Duration of brain death and cold ischemia time, but not warm ischemia time, increases expression of genes associated with apoptosis in transplanted kidneys from deceased donors.


:Apoptosis is one of the most important mechanisms leading to kidney graft injury during transplantation. The aim of this study was to assess the expression of genes involved in apoptosis in transplanted kidneys derived from deceased donors (DD) at various stages of the transplant procedure, seventy eight transplanted kidneys procured from 43 DD were included in this study. As a baseline control for gene expressions we used six kidney allografts obtained from living donors (LD). Three core biopsies were performed: biopsy 1--5 minutes before organ perfusion in the donor; biopsy 2--at the end of cold ischemia before kidney implantation; and biopsy 3--30 minutes after reperfusion. Tumor protein p53 (TP53), caspase-3 (CASP3), B-cell lymphoma 2 protein (Bcl2), and heme oxygenase 1 (HO-1) gene expression levels were determined using custom-designed low-density arrays (TaqMan assay). Comparison of gene expression between DD and LD kidneys revealed greater expression of all genes in kidneys from DD in all biopsies; however, only CASP3 expression in biopsy 1 and TP53 expression in biopsy 3 were statistically significant. Prolongation duration of brain death beyond 10 hours in DD resulted in a significantly decreased CASP3 expression in biopsy 1. When the cold ischemia time (CIT) was longer than 24 hours, the expressions of Bcl2, TP53, and CASP3 were significantly higher compared to kidneys with ClT<24 hours. There was no correlation between warm ischemia time and gene expression in biopsy 3. CASP3 and TP53 expression only in biopsy 1 were significantly higher among kidney allografts with delayed (DGF) compared with immediate graft function. In conclusion expression of genes involved in apoptosis was more pronounced in kidney allografts from deceased donors. A prolonged donor brain-death period beyond 10 hours resulted in decreased CASP3 expression. CIT longer than 24 hours was associated with increased expressions of Bcl2, TP53, and CASP3. CASP3 and TP53 expressions were significantly higher among kidneys allografts displaying DGF.


Transplant Proc


lznerowicz A,Chudoba P,Kamińska D,Kościelska-Kasprzak K,Drulis-Fajdasz D,Hałoń A,Janczak D,Boratyńska M,Klinger M,Patrzałek D,Polak WG




Has Abstract


2011-10-01 00:00:00














  • Mycophenolic acid upregulates B7-DC expression on dendritic cells, which is associated with impaired allostimulatory capacity of dendritic cells.

    abstract::In addition to its effects on lymphocytes, mycophenolic acid (MPA) may inhibit the allostimulatory capacity of dendritic cells (DC) via unknown mechanisms. B7-H1 and B7-DC surface markers on DC negatively regulate T-cell responses via the receptor PD-1. In this study, we sought to investigate whether B7-H1 and B7-DC a...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Geng L,Jiang G,Xie H,Fang Y,Dong S,Chen Y,Shen M,Zheng S

    更新日期:2006-06-01 00:00:00

  • The importance of clinical parameters when differentiating cholestatic hepatitis C virus from allograft rejection.

    abstract:BACKGROUND:The exact cause and appropriate treatment for cholestasis following liver transplantation in recipients with hepatitis C virus recurrence (RHCV) are difficult to determine. Our objective was to determine the diagnostic accuracy of clinical and histological parameters in liver transplant recipients with RHCV ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Neff GW,Shire N,Ruiz P,O'Brien C,Garcia M,Dela Garza J,Rudich SR,Reddy KR

    更新日期:2005-12-01 00:00:00

  • Stability of Organ Donor Designations on Driver's Licenses.

    abstract:BACKGROUND:Little is known about the stability of decisions that people make to be organ donors. We sought to determine the rate of stability of organ donor designations on driver's licenses. METHODS:With the use of a cross-sectional study design, we reviewed the health records of 2500 randomly selected primary-care p...

    journal_title:Transplantation proceedings

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    authors: Sehgal NK,Sullivan C,Figueroa M,Pencak JA,Thornton JD

    更新日期:2016-07-01 00:00:00

  • Combined heart and liver transplantation in four adults with familial amyloidosis: experience of a single center.

    abstract::There are few reports of combined heart and liver transplantation (CHLT) for familial amyloidotic polyneuropathy (FAP). The technique for the operation remains to be defined. Four CHLTs were performed for amyloidogenic transthyretin-related (variant Glu89Gln-ATTR Glu89Gln) cardiomyopathy in our center. Patients 1 and ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Nardo B,Beltempo P,Bertelli R,Montalti R,Vivarelli M,Cescon M,Grazi GL,Salvi F,Magelli C,Grigioni F,Arpesella G,Martinelli G,Cavallari A

    更新日期:2004-04-01 00:00:00

  • Long-term prognostic implications of metabolic syndrome in heart transplant recipients.

    abstract:BACKGROUND:Metabolic syndrome (MS) increases the risk of cardiovascular events due to endothelial dysfunction. There are few studies evaluating the impact of MS on the survival of heart transplantation (HTx) patients. AIM:The aim of this study was to study the impact of MS in the early period and on the long-term surv...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Sánchez-Lázaro IJ,Sánchez-Gómez JM,Almenar-Bonet L,Martínez-Dolz L,Muño-Giner B,Portolés-Sanz M,Roselló-Lleti E,Cortés-Vergaz R,Rivera-Otero M,Salvador-Sanz A

    更新日期:2011-07-01 00:00:00

  • Detection of citrate synthase autoantibodies in rats with chronic allograft nephropathy.

    abstract:UNLABELLED:Citrate synthase (CS) is the one of the key enzymes in the citric acid cycle and an important mitochondrial autoantigen. The autoimmune responses against CS have not been studied in chronic allograft nephropathy (CAN). This study investigated the role of specific CS autoantibodies in rats bearing renal allog...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Zhang LY,Lu YP,Yang L,Luo GH,Song J,Li YP

    更新日期:2009-12-01 00:00:00

  • Split liver transplantation in Shiraz Transplant Center.

    abstract:BACKGROUND:Cadaveric organ splitting emerged from an improved understanding of the surgical anatomy of the liver as a possible mechanism to expand the organ pool. In this study, we have reported our first series of split liver transplantations (SLT). MATERIALS AND METHODS:From June 2006 to June 2008, we performed 17 p...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Bahador A,Salahi H,Nikeghbalian S,Dehghani SM,Kakaei F,Sabet B,Kazemi K,Rajaei E,Gholami S,Malek-Hosseini SA

    更新日期:2009-09-01 00:00:00

  • Effect of splenic artery embolization for splenic artery steal syndrome in liver transplant recipients: estimation at computed tomography based on changes in caliber of related arteries.

    abstract:PURPOSE:To estimate the effect of splenic artery embolization (SAE) on blood flow in orthotopic liver transplantation (OLT) recipients with splenic artery steal syndrome (SASS) based on changes in caliber of related arteries upon serial computed tomography (CT) scans. METHODS:Between 2004 and 2007, nine OLT recipients...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Kim JH,Kim KW,Gwon DI,Ko GY,Sung KB,Lee J,Shin YM,Song GW,Hwang S,Lee SG

    更新日期:2011-06-01 00:00:00

  • Taurine protects against ischemia-reperfusion injury in rabbit livers.

    abstract::Ischemia-reperfusion injury is of major clinical relevance during liver transplantation. In the present study, the therapeutic efficacy of taurine to protect against ischemia-reperfusion injury was investigated in rabbit livers. We demonstrated that intravenous injection of taurine attenuated liver dysfunction as evid...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Tong L,Li J,Qiao H,Jiang H,Meng F,Sun X

    更新日期:2006-06-01 00:00:00

  • Valgancyclovir as maintenance therapy for cytomegalovirus retinitis in a lung transplant patient--a case report.

    abstract:UNLABELLED:The purpose of this article is to report the use of valgancyclovir as maintenance therapy for cytomegalovirus (CMV) retinitis in a lung transplanted patient. RESULT:A 30-year-old woman with underlying pulmonary lymphangioleiomyomatosis received a lung transplant 1 year ago. CMV retinitis developed 4 months ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Wang TJ,Lin CP,Yang CM,Lin LL

    更新日期:2004-11-01 00:00:00

  • Nitric oxide levels in the intestines of mice submitted to ischemia and reperfusion: L-arginine effects.

    abstract:OBJECTIVE:Usually an experimental necrotizing enterocolitis experimental model, we Investigated nitric oxide levels in intestinal tissues of newborn mice with or without l-arginine therapy during sessions of ischemia and reoxygenation. METHODS:Twenty-six newborn mice from the Wistar EPM-1 lineage, weighing from 4.5 to...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Cintra AE,Martins JL,Patrício FR,Higa EM,Montero EF

    更新日期:2008-04-01 00:00:00

  • Arterial anastomosis disrupton in two kidney recipients of contaminated grafts from a donor with Gorham's syndrome.

    abstract::Transmission of donor infections to immunosuppressed recipients may produce serious complications. Here, we report two cases of ruptured renal artery pseudoaneurysm within a few months after renal transplantation from a donor with Gorham's syndrome, a rare disease characterized by proliferation of vascular and lymphat...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章,评审


    authors: Saidi RF,Fasola C,El-Ghoroury M,Oh H

    更新日期:2004-06-01 00:00:00

  • Effects of Granulocyte and Monocyte Adsorptive Apheresis in Renal Transplantation Recipients With Concomitant Cytomegalovirus Infection.

    abstract:BACKGROUND:Granulocyte and monocyte adsorptive apheresis (GMAA) is widely used as a treatment for active ulcerative colitis (UC) in Japan. Much attention has been paid to the possibility of GMAA for the treatment and control of cytomegalovirus (CMV) reactivation in patients with refractory UC and concomitant CMV infect...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Naganuma T,Takemoto Y,Iwai T,Kuwabara N,Uchida J,Nakatani T,Kitamura K,Masuda A,Ohmori K,Matsuura M,Nakase H

    更新日期:2016-04-01 00:00:00

  • Difficulties in organ procurement and transplantation in the aboriginal minority people in Taiwan.

    abstract:BACKGROUND:Aboriginal people (AP) are a minority group in Taiwan. Little information on their perspectives on organ transplantation (OT) is available. Their rights for organ donation (OD) and as OT recipients (OTR) are constrained as a vulnerable population in society. This research sought to explore various Highland A...

    journal_title:Transplantation proceedings

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    authors: Shih FJ,Chang YC,Chen CF,Lin FJ,Huang YS,Yan YH,Shih FJ,Chen CR

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  • The outcome of induction therapy with monoclonal antibodies in kidney transplantation among Iranian patients: a prospective study.

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    journal_title:Transplantation proceedings

    pub_type: 杂志文章,随机对照试验


    authors: Naderi GH,Mehraban D,Ganji MR,Jafarpouriani M,Latif AH

    更新日期:2009-09-01 00:00:00

  • Reliability and Accuracy of Clinical Assessment and Digital Image Analysis for Steatosis Evaluation in Discarded Human Livers.

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    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Abudhaise H,Luong TV,Watkins J,Fuller BJ,Davidson BR

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  • Association of hyperglycemia on allograft function in the early period after renal transplantation.

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    pub_type: 杂志文章


    authors: Ganji MR,Charkhchian M,Hakemi M,Nederi GH,Solymanian T,Saddadi F,Amini M,Najafi I

    更新日期:2007-05-01 00:00:00

  • Drug interaction between tacrolimus and ertapenem in renal transplantation recipients.

    abstract::To show drug interactions between tacrolimus and ertapenem, we retrospectively evaluated 13 renal transplant recipients who had been treated with ertapenem for urinary tract infections during prescription of a constant dose. The mean dose of tacrolimus to achieve desired therapeutic concentrations decreased significan...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Bora F,Aliosmanoglu I,Kocak H,Dinckan A,Uslu HB,Gunseren F,Suleymanlar G

    更新日期:2012-12-01 00:00:00

  • Reactivations of cytomegalovirus, human herpes virus 6, and Epstein-Barr virus differ with respect to risk factors and clinical outcome after hematopoietic stem cell transplantation.

    abstract::One hundred two recipients of hematopoietic stem cell transplants (HSCTs) 45, from siblings and 57 from matched unrelated donors, were followed for cytomegalovirus (CMV), human herpes virus (HHV) 6, and Epstein-Barr Virus (EBV) reactivation by quantitative polymerase chain reaction in the context of immunologic recons...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Jaskula E,Dlubek D,Sedzimirska M,Duda D,Tarnowska A,Lange A

    更新日期:2010-10-01 00:00:00

  • Erythropoietin Ameliorates Lung Injury by Accelerating Pulmonary Endothelium Cell Proliferation via Janus Kinase-Signal Transducer and Activator of Transcription 3 Pathway After Kidney Ischemia and Reperfusion Injury.

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    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Zhu M,Wang L,Yang J,Xie K,Zhu M,Liu S,Xu C,Wang J,Gu L,Ni Z,Xu G,Che M

    更新日期:2019-04-01 00:00:00

  • Plasmacytoid dendritic cells: in vivo regulators of alloimmune reactivity?

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    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Abe M,Colvin BL,Thomson AW

    更新日期:2005-11-01 00:00:00

  • Internal jugular versus subclavian vein catheterization for central venous catheterization in orthotopic liver transplantation.

    abstract::The aim of this study was to compare incidence rates of mechanical and infectious complications associated with central venous catheterization via the internal jugular vein (IJV) versus the subclavian vein (SV) among 45 consecutive patients undergoing orthotopic liver transplantation (OLT) between January 2000 and Jun...

    journal_title:Transplantation proceedings

    pub_type: 临床试验,杂志文章


    authors: Torgay A,Pirat A,Candan S,Zeyneloglu P,Arslan G,Haberal M

    更新日期:2005-09-01 00:00:00

  • Cardiovascular and Neurologic Complications in Kidney Transplant Recipients: A Focused Appraisal of Symptoms.

    abstract:BACKGROUND AND OBJECTIVE:After a kidney transplantation, all efforts are focused on graft function. However, cardiovascular and neurologic complications might lead to decreased quality of life and shortened life expectancy. Early recognition of related symptoms might be critical to successfully manage these complicatio...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Dogan U,Yaprak M,Dogan EA,Onac M,Yılmaz VT,Aydinli B

    更新日期:2019-05-01 00:00:00

  • Incidence and risk factors of deep vein thrombosis after liver transplantation.

    abstract:BACKGROUND:Deep venous thrombosis (DVT) occurs in 0.1% of persons per year, affecting 15%-40% of general surgical procedures without prophylaxis. Thromboembolic prophylaxis is not commonly used after orthotopic liver transplantation (LT) owing to the risks of bleeding and coagulopathy. Cirrhosis and the association wit...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Annamalai A,Kim I,Sundaram V,Klein A

    更新日期:2014-12-01 00:00:00

  • Effect of routine insertion of a double-J stent after living related renal transplantation.

    abstract::It is still not clear whether double-J stents (DJS) are of benefit. We sought to determine whether routine prophylactic use of DJS reduced postoperative complications after renal transplantation. We prospectively evaluated 42 living donor renal transplantations performed between September 2001 and September 2003. The ...

    journal_title:Transplantation proceedings

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Moray G,Yagmurdur MC,Sevmis S,Ayvaz I,Haberal M

    更新日期:2005-03-01 00:00:00

  • Prevalence and manifestations of tuberculosis in renal transplant recipients: a single-center experience in Thailand.

    abstract::This retrospective review presented the prevalence and manifestations of tuberculosis among renal transplant recipients in our center between 1987 and mid 2007. The prevalence of tuberculosis was 5/151 (3.3%) recipients with a median age of 49 years (range = 38-55). The median time of diagnosis after transplantation w...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Ruangkanchanasetr P,Natejumnong C,Kitpanich S,Chaiprasert A,Luesutthiviboon L,Supaporn T

    更新日期:2008-09-01 00:00:00

  • Guidelines for prevention and management of complications following kidney transplantation in rats.

    abstract::Kidney transplantation in rats is a useful model for microsurgery, transplantation, and immunology studies. Our aim was to analyze various techniques of kidney transplantation in rats with emphasis on guidelines for the prevention and management of complications. Complications were categorized into general, vascular, ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Pahlavan PS,Mehrabi A,Kashfi A,Soleimani M,Fani-Yazdi SH,Schemmer P,Gutt CN,Friess H,Weitz J,Kraus TW,Büchler MW,Schmidt J

    更新日期:2005-06-01 00:00:00

  • Low-dose dopamine in kidney transplantation.

    abstract:BACKGROUND:The use of low-dose dopamine (LDD; 0.5-2.5 mug/kg/min) in kidney transplant recipients seeks to increase urine output, prevent arterial vasospasm, and reduce the incidence of acute tubular necrosis. The aim of this study was to evaluate the effect of LDD in the early postoperative period (12 hours) among kid...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Ciapetti M,di Valvasone S,di Filippo A,Cecchi A,Bonizzoli M,Peris A

    更新日期:2009-12-01 00:00:00

  • Treatment-resistant recurrent membranoproliferative glomerulonephritis in renal allograft responding to rituximab: case report.

    abstract::We report a case of idiopathic membranoproliferative glomerulonephritis (MPGN) recurring 2 years after a living-unrelated kidney transplantation. The disease was refractory to intravenous immunoglobulin and plasmapheresis. Treatment with 2 doses of rituximab resulted in remission of the disease. The disease relapsed 1...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Farooqui M,Alsaad K,Aloudah N,Alhamdan H

    更新日期:2015-04-01 00:00:00

  • Very Long-term Survival of the Transplanted Kidney-Characteristics of Recipients.

    abstract:BACKGROUND:After kidney transplantation (KTx), donor- and recipient-dependent factors, as well as the immunosuppression protocol, may have an impact long-term graft function. The aim of this retrospective study was to identify and describe recipients from a single center who had their transplanted kidney survive for mo...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Karcz M,Kusztal M,Boratyńska M,Klinger M

    更新日期:2018-01-01 00:00:00