Abstract：
:A refined toxic fraction, Fr-2, from the ethanol extract of Aster tataricus roots was obtained by a toxic tracing isolation. The hepatotoxic effects of Fr-2 were proved by the level elevation of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in mice serum and further confirmed by the histopathological examination on mice liver. A simple method was developed for qualitative analysis of constituents in Fr-2 fraction by high-performance liquid chromatography with diode array detection (HPLC-DAD) and electrospray ionization tandem mass spectrometry (ESI-MS(n)). As a result, 35 compounds were detected and identified in Fr-2. Among them, 28 compounds are pentapeptides, including 18 cyclic and 10 linear ones, and the sum of their peak area occupied about 87% (71.2% for cyclic and 15.8% for linear) of total area of chromatographic peak. Other 7 compounds are phenols, occupied about 5.9% of total area of chromatographic peak. Moreover, the structures of 10 cyclic and 2 linear peptides were reported for the first time. The results suggested that the peptides, especially the cyclic ones, are probably the principal toxic substances in Fr-2 also in A. tataricus roots.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Liu X,Cao P,Zhang C,Xu X,Zhang Mdoi
10.1016/j.jpba.2012.04.034subject
Has Abstractpub_date
2012-08-01 00:00:00pages
51-62eissn
0731-7085issn
1873-264Xpii
S0731-7085(12)00231-2journal_volume
67-68pub_type
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