Abstract:
:The interaction between gabapentin (GP), a neuroleptic drug, and calf-thymus DNA was investigated. Cyclic voltammetry and different spectroscopic methods (UV/vis spectrophotometry, spectroflourimetry and circular dichroism) were used to study the process. The oxidation current of GP was considerably decreased in the presence of calf-thymus DNA. The negative shift of the peak potential in cyclic voltammetry suggested an electrostatic interaction, while spectroscopic methods show minor groove binding as the predominant mode. The values of binding constants obtained from UV absorption, spectrofluorimetry and voltammetric measurements were in close agreement. Standard Gibbs free energy change, ΔG(0), of the interaction was calculated to be -21.99 kJ mol(-1) which indicated the spontaneity of the binding interaction. A hyperchromic effect in the maximum absorption band of DNA as well as quenching in fluorescence experiments were observed in the presence of GP.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Jalali F,Dorraji PSdoi
10.1016/j.jpba.2012.06.005subject
Has Abstractpub_date
2012-11-01 00:00:00pages
598-601eissn
0731-7085issn
1873-264Xpii
S0731-7085(12)00337-8journal_volume
70pub_type
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