Clostridium difficile caused changes in fatty acids profile and resolvin D1 content in plasma of infected patients.

Abstract:

OBJECTIVES:Clostridium difficile infection (CDI) is an acute gastrointestinal infection caused by anaerobic, toxin-producing bacteria. During the course of CDI, there is a general inflammatory state. In order to gain a deeper understanding of the role of fatty acids (FAs) in the pathogenesis of acute infection we analyzed their plasma content in both patients with CDI and controls. METHODS:The study groups included 40 patients with CDI and 40 healthy volunteers. Plasma FA content was analyzed by gas chromatography, resolvin D1 (RvD1) level using ELISA assay, and we assessed the white blood cell (WBC) count, neutrophil count and C-reactive protein (CRP) level. RESULTS:Patients with CDI were characterized by significantly higher values of WBC, neutrophils, platelets and CRP compared with the control group. The saturated FA index was statistically higher and total n-3 FA was significantly decreased in the plasma of CDI patients as compared with the control group. RvD1 content was significantly higher in the control group as compared with patients with CDI. CONCLUSION:In patients with good outcomes, we probably observed the effective resolution of inflammation, as reflected in n-3 FA metabolism and their significant decrease in plasma. This may indicate the therapeutic role of n-3 FA in CDI infection.

authors

Dróżdż M,Gdula-Argasińska J,Biesiada G,Jurczyszyn A,Perucki W,Wołkow P,Borys A,Sroczyńska K,Zając A,Librowski T,Garlicki A,Czepiel J

doi

10.1097/MEG.0000000000001600

subject

Has Abstract

pub_date

2020-03-01 00:00:00

pages

318-324

issue

3

eissn

0954-691X

issn

1473-5687

journal_volume

32

pub_type

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