Abstract:
OBJECTIVE:The aim of this study was to explore the relationship between the mitogen-activated protein kinase (MAPK)/extracellular regulated protein kinase (ERK) pathway and neurocyte apoptosis after cerebral infarction in rats. MATERIALS AND METHODS:Neural stem cells were isolated from rats by establishing the cerebral infarction model and sham model. Isolated cells were cultured in complete culture medium in vitro. Real-time quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) was used to detect the messenger ribonucleic acid (mRNA) expression of ERK1 and ERK2 in the MARK pathway. Western blotting was applied to examine the activation of the MAPK/ERK pathway and neuron-specific markers. The expression of neuron-specific enolase (NSE) was detected via immunofluorescence. Cell activity and apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry, respectively. RESULTS:The mRNA expressions of ERK1 and ERK2 in neural stem cells increased in a time-dependent manner after cerebral infarction in rats. The expressions of ERK1, ERK2, cyclin D1, Nestin, NSE and glial fibrillary acidic-protein (GFAP) in neural stem cells were significantly decreased after being treated with SCH772984. Cell activity, proliferation and differentiation were markedly inhibited. However, cleaved-caspase 3 protein and apoptosis rate were remarkably increased. CONCLUSIONS:The MAPK/ERK pathway seriously affects neurocyte apoptosis after cerebral infarction in rats. When the MAPK/ERK pathway is inhibited, neurocyte apoptosis is remarkably increased after cerebral infarction in rats.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Wang QC,Lu L,Zhou HJdoi
10.26355/eurrev_201906_18206subject
Has Abstractpub_date
2019-06-01 00:00:00pages
5374-5381issue
12eissn
1128-3602issn
2284-0729journal_volume
23pub_type
杂志文章abstract:OBJECTIVE:To investigate the role of phosphatidylinositol-3-kinase protein kinase B (PI3K/Akt) signaling pathway in the apoptosis of H1299 lung cancer cells induced by epigallocatechin gallate (EGCG). MATERIALS AND METHODS:H1299 lung cancer cells were treated with EGCG at a dose of 10 µM, 20 µM, and 40 µM, respectivel...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201807_15511
更新日期:2018-07-01 00:00:00
abstract:OBJECTIVE:In the last decades, several cognitive-enhancing drugs have been sold onto the drug market. Methylphenidate and analogs represent a sub-class of these new psychoactive substances (NPS). We aimed to review the use and misuse of methylphenidate and analogs, and the risk associated. Moreover, we exhaustively rev...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:10.26355/eurrev_201901_16741
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to explore the influences of micro ribonucleic acid (miR)-708 on cerebral ischemia-reperfusion injury by regulating a disintegrin and metalloprotease 17 (ADAM17) in a targeted manner. MATERIALS AND METHODS:The rat model of middle cerebral artery occlusion (MCAO) was established,...
journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_202011_23835
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVE:Prostate cancer is the most frequently diagnosed cancer in men, as well as the second leading cause of death among cancers after lung cancer. In the United States, it is more prevalent in African-American men than in Caucasian men. Prostate cancer frequently metastasizes to the bone, with most of the reported...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-12-01 00:00:00
abstract:OBJECTIVE:Glioma is one common intracranial malignancy. Recently, there has been a large volume of published studies describing the functions of microRNAs as potential diagnostic markers for glioma. Data from several sources revealed that miR-378 played crucial roles in multiple tumors. However, much uncertainty still ...
journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_201806_15268
更新日期:2018-06-01 00:00:00
abstract:OBJECTIVE:Skeletal diseases, both degenerative and secondary to trauma, infections or tumors, represent an ideal target for regenerative medicine and in the last years, stem cells have been considered as good candidates for in vitro and in vivo bone regeneration. To date, several stem cell sources, such as adult mesenc...
journal_title:European review for medical and pharmacological sciences
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doi:
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVE:To investigate the safety and effectiveness of the application of high-volume hemofiltration (HVHF) in children with sepsis combined with acute kidney injury. PATIENTS AND METHODS:A total of 76 child patients were enrolled and randomly divided equally (n=38) into control and the observation groups respective...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,随机对照试验
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更新日期:2018-02-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_201909_18826
更新日期:2019-09-01 00:00:00
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更新日期:2019-08-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-10-01 00:00:00
abstract::Antinuclear antibodies (ANA) represent the main diagnostic markers for systemic autoimmune disease (AD), although their presence can be detected in blood donors. The aim of this study was to study ANA prevalence in healthy subjects of different racial groups, exposed to the same environmental factors, in order to unde...
journal_title:European review for medical and pharmacological sciences
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doi:
更新日期:2008-07-01 00:00:00
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pub_type: 临床试验,杂志文章
doi:
更新日期:2014-01-01 00:00:00
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doi:10.26355/eurrev_201801_14099
更新日期:2018-01-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_201911_19437
更新日期:2019-11-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_202004_20837
更新日期:2020-04-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-12-01 00:00:00
abstract:OBJECTIVE:Chemoresistance is the principal reason for poor survival and disease recurrence in osteosarcoma patients. Survivin, a family member of the inhibitor of apoptosis proteins, plays an important role in inhibition of apoptosis. Survivin is expressed in a vast majority of human cancers, which is often correlated ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:Risk factors for implant therapy are represented by all general and local conditions that through various mechanisms can increase either short-term and long-term failure risk. The aim of this study is to assess the implant survival and implant success rates with single and multiple risk factors. PATIENTS AND...
journal_title:European review for medical and pharmacological sciences
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doi:
更新日期:2017-02-01 00:00:00
abstract:OBJECTIVE:We studied the influence of Dexmedetomidine on cognitive function in children during the recovery period of general anesthesia. PATIENTS AND METHODS:Ninety-three children who underwent general anesthesia were selected and randomly divided into (1) the control group, (2) the dexmedetomidine group, and (3) the...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2017-03-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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更新日期:2011-09-01 00:00:00
abstract:AIM:We aimed to investigate whether Olmesartan had an effect on cystatin C levels in hypertensive patients, and evaluate its correlation with blood pressure (BP). MATERIALS AND METHODS:Seventy-two patients essential hypertension patients with a known for, at most, the last 3 years were enrolled to the study. Patients ...
journal_title:European review for medical and pharmacological sciences
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doi:
更新日期:2011-12-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2014-01-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_202007_22226
更新日期:2020-07-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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更新日期:2018-05-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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更新日期:2019-02-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-06-01 00:00:00
abstract:OBJECTIVE:To investigate the effects of recombinant activated coagulation factor VII (rFVIIa) on apoptosis and the expressions of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) in rats with intracerebral hemorrhage (ICH). MATERIALS AND METHODS:A total of 90 8-week-old male Sprague-Dawley (SD) rats with...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201808_15725
更新日期:2018-08-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201812_16514
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVE:Obesity is an important preventable cause of death and is a major risk factor for cardiovascular diseases as well as skeletal system diseases and malignancies. In many studies, complete blood count (CBC) and ratios derived from its results - such as the neutrophil/lymphocyte ratio, platelet/lymphocyte ratio a...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-04-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
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更新日期:2020-09-01 00:00:00