Abstract:
:Advanced glycation is one of the major pathophysiological posttranslational modifications. Under hyperglycemic conditions or oxidative stress, proteins and DNA are nonenzymatically modified by oxidative glycation and converted to advanced glycation endproducts (AGEs), which induce the loss of protein functions or apoptosis. This conversion to AGEs leads to the disease progression of hyperglycemia- or oxidative stress-related diseases, such as diabetic mellitus and its complications, neurodegenerative disease, atherosclerosis, and kidney disease. Further, recent evidence indicates that advanced glycation is initiated not only by oxidative stress but also by hypoxia, suggesting its pathogenesis across a wide range of diseases associated with aberrant oxygen tension as well as glucose metabolism. In addition to their role in triggering advanced glycation, these disturbances are also well known as initiators of endoplasmic reticulum (ER) stress, and of the consequent unfolded protein response (UPR). These findings strongly indicate the presence of cross talk between advanced glycation and ER stress in disease progression. In this chapter, I focus on the link between advanced glycation and ER stress, and the potential use of inhibitors of AGE formation as modulators of ER stress.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Inagi Rdoi
10.1016/B978-0-12-385928-0.00020-1subject
Has Abstractpub_date
2011-01-01 00:00:00pages
361-80eissn
0076-6879issn
1557-7988pii
B978-0-12-385928-0.00020-1journal_volume
491pub_type
杂志文章abstract::Mutations in regulators and effectors of the Rho GTPases underlie various forms of mental retardation (MR). Among them, oligophrenin-1 (OPHN1), which encodes a Rho-GTPase activating protein, was one of the first Rho-linked MR genes identified. Upon characterization of OPHN1 in hippocampal brain slices, we obtained evi...
journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/S0076-6879(07)00419-3
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abstract::This chapter described the preparation and fractionation of libraries of M13 phage displaying proteins as fusions to the major coat protein. High titer (10(13) pfu/ml) phage libraries can readily be generated using a single vector and the level of display surpasses that of gene III fusion phage. Since the synthetic VI...
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/bs.mie.2020.02.009
更新日期:2020-01-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/bs.mie.2014.11.032
更新日期:2015-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2008-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/S0076-6879(10)72015-2
更新日期:2010-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/bs.mie.2016.04.007
更新日期:2016-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/s0076-6879(02)45012-4
更新日期:2002-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/S0076-6879(08)02216-7
更新日期:2008-01-01 00:00:00
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doi:10.1016/bs.mie.2015.01.012
更新日期:2015-01-01 00:00:00
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doi:10.1016/bs.mie.2014.11.034
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
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更新日期:2013-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章,评审
doi:10.1016/bs.mie.2017.07.004
更新日期:2017-01-01 00:00:00
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
pub_type: 杂志文章,评审
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更新日期:2014-01-01 00:00:00
abstract::Embryonic stem cells (ESCs) have the ability to expand indefinitely in vitro and give rise to cells of all three germ layers as well as germ cells. For these reasons, ESCs hold great promise for biomedicine. In order to harness the potential of pluripotent cells, it is necessary to first understand the molecular mecha...
journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/S0076-6879(10)77018-X
更新日期:2010-01-01 00:00:00
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journal_title:Methods in enzymology
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doi:10.1016/S0076-6879(05)98017-8
更新日期:2005-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/S0076-6879(07)33005-X
更新日期:2007-01-01 00:00:00
abstract::Pulsed electron double resonance technique, also known as double electron-electron resonance, jointly with site-directed spin labeling (SDSL) have been used extensively for studying structures and structural change. During the last decades, significant enhancements have been made by optimization of the experimental pr...
journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/bs.mie.2015.05.027
更新日期:2015-01-01 00:00:00
abstract::We have developed two screening systems for isolating inhibitors that target bacterial two-component signal transduction: (1) a differential growth assay using a temperature-sensitive yycF mutant (CNM2000) of Bacillus subtilis, which is supersensitive to histidine kinase inhibitors, and (2) a high-throughput genetic s...
journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/S0076-6879(06)22019-6
更新日期:2007-01-01 00:00:00