Abstract:
:The foodborne trematodiases pose a significant health problem to the animals as well as the human population living in close proximities with the livestock and are still considered as the neglected tropical diseases by the World Health Organisation. The digenetic trematode, Gigantocotyle explanatum infecting the liver of Indian water buffalo, Bubalus bubalis, has been identified as one of the most common helminth parasite responsible for the disease, amphistomosis, in livestock. Despite huge abattoir prevalence, the epidemiological data and the actual economic losses incurred due to this parasite alone are yet to be established probably due to the limitations of routinely used diagnostic tests. The gold standard for the confirmation of such infections under field conditions is still the fecal egg count (FEC). However, the poor sensitivity and cumbersome nature of these tests necessitates the development of a more sensitive, reliable and easy to perform workflow/method. Immunological diagnosis of helminthic infections is still considered as an alternative to the FEC. Therefore, efforts have been made to utilize glutathione-S-transferase (GST), a vitally significant molecule of the adult G. explanatum, for the serodiagnosis of amphistomosis under both laboratory and field conditions. The GST antigen was first affinity purified from the somatic extract of the adult worms since its highest level was recorded in the somatic extracts followed by eggs and the excretory/secretory products. A five-fold affinity purified native GST antigen of about 25 kDa was found to be highly immunogenic as evident from high titre (1:25,600) of the polyclonal antibodies raised in the rabbits. The immunoblotting results revealed differential presence of GST in the adult worms, their eggs and excretory/secretory products. The immunolocalization studies revealed that the vitelline glands are the major source of GST in liver amphistome. Further, we were able to successfully screen animals naturally infected with G. explanatum using anti GST polyclonal antibodies in dot blot assay. High levels of both circulating GST antigen and anti GST antibodies were detected in the serum of the animals naturally infected with G. explanatum, while no cross reactivity was observed with the tropical liver fluke, F. gigantica which often infects the buffalo liver concurrently. The findings of the present study indicate that GST could be used as an important antigen for the diagnosis of G. explanatum infection in Indian water buffaloes.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Rehman A,Ullah R,Khan MAH,Abidi SMAdoi
10.1016/j.actatropica.2020.105400subject
Has Abstractpub_date
2020-05-01 00:00:00pages
105400eissn
0001-706Xissn
1873-6254pii
S0001-706X(19)31336-1journal_volume
205pub_type
杂志文章相关文献
ACTA TROPICA文献大全abstract::Loop-mediated isothermal amplification (LAMP) is a novel molecular method that accelerates and facilitates DNA amplification and detection under isothermal conditions. It represents a revolution in molecular biology by reducing the high cost, turnaround time and technicality of polymerase chain reaction and other ampl...
journal_title:Acta tropica
pub_type: 杂志文章,评审
doi:10.1016/j.actatropica.2012.02.004
更新日期:2012-06-01 00:00:00
abstract::Mepraia spinolai, M. gajardoi and M. parapatrica occur in distinct regions in Chile. The females of the three species are micropterous. The males of M. gajardoi are brachypterous, those of M. parapatrica can be brachypterous or macropterous and those of M. spinolai can be micropterous, brachypterous or macropterous. M...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2018.11.028
更新日期:2019-02-01 00:00:00
abstract::The pathological events in human cerebral malaria are mimicked in the experimental cerebral malaria (ECM) in Plasmodium berghei ANKA (PBA)-infected C57BL/6 mice. Although previously implied in ECM, the kinetics of cytokines and chemokines expression-an essential functional feature for defining causality in ECM develop...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2017.04.025
更新日期:2017-08-01 00:00:00
abstract::During a period of 2 years, the ecology of Bulinus globosus was studied in 8 habitats in two streams near Ifakara, SE-Tanzania. The relative Bulinus densities were followed monthly. Two different methods for estimating snail densities (man/time vs. palmleaf traps) gave comparable results. Bulinus densities were consta...
journal_title:Acta tropica
pub_type: 杂志文章
doi:
更新日期:1985-06-01 00:00:00
abstract::The surface coat of the protozoan parasite Leishmania affords remarkable protection in the harsh environments encountered within the insect vectors and vertebrate hosts. It also provides specificity for the interaction of these parasites with the cells in the sandfly gut and with the human macrophage. Surprisingly few...
journal_title:Acta tropica
pub_type: 杂志文章,评审
doi:10.1016/0001-706x(93)90029-b
更新日期:1993-05-01 00:00:00
abstract::A key tool for the control, elimination, and eradication of Plasmodium vivax is the development of an effective vaccine. The thrombospondin-related adhesion protein (TRAP) is one of the major sporozoite antigens that plays an important role in the invasion of mosquito salivary glands and hepatocytes by sporozoites. Th...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2017.05.026
更新日期:2017-09-01 00:00:00
abstract::Domestic reinfestations by triatomine bugs were monitored after application of deltamethrin and apparent elimination of Triatoma infestans in Amama and other nearby rural villages, north-west Argentina, from 1992 to 1996. The five methods used were sensor boxes, sheets of pink typing-paper, timed manual catches by a s...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/s0001-706x(98)00096-5
更新日期:1999-03-15 00:00:00
abstract::Anopheles minimus, an important malaria vector of South East Asia, has reappeared in the Singhbum hills, East-Central India where deforestation and DDT residual spraying had reportedly eliminated it during the Malaria Eradication Programme. The species reported has been identified as sibling species A of the An. minim...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2005.05.013
更新日期:2005-10-01 00:00:00
abstract::India and other Southeast Asian countries are severely affected by Japanese encephalitis (JE), one of the deadliest vector-borne disease threat to human health. Several epidemiological observations suggest climate variables play a role in providing a favorable environment for mosquito development and virus transmissio...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2020.105701
更新日期:2020-12-01 00:00:00
abstract::Onchocerca volvulus is a human pathogenic filarial nematode causing chronic onchocerciasis, a disease characterized by chronic skin and eye lesions. Despite attempts to control this infection from many perspectives, it still remains a threat to public health because of adverse effects of available drugs and recent rep...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2012.05.013
更新日期:2012-10-01 00:00:00
abstract::The Fast Agglutination Screening Test (FAST) was employed on sera obtained from an endemic area of visceral leishmaniasis in southwestern Ethiopia, in February 2000. The study involved (i) active case detection among 1575 residents of two villages; and (ii) passive case detection in an outpatient clinic. Sera of 1587 ...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/s0001-706x(02)00063-3
更新日期:2002-08-01 00:00:00
abstract::The antidiarrhoeal effects of the methanol extract of Zizyphus spina-christi stem bark were evaluated in laboratory rodents. Studies on castor oil induced diarrhoea, intraluminal fluid accumulation, and gastrointestinal transit time were carried out. Results obtained revealed that the extract caused a dose dependent p...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/s0001-706x(03)00114-1
更新日期:2003-07-01 00:00:00
abstract::The critical step of Trichinella spiralis infection is that the muscle larvae (ML) are activated to intestinal infective larvae (IIL) which invade the intestinal columnar epithelium to further develop. The IIL excretory/secretory (ES) proteins play an important role in host-parasite interaction. Proteolytic enzymes ar...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2021.105825
更新日期:2021-01-07 00:00:00
abstract::It has been reported a higher seroprevalence of HBV and HCV in leprosy patients than in the general population, but the reasons for these findings are not yet clear. On the other hand, there is evidence that these viruses may influence the onset of leprosy reactional episodes, an important cause of neurological sequel...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2017.04.024
更新日期:2017-08-01 00:00:00
abstract::Strongyloidiasis is widely distributed in the tropical and subtropical areas. Ivermectin is the drug of choice for the treatment. However, the concerns about relying treatment on a single drug make identification of new molecules a priority. Alkylphospholipid analogues, including edelfosine, are a group of synthetic c...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2016.07.001
更新日期:2016-10-01 00:00:00
abstract::From 1982 to 1984 170 children of Kikwawila village (Kilombero district, Tanzania) were followed for nutritional (anthropometric measures, hematocrit, serum retinol, prealbumin, and zinc concentrations), parasitological (malaria parasitemia, urinary schistosomiasis, intestinal parasites) and immunological characterist...
journal_title:Acta tropica
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
abstract::A survey involving 77 individuals living in two savannah villages near Bobo Dioulasso (Burkina Faso, West Africa), was performed in June 1987 (before), August-September (during) and January 1988 after the seasonal transmission. The clinical longitudinal study during the seasonal period permitted us to define three dif...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/0001-706x(92)90064-5
更新日期:1992-04-01 00:00:00
abstract::In accordance with international targets, the Uganda National Malaria Control Strategic Plan established specific targets to be achieved by 2010. For children under five, this included increasing the number of children sleeping under mosquito nets and those receiving a first-line antimalarial to 85%, and decreasing ca...
journal_title:Acta tropica
pub_type: 杂志文章,评审
doi:10.1016/j.actatropica.2015.07.028
更新日期:2015-12-01 00:00:00
abstract::Human visceral (VL, also known as Kala-azar) and cutaneous (CL) leishmaniasis are important infectious diseases affecting countries in East Africa that remain endemic in several regions of Ethiopia. The transmission and epidemiology of the disease is complicated due to the complex life cycle of the parasites and the i...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2015.02.006
更新日期:2015-05-01 00:00:00
abstract::Rabies is a public health problem in the world especially in Côte d'Ivoire. The epidemiological surveillance of rabies revealed 10,197 exposures with 17 deaths in 2015 and 11,481 exposures with 18 deaths in 2016. The management of exposed people was based on WHO's intramuscular (IM) protocols (Essen and Zagreb). The u...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2020.105710
更新日期:2020-12-01 00:00:00
abstract::The persistence of Trypanosoma cruzi in seropositive individuals, previously diagnosed as chronic chagasic patients (CCP), was detected for the first time in biopsies taken from gingival inflammatory foci processed by polymerase chain reaction (PCR). Seven out of 31 (22.5%) gum samples from selected unquestionably CCP...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2010.12.010
更新日期:2011-03-01 00:00:00
abstract::The polyamines, spermine and spermidine--organic polycations that are absolutely required for eukaryotic cell growth--are shown here to function in Trypanosoma cruzi epimastigotes, as protectors of membrane lipoperoxidation by reactive oxygen species generated either by H2O2/Fe2+ or nifurtimox. In vitro, spermine and ...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2006.02.005
更新日期:2006-04-01 00:00:00
abstract::A tick/rickettsial survey in various parts of Switzerland revealed the presence of a new, hitherto undescribed spotted fever group rickettsia ("Swiss agent") in up to 11.7% of I. ricinus collected off vegetation. Infection in ticks was found to be generalized with rickettsiae developing intracellularly and occasionall...
journal_title:Acta tropica
pub_type: 杂志文章
doi:
更新日期:1979-12-01 00:00:00
abstract::Entomopathogenic fungi, especially Metarhizium anisopliae, have potential for integrated control of peridomestic triatomine bugs. However, the high susceptibility of these vectors to fungal infection at elevated ambient humidities decreases in the comparatively dry conditions that often prevail in their microhabitats....
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2011.11.014
更新日期:2012-04-01 00:00:00
abstract::Bashagard is an important malaria endemic area in south of Iran. An epidemiological and entomological survey was performed during 2002-2010. The aim of study was to determine malaria situation, species composition of anopheline mosquitoes and susceptibility status of main vectors to insecticides/larvicides. A total of...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2011.04.017
更新日期:2012-02-01 00:00:00
abstract::Plasmodium vivax merozoite surface protein 1 (PvMSP-1) is a leading malaria vaccine candidate. This protein is processed to give rise to various sized fragments during merozoite maturation. Here, we describe the analysis of genetic diversity in the 42 kDa C-terminal part of this protein among 33 Indian P. vivax isolat...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2008.08.011
更新日期:2008-10-01 00:00:00
abstract::Cameroon lacks the capacity for routine Pneumocystis pneumonia (PcP) diagnosis, thus, the prevalence of Cameroonian exposure to this microbe is unknown. It is known that Pneumocystis infecting different mammalian host species represent diverse phylogenetic backgrounds and are now designated as separate species. The hi...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2009.07.030
更新日期:2009-11-01 00:00:00
abstract::Dicrocoelium dendriticum (Rudolphi, 1819) and Dicrocoelium hospes (Looss, 1907) are recognised to affect the liver of domestic and wild ruminants. A third species, Dicrocoelium orientalis which was described from musk deer in the Baikal region of the former Soviet Union and re-named to Dicrocoelium chinensis (Sudariko...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2007.07.008
更新日期:2007-11-01 00:00:00
abstract::Leishmania (Mundinia) orientalis is a newly described species causing human leishmaniasis in Thailand whose natural vector is unknown. L. orientalis infections in sand flies and/or biting midges under laboratory conditions have not been previously investigated. In this study, the development of L. orientalis in two ex...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2019.105157
更新日期:2019-11-01 00:00:00
abstract::In this study, Aphanius chantrei's behaviour of feeding on mosquito larvae has been determined under experimental conditions. In groups that have different sizes (first group 27 mm; second group 40 mm; third group 47 mm), larvae consumption has increased with length and weight. During daytime, average larvae consumpti...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2007.04.016
更新日期:2007-06-01 00:00:00