Abstract:
OBJECTIVE:The aim of this study was to explore the effect of micro ribonucleic acid (miR)-210 on neuronal apoptosis in rats with cerebral infarction (CI) by regulating the vascular endothelial growth factor (VEGF)-Notch signaling pathway. MATERIALS AND METHODS:A total of 30 clean healthy male Sprague-Dawley rats weighing 200-300 g were selected and randomly divided into Sham group (n=10), CI model group (CIM group, n=10), and CIM + miR-210 Mimic group (n=10). The protein expression levels of VEGF, Notch1, cleaved-Caspase3 (c-Caspase3), B-cell lymphoma-2 (Bcl-2), and tubulin were detected via Western blotting. The messenger RNA (mRNA) levels of VEGF and Notch1 were detected via quantitative Polymerase Chain Reaction (qPCR). Meanwhile, the expression levels of VEGF and Notch1 in tissues were determined using immunohistochemistry. Furthermore, the apoptosis of tissues was determined via Annexin V-FITC, propidium iodide (PI) double labeling, and flow cytometry. RESULTS:The levels of VEGF and Notch1 increased significantly in the CIM group when compared with those in the Sham group (p<0.01). However, their expressions decreased remarkably in CIM + miR-210 Mimic group when compared with CIM group (p<0.05). The mRNA expressions of VEGF and Notch1 were evidently upregulated in the CIM group when compared with the Sham group (p<0.01), whereas they were remarkably downregulated in the CIM + miR-210 Mimic group than CIM group (p<0.05). Immunohistochemistry results indicated that the expression levels of VEGF and Notch1 in tissues were consistent with Western blotting results. Besides, the protein expressions of c-Caspase3 and Bcl-2 were remarkably higher in the CIM group than Sham group (p<0.01). However, they were significantly lower in the CIM + miR-210 Mimic group than those in the CIM group (p<0.05). In addition, flow cytometry results demonstrated that the apoptosis level increased significantly in CIM group when compared with the Sham group (p<0.05), while it was remarkably inhibited in the CIM + miR-210 Mimic group (p<0.05). CONCLUSIONS:MiR-210 can reduce the protein expressions of VEGF and Notch1, inhibit the VEGF-Notch signaling pathway, decrease the expression of pro-apoptotic factor c-Caspase3 and increase the expression of anti-apoptotic factor Bcl-2, thereby suppressing cerebral neuronal apoptosis and preventing CI-induced neuronal apoptosis.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Jiang YL,Liu WW,Wang Y,Yang WYdoi
10.26355/eurrev_202005_21188subject
Has Abstractpub_date
2020-05-01 00:00:00pages
4971-4978issue
9eissn
1128-3602issn
2284-0729journal_volume
24pub_type
杂志文章abstract:OBJECTIVE:The occurrence rate of delayed fracture healing in diabetes mellitus patients is high. Transforming growth factor β1 (TGF-β1) is an important regulatory factor in bone tissue repair and regeneration. However, TGF-β1 expression and its function in diabetic patient fracture have not been fully elucidated. PATI...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201801_16064
更新日期:2018-10-01 00:00:00
abstract::Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, "MiR-182 affects renal cancer cell proliferation, apoptosis, and invasion by regulating PI3K/AKT/mTOR signaling pathway, by J.-H. Fu, S. Yang, C.-J. Nan, C...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202007_21852
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVE:The previous work has shown that Berberine and Hesperidin have beneficial effects on cardiovascular diseases. However, the underlying mechanisms remain unknown. This study aimed to investigate the effect of Berberine and Hesperidin on inflammatory cytokine secretion, proliferation, differentiation, and collag...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201906_18216
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to explore the regulatory effects of micro ribonucleic acid (miR)-376b-3p on proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells by targeting Kruppel-like factor 15 (KLF15) and its mechanism of action. PATIENTS AND METHODS:The expression of miR-376b-3p in NSCLC a...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202009_23033
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to explore the effects of micro ribonucleic acid (miR)-506 and phosphatidylinositol 3-hydroxy kinase (PI3K)/protein kinase B (AKT) pathway on myocardial ischemia-reperfusion injury (MIRI) in rats. MATERIALS AND METHODS:A total of 90 healthy rats weighing 260-300 g were selected as r...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202012_24193
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:Multiple studies have unveiled that long non-coding RNAs (lncRNAs) contribute to oncogenesis. LncRNA ARAP1 antisense RNA 1 (ARAP1-AS1) has been demonstrated to serve as an oncogene in bladder tumor and colorectal cancer. This study attempted to explore the correlation of ARAP1-AS1 expressions with clinical pr...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202002_20359
更新日期:2020-02-01 00:00:00
abstract::Aloe vera is a medicinal plant that promotes wound healing in burn injuries. A prospective clinical trial was conducted to evaluate the effects of a topical cream containing 0.5% Aloe vera juice powder in the treatment of chronic anal fissures. The aloe cream was applied by the patients to the wound site 3 times per d...
journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of Lactobacillus rhamnosus GR-1 on atherosclerotic progression in apolipoprotein-E knockout (ApoE-/-) mice fed with a high-fat diet and the underlying mechanisms of its action. MATERIALS AND METHODS:Eight-week-old ApoE-/- mice were treated with Lactobacillus rhamnosus GR-1 daily for...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201904_17722
更新日期:2019-04-01 00:00:00
abstract::The authors evaluated the efficacy of an electronic treatment for pain, using an ion flow generator, BE-101 model by Bio-Ejt, on 19 patients suffering from acute pain of an arthrorheumatic nature. Each patient was treated for two weeks every other day (6 sittings), each sitting lasting 20 minutes at an intensity of ab...
journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract:OBJECTIVE:Laparoscopic sleeve gastrectomy has become one of the most commonly performed bariatric operations. It is essentially a restrictive bariatric operation; however, a series of hormonal changes occurring postoperatively contribute to decreased appetite and reduced food intake. PATIENTS AND METHODS:This is a lit...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2016-12-01 00:00:00
abstract::Monitoring oxidative stress in humans is achieved by assaying products of oxidative damage or by investigating the potential of an organism, tissue or body fluids to withstand further oxidation. Unfortunately, there is little consensus concerning the selection of parameters of oxidative stress or antioxidant state to ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2007-09-01 00:00:00
abstract:OBJECTIVE:To investigate the difference between myocardial injuries caused by nonaneurysmal subarachnoid hemorrhage (SAH) or aneurysmal SAH. PATIENTS AND METHODS:A total of 92 inpatients with SAH at early stage (within 48h), who were treated in our hospital from 2008 to 2014 were enrolled in this study. Differences in...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:To explore whether miR-216a-3p could promote differentiation of bone marrow mesenchymal stem cells (BMMSCs) into type II alveolar epithelial cells (ACE II) via Wnt/β-catenin pathway, thereby alleviating neonatal respiratory distress syndrome (NRDS). MATERIALS AND METHODS:BMMSCs were directionally differentia...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201811_16410
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVE:To uncover the role of UPF1 in alleviating the progression of glioma via targeting long non-coding RNA (lncRNA) CYTOR and underlying mechanism. PATIENTS AND METHODS:A total of 30 glioma tissues surgically resected from glioma patients and 30 brain tissues were collected from brain trauma patients undergoing ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201911_19567
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVE:Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed cancers globally. LncRNA HLA complex group 11 (HCG11) has been reported to play an oncogenic role in multiple cancers. Nevertheless, the role and regulatory mechanism of HCG11 in HCC are not fully addressed. PATIENTS AND METHODS:The abundan...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201912_19771
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVE:The etiology of Major depressive disorder (MDD) is multifactorial but the genetic risk is an important factor. Previous studies have shown a significant interaction between serotonin and brain-derived neurotrophic factor (BDNF) in brain function. The serotonin transporter protein promoter polymorphism (5-HTTL...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-05-01 00:00:00
abstract:OBJECTIVES:Cardiovascular changes during laparoscopic surgery have been described in several studies. Pneumoperitoneum effects on cardiac performance instead have not been much investigated and are less known. The carbon dioxide insufflation necessary in order to perform laparoscopic procedures represents a higher forc...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-08-01 00:00:00
abstract::NLRP3 (NOD-, LRR- and pyrin domain-containing protein 3) inflammasome has recently become an intriguing target of several chronic and viral diseases. Here, we argue that targeting NLRP3 inflammasome could be a strategy to prevent cardiovascular outcomes [fulminant myocarditis, heart failure, venous thromboembolism (VT...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202009_22867
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVE:Observe the expression and distribution of HMGB1 in Sombati's cell model and kainic acid-induced epileptic rats' model. MATERIALS AND METHODS:Dissociated hippocampal neurons from neonatal SD rats and cultured those for 9 days, then changed medium to Mg2+-free medium for 3 hours to induce Sombati's cell model...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-08-01 00:00:00
abstract:OBJECTIVE:Vorinostat is a drug used to treat cutaneous T cell lymphoma whose action mechanism is based on Histone Deacetylase inhibition. Histone Deacetylases are a family of enzymes that remove acetyl groups from histone and non-histone proteins that control many crucial processes, such as gene regulation, cell cycle ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202007_21909
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVES:Acute pancreatitis (AP) is an inflammatory disease of the pancreas characterized by local inflammation. Secretory phospholipases A-II (sPLA2-II) have been implicated in triggering AP, but their exact role to evoke AP is largely unknown. NF-kB activation has previously been shown to induce acute pancreatitis....
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to explore the relationship between the mitogen-activated protein kinase (MAPK)/extracellular regulated protein kinase (ERK) pathway and neurocyte apoptosis after cerebral infarction in rats. MATERIALS AND METHODS:Neural stem cells were isolated from rats by establishing the cerebra...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201906_18206
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Leishmania parasites are intracellular haemoflagellates that infect macrophages of the skin and viscera to produce diseases in their vertebrates hosts. Antileishmania therapy is based on pentavalent antimony compounds which toxicity of these agents and the persistence of side effects are sever...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-12-01 00:00:00
abstract::The pathogenesis of diabetes mellitus wounds is complicate, and there lacks effective treatment strategies. Mesenchymal stem cells can promote wound healing. Compared with bone marrow mesenchymal stem cells, umbilical cord mesenchymal stem cells have obvious advantages in biological property. Wnts are potent regulator...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:LncRNAs participate in the proliferation, apoptosis, and invasion of colorectal cancer. We aimed at investigating the uncovered effect of lncRNALUADT1 on colorectal cancer. PATIENTS AND METHODS:The relative expression level of lncRNALUADT1 in tumor specimen was tested by Real-time quantitative PCR. The assoc...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201804_14816
更新日期:2018-04-01 00:00:00
abstract:AIM:The aim of this study was to screen miRNAs related to different subtypes of breast cancer and their target genes to identify new markers of tumor subtype. MATERIALS AND METHODS:The miRNA expression profiles of breast cancer GSE38867 including 7 ductal carcinoma in situ breast (DCIS) cancer samples, 7 invasive brea...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-10-01 00:00:00
abstract:OBJECTIVE:To investigate whether hypoxia microenvironment induced hepatocellular carcinoma cells SMMC-7721 epithelial-mesenchymal transition (EMT) and to explore the underlying molecular mechanism. MATERIALS AND METHODS:In this study, SMMC-7721 cells were cultured under normoxia and hypoxia conditions, respectively. R...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-07-01 00:00:00
abstract:OBJECTIVE:Colorectal cancer (CRC) is a common human malignancy and is the second leading cause of cancer deaths worldwide with a dismal prognosis. Previous investigations have shown that miR-340 can modulate the metabolism of CRC cells. The aim of this report is to study the role of miR-340 in the development and progr...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-06-01 00:00:00
abstract:OBJECTIVE:On December 8, 2019, many cases of pneumonia with unknown etiology were first reported in Wuhan, China, subsequently identified as a novel coronavirus infection aroused worldwide concern. As the outbreak is ongoing, more and more researchers focused interest on the COVID-19. Therefore, we retrospectively anal...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202003_20712
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVE:As an important factor regulating the epithelial mesenchymal transition (EMT) Snail is associated with lung cancer. Bioinformatics analysis showed that microRNA-30a (miR-30a) may target the 3'-UTR of Snail mRNA. It was exhibited that miR-30a down-regulation was related to tumor size, TNM stage, and poor progn...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-06-01 00:00:00