUND:The relationships between validated quality of life tools and control tools have not been explored for rhinitis. This study was designed to determine relationships of the Mini-Rhinitis Quality of Life Questionnaire (MiniRQLQ) and the Rhinitis Control Assessment Test (RCAT) to each other regarding content an...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2012.26.3744

    authors: Schatz M,Zeiger RS,Chen W,Yang SJ,Stanford RH,Garris CP

    更新日期:2012-03-01 00:00:00

  • Impact of Middle Turbinectomy on Airflow to the Olfactory Cleft: A Computational Fluid Dynamics Study.

    abstract:BACKGROUND:The impact of middle turbinate resection (MTR) on olfaction remains a point of debate in the current literature. Few studies have objectively evaluated olfactory cleft airflow following MTR; thus, the mechanism by which MTR may impact olfaction is poorly understood. It is not known whether the postsurgical c...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.1177/1945892418816841

    authors: Alam S,Li C,Bradburn KH,Zhao K,Lee TS

    更新日期:2019-05-01 00:00:00

  • Endoscopic transnasal transethmoid ligation of the anterior ethmoid artery.

    abstract:BACKGROUND:The current otolaryngologic literature is lacking in basic science investigation regarding endoscopic anterior ethmoid artery (AEA) ligation as part of the armamentarium for epistaxis control. This investigation provides the anatomic rationale for endoscopic ligation of the AEA in the surgical treatment of e...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2009.23.3295

    authors: Camp AA,Dutton JM,Caldarelli DD

    更新日期:2009-03-01 00:00:00

  • The effect of topical FK506 (tacrolimus) in a mouse model of allergic rhinitis.

    abstract:BACKGROUND:The management of allergic rhinitis (AR) encompasses education, pharmacotherapy, immunotherapy, and surgery. FK506 (tacrolimus) is an immunosuppressant that inhibits allergic reactions. The purpose of this study was to reveal whether FK506 treatment reduces allergic inflammation in an AR mouse model and to ...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2012.26.3743

    authors: Shin JH,Park HR,Kim SW,Park CS,Cho JH,Park YJ,Kim SW

    更新日期:2012-03-01 00:00:00

  • Novel Radiographic Assessment of the Cribriform Plate.

    abstract::Background The cribriform plate (CP) is a common site of spontaneous cerebrospinal fluid (SCSF) leaks. Radiographic assessment of the anterior and lateral skull base has shown thinner bone in patients with SCSFs; however, prior assessment of the CP has required postmortem cadaver dissection. Objective To develop novel...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.1177/1945892418768159

    authors: Ganjaei KG,Soler ZM,Mappus ED,Taylor RJ,Worley ML,Mulligan JK,Mattos JL,Rowan NR,Garcia GJM,Dubno JR,Eckert MA,Matthews LJ,Schlosser RJ

    更新日期:2018-05-01 00:00:00

  • Development of the McGill simulator for endoscopic sinus surgery: a new high-fidelity virtual reality simulator for endoscopic sinus surgery.

    abstract:BACKGROUND:The technical challenges of endoscopic sinus surgery (ESS) and the high risk of complications support the development of alternative modalities to train residents in these procedures. Virtual reality simulation is becoming a useful tool for training the skills necessary for minimally invasive surgery; howeve...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2014.28.4046

    authors: Varshney R,Frenkiel S,Nguyen LH,Young M,Del Maestro R,Zeitouni A,Tewfik MA,National Research Council Canada.

    更新日期:2014-07-01 00:00:00

  • The development of allergic rhinitis in children previously diagnosed as nonallergic rhinitis.

    abstract:BACKGROUND:Nonallergic rhinitis (NAR) is characterized by nasal symptoms similar to allergic rhinitis (AR) without an IgE-mediated immune response. Limited data are available on the natural history of NAR in its progression toward AR, particularly in children. This study evaluates the development of AR in children who...

    journal_title:American journal of rhinology & allergy

    pub_type: 临床试验,杂志文章

    doi:10.2500/ajra.2013.27.3839

    authors: Veskitkul J,Vichyanond P,Visitsunthorn N,Jirapongsananuruk O

    更新日期:2013-01-01 00:00:00

  • Diagnostic and staging accuracy of magnetic resonance imaging for the assessment of sinonasal disease.

    abstract:BACKGROUND:Concerns over radiation exposure with paranasal sinus computed tomography (CT) have recently evolved. Magnetic resonance imaging (MRI) may be more advantageous in this regard, but the diagnostic assessment of MRI in chronic rhinosinusitis (CRS) has not been reported. The purpose of this study was to determin...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章,随机对照试验

    doi:10.2500/ajra.2009.23.3260

    authors: Lin HW,Bhattacharyya N

    更新日期:2009-01-01 00:00:00

  • Nanofiltered C1 esterase inhibitor for treatment of laryngeal attacks in patients with hereditary angioedema.

    abstract:BACKGROUND:Laryngeal edema is a life-threatening manifestation of hereditary angioedema (HAE), an autosomal-dominant disorder caused by quantitative or functional C1 esterase inhibitor (C1 INH) deficiency. The preparation of nanofiltered C1 INH (C1 INH-nf) used in this study is indicated for routine prophylaxis against...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2013.27.3973

    authors: Riedl MA,Lumry WR,Li HH,Craig TJ,Fitts D,Kalfus I,Uknis ME

    更新日期:2013-11-01 00:00:00

  • Nasal nitric oxide and nasal eosinophils decrease with levocetirizine in subjects with perennial allergic rhinitis.

    abstract:BACKGROUND:Allergic rhinitis is commonly treated with antihistamines. Monitoring improvement of airway inflammation noninvasively using nasal nitric oxide (nNO) would be clinically useful. To determine the anti-inflammatory effect of oral levocetirizine dihydrochloride (LC), we measured nNO and nasal eosinophils (nEos...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章,随机对照试验

    doi:10.2500/ajra.2011.25.3668

    authors: Bautista AP,Eisenlohr CP,Lanz MJ

    更新日期:2011-11-01 00:00:00

  • Prevalence and Clinical Significance of Radiographic Sinus Disease on Preoperative Computed Tomography Imaging in the Endoscopic Skull Base Surgery Population.

    abstract:INTRODUCTION:Endoscopic skull base surgery (ESBS) provides a safe, minimally invasive approach to treat benign and malignant skull base lesions. The significance of concomitant sinonasal pathology on imaging in patients undergoing ESBS and its effect on perioperative management is not well described. We sought to ident...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.1177/1945892420949130

    authors: Shah J,Cappello ZJ,Roxbury C,Tang D,Woodard TD,Kshettry VR,Recinos PF,Sindwani R

    更新日期:2020-08-11 00:00:00

  • Chapter 14: Nonallergic rhinitis.

    abstract::Rhinitis is characterized by one or more of the following nasal symptoms: congestion, rhinorrhea (anterior and posterior), sneezing, and itching. It is classified as allergic or nonallergic, the latter being a diverse syndrome that is characterized by symptoms of rhinitis that are not the result of IgE-mediated events...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2013.27.3927

    authors: Settipane RA,Kaliner MA

    更新日期:2013-05-01 00:00:00

  • Management of sinonasal inverted papillomas and comparison of classification staging systems.

    abstract:BACKGROUND:The aim of this study was to describe treatment results in patients with sinonasal inverted papilloma and to compare recent classification staging systems. METHODS:We performed a retrospective study of 79 patients with primary paranasal sinus inverted papilloma from 1978 to 2008. RESULTS:The median age at ...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2010.24.3421

    authors: Gras-Cabrerizo JR,Montserrat-Gili JR,Massegur-Solench H,León-Vintró X,De Juan J,Fabra-Llopis JM

    更新日期:2010-01-01 00:00:00

  • The Candy Smell Test in clinical routine.

    abstract:BACKGROUND:The "Candy Smell Test" (CST) has been introduced as a new testing method for the evaluation of the human sense of smell. In contrast to other established orthonasal smell tests, the CST addresses the retronasal application of odors, typical for food aroma effects during mastication and swallowing. The aim of...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2011.25.3611

    authors: Haxel BR,Bertz-Duffy S,Faldum A,Trellakis S,Stein B,Renner B,Kobal G,Letzel S,Mann WJ,Muttray A

    更新日期:2011-07-01 00:00:00

  • The feasibility of 18F-fluorodeoxyglucose-positron emission tomography uptake as a prognostic factor for paranasal sinus malignancy.

    abstract:BACKGROUND:There is a correlation between (18)F-fluorodeoxyglucose (FDG) uptake and the level of intracellular metabolic tumor activity, which in turn may be associated with active proliferation, invasion, and prognosis as well as distant metastases in head and neck cancer. This study was designed to assess whether tum...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2013.27.3848

    authors: Seol JH,Kim J,Lee JD,Lee JG,Yoon JH,Kim CH

    更新日期:2013-03-01 00:00:00

  • Aberrant mucin glycoprotein patterns of chronic rhinosinusitis patients with bacterial biofilms.

    abstract:BACKGROUND:Increasingly bacterial biofilms have been implicated in chronic rhinosinusitis (CRS), and conventional treatment methods have failed to completely eradicate biofilms. (1) Terminal sialic acids present on sinus mucosal glycoproteins are targets for bacterial adherence and biofilm formation. (2) A subpopulatio...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2010.24.3504

    authors: Tan L,Psaltis A,Baker LM,McGuckin M,Rousseau K,Wormald PJ

    更新日期:2010-09-01 00:00:00

  • Characterization of bacterial and fungal biofilms in chronic rhinosinusitis.

    abstract:BACKGROUND:Conclusive evidence exists that biofilms are present on the mucosa of chronic rhinosinusitis (CRS) patients. Less is known about the species constituting these biofilms. This study developed a fluorescence in situ hybridization (FISH) protocol for characterization of bacterial and fungal biofilms in CRS. ME...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2009.23.3413

    authors: Foreman A,Psaltis AJ,Tan LW,Wormald PJ

    更新日期:2009-11-01 00:00:00

  • Endoscopic septoplasty: "How I do it".

    abstract:BACKGROUND:Multiple methods exist for nasal septoplasty. Proliferation of endoscopic technology has broadened these options. METHODS:Technical description of two techniques for endoscopic septoplasty. RESULTS:The endoscope can be utilized for visualization under the flaps after, a Killian or hemitransfixion incision,...

    journal_title:American journal of rhinology & allergy

    pub_type: 杂志文章

    doi:10.2500/ajra.2017.31.4436

    authors: Chandra RK

    更新日期:2017-07-01 00:00:00