Abstract:
BACKGROUND:Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is due to maternal alloimmunization against fetal platelet (PLT) antigens. Antenatal management strategies have been developed to avoid complications such as intracranial hemorrhage. The aim of this study was to set up two reliable, noninvasive fetal genotyping assays to determine the fetal risk in pregnancies in which the father is heterozygous for the offending antigen. This study focused on human PLT antigen (HPA)-1, the most frequently implicated antigen in FNAIT in Caucasians. STUDY DESIGN AND METHODS:Two assays based on cell-free fetal DNA extracted from maternal blood samples and on real-time polymerase chain reaction (QPCR) were developed: an allele-specific QPCR specifically targeting the polymorphic sequence in HPA-1 and the study of the variation in the high-resolution melting curve of amplicons containing the polymorphic region. RESULTS:All results from the 49 samples obtained from 29 pregnant women were consistent with expectations. Six women were compatible with their fetuses (three HPA-1aa women and three HPA-1bb women), 41 HPA-1bb women were incompatible with their fetuses, as were two HPA-1aa women. CONCLUSION:Two fetal PLT genotyping assays on maternal blood samples proved to be reliable as of 15 weeks of gestation, thereby avoiding invasive techniques such as amniocentesis.
journal_name
Transfusionjournal_title
Transfusionauthors
Le Toriellec E,Chenet C,Kaplan Cdoi
10.1111/j.1537-2995.2012.03954.xsubject
Has Abstractpub_date
2013-08-01 00:00:00pages
1755-62issue
8eissn
0041-1132issn
1537-2995journal_volume
53pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract::The biochemical constituents of a 30-year-old sealed bottle of lyophilized plasma were analyzed. The results in this communication show that the process of lyophilization and storage has not seemed to cause any great changes in plasma components, including hormones, enzymes, and proteins. ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1977.17177128892.x
更新日期:1977-01-01 00:00:00
abstract:BACKGROUND:Methods that detect a child's homozygosity by examination of allelic products are insensitive for diagnosing incest because, at a given locus, a homozygous state is expected with a frequency of only 0.25 when parents are first-degree relatives. Furthermore, these methods are not specific if the population co...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1994.34294143949.x
更新日期:1994-02-01 00:00:00
abstract::A 24-year-old, blood-group O Polynesian woman with multiple red blood cell antibodies received a group A, HLA-compatible, sex-matched bone marrow transplantation as treatment for severe aplastic anemia. High retransplantation titers of ABO isohemagglutinins were successfully reduced by repeated plasmaphereses. Despite...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1982.22182154221.x
更新日期:1982-01-01 00:00:00
abstract:BACKGROUND:The Mirasol pathogen reduction technology (PRT) system for plasma is based on a riboflavin (vitamin B(2)) and ultraviolet (UV) light treatment process resulting in pathogen inactivation due to irreversible photo-oxidative damage of nucleic acids. The purpose of this study was to evaluate the in vitro protein...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02517.x
更新日期:2010-04-01 00:00:00
abstract::Forty samples of frozen human plasma were thawed in a 37 C water bath following storage at -80 C for 48 hours. Twenty of these samples were protected by heat-sealed polyethylene overwraps prior to freezing and thawing. The remaining 20 samples were unprotected. None of the protected samples leaked during thawing. The ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1981.21481276002.x
更新日期:1981-07-01 00:00:00
abstract:BACKGROUND:Exposure to blood products during pregnancy carries a potential risk of transfusion transmission of infectious agents. Blood agencies have historically sought optimal deferral and testing strategies to protect blood supplies in part to ensure the protection of pregnant women and their unborn infants. The Zik...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14001
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:Throughout history, blood has been embedded with powerful and paradoxical connotations. In the last decades, a more rational conception based on scientific knowledge has emerged, portraying blood as a "disenchanted" and utilitarian object, hence more apt for donation between strangers. This study aims to exa...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12477
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND:This study investigates the effect of a vasovagal reaction (VVR) or needle reaction (NR) on the risk of stopping as a blood donor, taking into account variables from the Theory of Planned Behavior (TPB). Is stopping risk solely related to the adverse reaction itself, or do the TPB variables play a role as we...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03551.x
更新日期:2012-09-01 00:00:00
abstract:BACKGROUND:Research with primary human white blood cell (WBC) subpopulations requires high quantity, quality, and functionality of peripheral blood mononuclear cells (PBMCs) as a source to further characterize cellular subpopulations such as T and B lymphocytes, monocytes, or natural killer cells. Apart from buffy coat...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14616
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:The 2000 Nationwide Blood Collection and Utilization Survey was designed to assess cellular therapy product services in US blood centers and hospitals. STUDY DESIGN AND METHODS:Questionnaires were returned by 2,040 institutions. Data were analyzed for 30 quantitative variables related to cellular therapy pr...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2003.03286.x
更新日期:2004-04-01 00:00:00
abstract:BACKGROUND:Mobilized peripheral blood is the most common source of CD34+ cells intended for transplantations. The collection and enrichment of CD34+ cells could be affected by various factors and there are some controversies regarding the effects of patient-related factors. The aim of this study was to assess the impac...
journal_title:Transfusion
pub_type: 临床试验,杂志文章
doi:10.1111/trf.13646
更新日期:2016-07-01 00:00:00
abstract::Two blood samples containing autoagglutinins reactive in room temperature tests using low-ionic strength salt (LISS) reagents, but not by conventional saline-albumin or enzyme tests, are reported. The anti-P specificity of both sera, when tested near or diluted in group AB serum, was evident by their failure to agglut...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1982.22382224937.x
更新日期:1982-05-01 00:00:00
abstract:BACKGROUND:The platelet (PLT) Pan Genera Detection test (PGD) is a rapid bacterial detection system used to screen PLTs for bacterial contamination. We report a single center 46-month experience with secondary screening of apheresis PLTs by PGD testing. STUDY DESIGN AND METHODS:Existing testing records of apheresis PL...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12083
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:There has been increased worldwide emphasis on the many benefits of human immunodeficiency virus (HIV) serostatus awareness for both infection prevention and improved treatment outcomes. Previous studies indicate that donors may use blood donation to be tested; the objectives of this analysis were to assess,...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13792
更新日期:2016-11-01 00:00:00
abstract:BACKGROUND:Surgical patients receive platelet concentrates (PCs) for a variety of indications. However, there is limited evidence for efficacy or dosing of PCs. STUDY DESIGN AND METHODS:We performed a retrospective cohort study of surgical patients receiving isolated PC transfusion at a single academic tertiary medica...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15156
更新日期:2019-05-01 00:00:00
abstract:BACKGROUND:Although Creutzfeldt-Jakob disease (CJD) has been shown to be transmissible through blood components in rodent models, no human blood-to-blood transmission has been documented. If blood transmission were possible in humans, persons with hemophilia in the United States would be at higher risk of contracting C...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1998.38998409000.x
更新日期:1998-09-01 00:00:00
abstract:BACKGROUND:Red blood cell (RBC) alloantibodies to nonself antigens may develop after transfusion or pregnancy, leading to morbidity and mortality in the form of hemolytic transfusion reactions or hemolytic disease of the newborn. A better understanding of the mechanisms of RBC alloantibody induction, or strategies to m...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12217
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Access to a safe, adequate blood supply has proven challenging in sub-Saharan Africa, where systemic deficiencies spanning policy, collections, testing, and posttransfusion surveillance have long been recognized. Progress in transfusion safety in the early 2000s was in large part due to intervention by the W...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/trf.14949
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:Unpleasant blood donation-related symptoms may discourage otherwise healthy, altruistic individuals from becoming repeat donors. This study examined a behavioral technique called applied muscle tension (AMT) that might reduce reactions. STUDY DESIGN AND METHODS:A total of 605 donors at mobile clinics were a...
journal_title:Transfusion
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1537-2995.2003.00488.x
更新日期:2003-09-01 00:00:00
abstract::Exchange transfusions using whole blood less than 24 hours old and deglycerolized red cells reconstituted with fresh-frozen plasma were performed in two premature infants for the treatment of hyperkalemia and cardiac arrhythmia. Improvement of their condition was immediate. ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1985.25485273824.x
更新日期:1985-07-01 00:00:00
abstract:BACKGROUND:Hypericin is a potent virucidal agent with activity against a broad range of enveloped viruses and retroviruses. The effective virucidal activity emanates from a combination of photodynamic and lipophilic properties. Hypericin binds cell membranes (and, by inference, virus membranes) and crosslinks virus cap...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1995.35595259149.x
更新日期:1995-05-01 00:00:00
abstract:BACKGROUND:G-CSF-mobilized whole blood (WB) is a cost-reducing and simple alternative for peripheral blood progenitor cell transplantation. Recently, it was demonstrated that mobilized WB supplemented with Leibovitz's L15 medium permitted prolonged preservation of clonogenic cells at ambient temperature. In this study,...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1525-1438.2002.00064.x
更新日期:2002-04-01 00:00:00
abstract:BACKGROUND:Proinflammatory cytokines were measured in the supernatant portion of stored, bacterially contaminated, and/or white cell (WBC)-reduced units of red cells (RBCs). Previous studies from this laboratory and others have shown that cytokines are generated in platelet concentrates during storage. This earlier wor...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1995.35395184274.x
更新日期:1995-03-01 00:00:00
abstract::The research process is a balance between the inherent risks of new discoveries and the risks of research participant safety. Conflicts of interest, inherent to the research process, as well as those introduced by emerging cellular therapies, have the potential to compromise safety. The relationship of trust between t...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/j.1537-2995.2005.00616.x
更新日期:2005-10-01 00:00:00
abstract:BACKGROUND:Anti-Tc(a) detects a high-incidence antigen in the Cromer blood group system. Cromer system antibodies have not usually been associated with hemolytic transfusion reactions or hemolytic disease of the newborn. CASE REPORT:Anti-Tc(a) (initially identified in the patient's serum in 1982) was not detected when...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1999.39090948.x
更新日期:1999-09-01 00:00:00
abstract::Originally pasted on walls and on locations reserved specially for that purpose, the poster is a medium for advertising and promotion to be seen on the streets and in public places. More recently, it has spread, in a smaller format, on dedicated indoor sites: billboards, columns, street furniture, and so forth. For tr...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/j.1537-2995.2011.03422.x
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND:Transfusion reactions to platelet concentrates prepared from buffy coats (BC-PCs) were reviewed to determine the effect of some variables of BC-PC preparation and storage: time of BC storage before BC-PC preparation (1-2 days); time of BC-PC storage before transfusion (1-5 days); no white cell reduction vers...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1997.37597293886.x
更新日期:1997-05-01 00:00:00
abstract:BACKGROUND:Conventional platelet (PLT) storage at room temperature under continuous agitation results in a limited shelf life (5 days) and an increased risk of bacterial contamination. However, both of these aspects can be ameliorated by cold storage. Preliminary work has suggested that PLTs can be cold stored for up t...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15651
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:Improving apheresis technology may lead to an efficient and safe peripheral blood stem cell (PBSC) collection. Recently, the Spectra Optia (Optia, Terumo BCT) was introduced as an automated apheresis instrument, but comparisons with other instruments have been few. This is the first randomized multicenter an...
journal_title:Transfusion
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1111/trf.13777
更新日期:2016-11-01 00:00:00
abstract::Simple methods have been developed for adding and removing glycerol during freeze-preservation with 20 per cent W/V glycerol at minus 150 C, or with 40 per cent W/V glycerol at minus 80 C. A one-step method with a 35 per cent W/V glycerol solution is used to prepare 20 per cent W/V glycerolized red blood cells, and a ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1975.15375160354.x
更新日期:1975-05-01 00:00:00