Abstract:
:We have investigated the impaired secretion response of neonatal platelets. We compared the response of washed neonatal and adult platelets to thrombin and collagen, and to specific activators of calcium flux (inositol trisphosphate) and protein kinase C activation (oleoyl-acetyl glycerol). Neonatal platelets show no impairment of aggregation, secretion of [14C]serotonin or phosphorylation of specific intracellular proteins in response to thrombin, inositol trisphosphate, or oleoyl-acetyl glycerol. However, neonatal platelets have a markedly decreased response to collagen. To further evaluate this deficient response, we examined specific aspects of the collagen activation pathway. Collagen-platelet interaction as measured by adhesion of platelets to collagen-coated dishes showed no difference in adhesion of neonatal platelets compared to adult controls (20.1 +/- 11.6 versus 18.6 +/- 9.3%). The presence of GPIa/IIa, a Mg2(+)-dependent collagen receptor, was evaluated by flow cytometric analysis of binding of fluorescein-tagged monoclonal antibody, 6F1 (directed against GPIa/IIa). There was no difference either in the percent of platelets that bound antibody (80 versus 81%) or in the mean fluorescence intensity of the adult and neonatal samples. Phosphoinositide hydrolysis was decreased in neonatal platelets in response to collagen but normal in response to thrombin. Neonatal platelets released more arachidonic acid than adult platelets in response to thrombin (29.5 +/- 3.2 versus 19.6 +/- 1.8%) but less than adult platelets in response to 10 micrograms/mL collagen (3.2 +/- 1.1 versus 9.3 +/- 3.0%).(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Israels SJ,Daniels M,McMillan EMdoi
10.1203/00006450-199004000-00004subject
Has Abstractpub_date
1990-04-01 00:00:00pages
337-43issue
4 Pt 1eissn
0031-3998issn
1530-0447journal_volume
27pub_type
杂志文章abstract::Inhibition of Pseudomonas cepacia (but not Pseudomonas aeruginosa) by beta-lactams was decreased in 5% CO2 in air compared with air alone. The effect of CO2 and pH (range, 6.0 to 8.0) on beta-lactam susceptibility, beta-lactamase expression, and outer membrane proteins was studied in isolates recovered from the sputum...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199403000-00003
更新日期:1994-03-01 00:00:00
abstract:BACKGROUND:The aim was to identify susceptibility alleles for infantile hypertrophic pyloric stenosis (IHPS) in a pedigree previously linked to IHPS5 on chromosome 16q24. METHODS:We screened the positional and functional candidate gene FOXF1 by Sanger sequencing in a single affected individual. All family members for ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2016.244
更新日期:2017-04-01 00:00:00
abstract::Normal hemostasis depends in part on the balance achieved between proaggregatory and prothrombotic platelet thromboxane A2, measured as its stable end-product thromboxane B2 (TXB2), and vascular prostacyclin (PGI2), which inhibits platelet aggregation and is antithrombotic. Cystathionine-beta-synthase deficiency is ch...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198206000-00018
更新日期:1982-06-01 00:00:00
abstract::Deficiency of the lipoprotein complex, surfactant, can lead to respiratory distress syndrome (RDS) in the prematurely born infant. The surfactant proteins (SP) play important roles in the function of surfactant. Previously, we have characterized four allelic variants of the SP-A1 gene (6A, 6A2, 6A3, and 6A4) and five ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199802000-00003
更新日期:1998-02-01 00:00:00
abstract::Although fetal asphyxia, i.e. hypoxemia, acidosis, and hypercapnia, increases plasma arginine vasopressin (AVP) greater than 40-fold, hypoxemia and metabolic acidosis occurring independently cause only 5-fold and 2-fold increases, respectively. To determine the effects of hypercapnia on AVP release, we examined the ef...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199110000-00014
更新日期:1991-10-01 00:00:00
abstract::This paper has been retracted at the request of the authors. ...
journal_title:Pediatric research
pub_type: 杂志文章,撤回出版物
doi:10.1038/s41390-019-0529-y
更新日期:2019-11-01 00:00:00
abstract::Recent studies have shown 1,25(OH)2D3 receptor-mediated modulation of leukocyte proliferation, differentiation, and function. We examined the phagocytosis and killing of microorganisms by neutrophils and monocytes from five patients of three families with hereditary resistance to 1,25(OH)2D3. Phagocytosis of microorga...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198903000-00012
更新日期:1989-03-01 00:00:00
abstract::The immunological competence of neonatal rabbits inoculated intradermally with Treponema pallidum was examined. Both cellular responses and the production of humoral antibody to specific T. pallidum antigens and to nonspecific antigens or mitogens were investigated. In blast transformation assays, splenic and poplitea...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198410000-00013
更新日期:1984-10-01 00:00:00
abstract::We evaluated the hypothesis that increased endogenous opioid activity mediates part or all of the left ventricular contractile and pump dysfunction previously demonstrated in HCl-induced metabolic acidemia. Eighteen Western newborn lambs were catheterized and instrumented; pacing wires were sutured to the right atrial...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198806000-00023
更新日期:1988-06-01 00:00:00
abstract::Kinetic data are presented for the decarboxylation of branched chain alpha-ketoacids (BCKA) by intact human fibroblasts. Cultured cells of normal individuals and nine patients with different clinical pictures of maple syrup urine disease (MSUD) are studied with both alpha-ketoisocaproic acid (2-oxo-4-methylpentanoic a...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197509000-00005
更新日期:1975-09-01 00:00:00
abstract::The effects of fetal hypoxemia on blood coagulation (platelet count, prothrombin time, partial thromboplastin time, fibrinogen, factors II, V, VI, VIII, IX, X, XI, XII, XIII and von Willebrands activities, fibrin degradation products, and fibrin monomer) were evaluated in nine chronically catheterized fetal lambs earl...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198201001-00003
更新日期:1982-01-01 00:00:00
abstract::Carbohydrate and lipid intakes have both been found to modulate the metabolism of long-chain fatty acids. To define the respective influence of these two energy substrates on plasma fatty acid concentrations, 32 studies were performed in 16 parenterally fed newborn infants (mean +/- SEM, birth wt: 2.15 +/- 0.1 kg, age...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198910000-00002
更新日期:1989-10-01 00:00:00
abstract::Immunosuppressive effects of carrageenan, a high-molecular-weight polysaccharide, on antibody and T cell responses have been previously demonstrated. However, its effect on anaphylaxis is unknown. Our objectives were to test carrageenan-mediated oral tolerance induction in young mice subsequently sensitized to a commo...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200103000-00018
更新日期:2001-03-01 00:00:00
abstract::Uteroplacental insufficiency alters the anabolic metabolism of the fetus, resulting in intrauterine growth retardation (IUGR). The metabolic and physiologic factors that cause IUGR have long standing consequences after birth. Postnatal growth and glucose metabolism are altered in the IUGR infant. Skeletal muscle is an...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199805000-00001
更新日期:1998-05-01 00:00:00
abstract::The short-term (0-48 h) effects of maternal betamethasone administration on computerized fetal heart rate (FHR) parameters were studied in 36 pregnancies at increased risk for preterm delivery. FHR was recorded for 90 min immediately before the start of betamethasone treatment and again at 6-h intervals during the nex...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000155948.83570.EB
更新日期:2005-04-01 00:00:00
abstract::The relationship between cerebral blood flow (CBF) and the evolution of brain edema was investigated in an experimental model of perinatal hypoxic-ischemic brain damage. Seven-d postnatal rats were subjected to unilateral common carotid artery ligation followed by 3 h of hypoxia with 8% oxygen at 37 degrees C. This in...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199005000-00007
更新日期:1990-05-01 00:00:00
abstract::We assessed the kinetics of hyperoxia-induced prostaglandin E2 (PGE2) production by cultured rabbit tracheal epithelial (TE) cells with different inherent capacities to generate PGE2 and the role of endogenous PGE2 production in protecting these cells from hyperoxic injury. Rabbit TE cells grown to confluence with or ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199207000-00017
更新日期:1992-07-01 00:00:00
abstract::Plasma aldosterone (A), corticosterone (B), deoxycorticosterone (DOC), progesterone (P), 17-hydroxyprogesterone (17-OHP), cortisol (F), and cortisone (E) were measured simultaneously by specific radioimmunoassays in small plasma samples obtained from 174 normal infants and children between 2 hr and 15 yr of age. The s...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198001000-00010
更新日期:1980-01-01 00:00:00
abstract::Pearson's syndrome, a rare and fatal disorder characterized by refractory sideroblastic anemia and pancreatic insufficiency in infancy, is classified into mitochondrial cytopathies. To understand the molecular and genetic bases of this disorder, we have investigated the mitochondrial respiratory chain enzymes and the ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199307000-00024
更新日期:1993-07-01 00:00:00
abstract:BACKGROUND:Risk factors for diastolic dysfunction in hypertrophic cardiomyopathy (HCM) are poorly understood. We investigated the association of variants in hypoxia-response genes with phenotype severity in pediatric HCM. METHODS:A total of 80 unrelated patients <21 y and 14 related members from eight families with HC...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2012.126
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:Earlier identification of children with prenatal alcohol exposure (PAE) remains a challenge. The objective of this study was to identify neurobehavioral (NB) outcomes associated with PAE in infants. METHODS:This manuscript evaluates NB outcomes at 6.33±1.12 months of age in 93 infants (39 PAE and 54 No-PAE)...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2018.26
更新日期:2018-09-01 00:00:00
abstract::A four-year-old Israeli Arab boy was found to have glycogen accumulation in both liver and muscle without clinical symptoms. Liver phosphorylase kinase (PK) activity was 20% of normal, resulting in undetectable activity of phosphorylase a. Muscle PK activity was about 25% of normal, resulting in a marked decrease of p...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198104000-00002
更新日期:1981-04-01 00:00:00
abstract::Immune and lectin histochemical and microchemical methods were employed to study growth cartilage from seven cases of achondrogenesis type II (Langer-Saldino). The normal architecture of the epiphyseal and growth plate cartilage was replaced by a morphologically heterogeneous tissue. Some areas were comprised of vascu...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198709000-00017
更新日期:1987-09-01 00:00:00
abstract:INTRODUCTION:Current advances in neonatology have improved survival among preterm and low-birth-weight infants. However, the risk of neonatal death in preterm infants is much greater than in full-term neonates and is frequently associated with infections. METHODS:Little is known about the immune status of preterm neon...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2012.6
更新日期:2012-05-01 00:00:00
abstract::BackgroundExtracellular adenine nucleotides contribute to ischemia-reperfusion injury following infant cardiopulmonary bypass (CPB), whereas conversion to adenosine may be protective. Alkaline phosphatase (AP), a key enzyme responsible for this conversion, decreases after infant CPB. Indirect evidence suggests that so...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2017.325
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:Necrotizing enterocolitis is characterized by intestinal inflammation and epithelial barrier dysfunction. Mitogen-activated protein kinase (MAPK) phosphatase (MKP)-1 plays a pivotal role in the feedback control of MAPK signaling, which regulates inflammation and apoptosis. We hypothesized that MKP-1 prevents...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2015.88
更新日期:2015-08-01 00:00:00
abstract::Young adult macrosomic offspring of streptozotocin-induced mildly hyperglycemic rats exhibit accelerated growth through the first 10 wk of age. At 10 wk, oral glucose loading resulted in elevated plasma insulin and glucose concentrations compared to controls. To assess the mechanism of the abnormal glucose tolerance i...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199107000-00008
更新日期:1991-07-01 00:00:00
abstract::The auditory brainstem response (ABR) was monitored in nine infant rhesus monkeys during the intravenous infusion of 50-168 mg/kg of unconjugated bilirubin. Sulfisoxazole (200 mg/kg) was sometimes given near the end of or just before the bilirubin infusion if no obvious ABR change had yet occurred. Five of the animals...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198606000-00005
更新日期:1986-06-01 00:00:00
abstract::Recently a soybean oil emulsion (Intralipid) (IL) has been released in the United States for use as a parenteral nutrient. The study reported here was undertaken to determine the effect of ingestion of IL on the synthesis and secretion of the second (C2) and fourth (C4) components of complement by guinea pig peritonea...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197903000-00012
更新日期:1979-03-01 00:00:00
abstract::Wolfram syndrome 1 (WS1) is a rare autosomal recessive neurodegenerative disease characterized by diabetes insipidus, diabetes mellitus, optic atrophy, deafness, and other abnormalities. WS1 usually results in death before the age of 50 years. The pathogenesis of WS1 is ascribed to mutations of human WFS1 gene on chro...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1038/pr.2018.17
更新日期:2018-05-01 00:00:00