Abstract：
:As the main catalytic and structural molecules within living systems, proteins are the most likely biomolecules to be affected by radiation exposure. Proteomics, the comprehensive characterization of proteins within complex biological samples, is therefore a research approach ideally suited to assess the effects of radiation exposure on cells and tissues. For comprehensive characterization of proteomes, an analytical platform capable of quantifying protein abundance, identifying post-translation modifications and revealing members of protein complexes on a system-wide level is necessary. Mass spectrometry (MS), coupled with technologies for sample fractionation and automated data analysis, provides such a versatile and powerful platform. In this chapter we offer a view on the current state of MS-proteomics, and focus on emerging technologies within three areas: (1) New instrumental methods; (2) New computational methods for peptide identification; and (3) Label-free quantification. These emerging technologies should be valuable for researchers seeking to better understand biological effects of radiation on living systems.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Van Riper SK,de Jong EP,Carlis JV,Griffin TJdoi
10.1007/978-94-007-5896-4_1subject
Has Abstractpub_date
2013-01-01 00:00:00pages
1-35eissn
0065-2598issn
2214-8019journal_volume
990pub_type
杂志文章,评审abstract：:Neisseria meningitidis lipooligosaccharides (LOSs) are classified into 12 immunotypes. Most LOSs are heterogeneous in having a few components by SDS-PAGE analysis that differ antigenically and chemically. We have utilized a monoclonal antibody that recognizes lacto-N-neotetraose (LNnT) and the lectin, Maackia amurensi...
journal_title：Advances in experimental medicine and biology
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abstract：:A kininogenase resembling glandular kallikrein was partially purified from vascular tissue and characterized. Saline perfused rat tail arteries and veins were homogenized in 0.25 M sucrose containing 10 mM Tris-HCl (pH 7.4). The homogenate was centrifuged at 105,000 X g for 60 min and a vascular kininogenase was purif...
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abstract：:This chapter intends to provide an overview of computer-assisted minimally invasive spine surgery (CAMISS) and its clinical application. Since minimally invasive spine surgery was first brought out, the concept of decreasing the damage to patient was soon become popular. However, without the proper surgical field, the...
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journal_title：Advances in experimental medicine and biology
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doi：
更新日期：2010-01-01 00:00:00
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doi：10.1007/978-1-4899-5181-6_33
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journal_title：Advances in experimental medicine and biology
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更新日期：1987-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/978-1-4615-0135-0_70
更新日期：2003-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4684-5925-8_2
更新日期：1991-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4613-2255-9_11
更新日期：1986-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/978-3-030-10616-4_4
更新日期：2019-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4684-5616-5_45
更新日期：1988-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/978-1-4684-4895-5_44
更新日期：1984-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4757-1419-7_34
更新日期：1980-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2016-01-01 00:00:00
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更新日期：1982-01-01 00:00:00
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更新日期：2008-01-01 00:00:00
abstract：:The characteristic functions of relaxin are associated with female reproductive tract physiology. These include the regulation of biochemical processes involved in remodeling the extracellular matrix of the cervix and vagina during pregnancy and rupture of the fetal membranes at term. Such modifications enable the you...
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abstract：:The chapter begins with an historical perspective of GAPDH isozymes that is juxtaposed to the fact that there is only one somatic functional gene in humans that is virtually identical among the mammalian species. Over the many years of GAPDH research, dozens of labs have reported the existence of multiple forms of GAP...
journal_title：Advances in experimental medicine and biology
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abstract：:Animal research in psychiatry suffers from a poor translational value. It is the same for all other disciplines. Our purpose in this chapter is therefore to highlight all the parameters that can lead to a non-reproducibility of interlaboratory experiments as well as intralaboratory. This is to point out the experiment...
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更新日期：2019-01-01 00:00:00
abstract：:The kisspeptin system is considered to be essential for successful mammalian reproduction. In addition to the Kiss1 peptide, Kiss2, the product of kiss2 (the kiss1 paralogue), has also been shown to activate kisspeptin receptor signaling pathways in nonmammalian species. Furthermore, in nonmammalian species, there are...
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更新日期：2013-01-01 00:00:00
abstract：:Laminins are major components of all basement membranes surrounding nerve or vascular tissues. In particular laminin-111, the prototype of the family, facilitates a large spectrum of fundamental cellular responses in all eukaryotic cells. Laminin-111 is a biomaterial frequently used in research, however it is primaril...
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更新日期：2018-01-01 00:00:00
abstract：:This study has examined the role of supplementing a treatment of diabetic rats with captopril (CAP), metformin (MET) or CAP-MET with the antioxidant amino acid taurine (TAU) on biochemical indices of diabetes-induced metabolic changes, oxidative stress and nephropathy. To this end, groups of 6 male Sprague-Dawley rats...
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pub_type： 杂志文章
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更新日期：2017-01-01 00:00:00
abstract：:Vascular endothelial cells (ECs) and smooth muscle cells (VSMCs) are constantly exposed to hemodynamic forces in vivo, including flow shear stress and cyclic stretch caused by the blood flow. Numerous researches revealed that during various cardiovascular diseases such as atherosclerosis, hypertension, and vein graft,...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-96445-4_4
更新日期：2018-01-01 00:00:00
abstract：:We previously showed that taurine administration contributed to the extension of time to exhaustion through exercise-induced hypoglycemia restraint, and we suggested that the activation of hepatic gluconeogenesis was initiated before the exercise with the taurine administration. We hypothesize that the extension effec...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-981-13-8023-5_10
更新日期：2019-01-01 00:00:00
