DNA damage and heat shock dually regulate genes in Saccharomyces cerevisiae.

abstract：:USF is a family of basic helix-loop transcriptional factors that recognizes DNA-binding sites similar to those of the Myc oncoproteins. Here, various functional domains in the mouse USF2 protein were identified and characterized. Indirect immunofluorescence studies with transiently transfected cells revealed that both...

journal_title：Molecular and cellular biology

authors： Luo X,Sawadogo M

更新日期：1996-04-01 00:00:00

abstract：:There are four members of the myocyte enhancer factor 2 (MEF2) family of transcription factors in vertebrates, MEF2A, -B, -C, and -D, which have homology within a MADS box at their amino termini and an adjacent motif known as the MEF2 domain. These factors activate muscle gene expression by binding as homo- and hetero...

journal_title：Molecular and cellular biology

authors： Molkentin JD,Black BL,Martin JF,Olson EN

更新日期：1996-06-01 00:00:00

abstract：:Fusions between the TRM1 gene of Saccharomyces cerevisiae and COXIV or DHFR were made to examine the mitochondrial targeting signals of N2,N2-dimethylguanosine-specific tRNA methyltransferase [tRNA (m2(2)G)dimethyltransferase]. This enzyme is responsible for the modification of both mitochondrial and cytoplasmic tRNAs...

journal_title：Molecular and cellular biology

authors： Ellis SR,Hopper AK,Martin NC

更新日期：1989-04-01 00:00:00

abstract：:A fragment of the human gene for c-erb-B was used to map homologous sequences in mice. Analysis of somatic cell hybrids and recombinant inbred and congenic mouse strains indicated that this gene, designated Erbb, is closely linked to the gene for alpha-globin on mouse chromosome 11. Several genes controlling hematopoi...

journal_title：Molecular and cellular biology

authors： Silver J,Whitney JB 3rd,Kozak C,Hollis G,Kirsch I

更新日期：1985-07-01 00:00:00

abstract：:The int-1 proto-oncogene is a target for insertional activation of transcription by mouse mammary tumor virus in many murine mammary tumors. Whereas no expression of int-1 is seen in normal mammary tissue, int-1 RNA can be detected in normal mice in the neural tubes of midgestation embryos and in postmeiotic spermatoc...

journal_title：Molecular and cellular biology

authors： Papkoff J

更新日期：1989-08-01 00:00:00

abstract：:The Wnt-1 proto-oncogene is essential for proper development of the midbrain and is expressed in a spatially and temporally restricted manner during central nervous system development in mice. In vitro, the gene is specifically transcribed during the retinoic acid (RA)-induced neuroectodermal differentiation of the P1...

journal_title：Molecular and cellular biology

authors： St-Arnaud R,Moir JM

更新日期：1993-03-01 00:00:00

abstract：:Erythroid Krüppel-like factor (EKLF [KLF1]) is a transcriptional regulator that plays a critical role within a specific subset of hematopoietic cells, particularly in the erythroid lineage and its immediate precursor, the megakaryocyte-erythroid progenitor (MEP). We find that EKLF is posttranslationally modified by su...

journal_title：Molecular and cellular biology

authors： Siatecka M,Xue L,Bieker JJ

更新日期：2007-12-01 00:00:00

abstract：:Mitogen-activated protein kinases (MAPKs) play key roles in differentiation, growth, proliferation, and apoptosis. Although MAPKs have been extensively studied, the precise function, specific substrates, and target genes of each MAPK are not known. These issues could be addressed by sole activation of a given MAPK, e....

journal_title：Molecular and cellular biology

authors： Yaakov G,Bell M,Hohmann S,Engelberg D

更新日期：2003-07-01 00:00:00

abstract：:p53 promotes apoptosis in response to death stimuli by transactivation of target genes and by transcription-independent mechanisms. We recently showed that wild-type p53 rapidly translocates to mitochondria in response to multiple death stimuli in cultured cells. Mitochondrial p53 physically interacts with antiapoptot...

journal_title：Molecular and cellular biology

authors： Erster S,Mihara M,Kim RH,Petrenko O,Moll UM

更新日期：2004-08-01 00:00:00

abstract：:The transcription factor Mcm1 is regulated by adjacent binding of a variety of different factors regulating the expression of cell-type-specific, cell cycle-specific, and metabolic genes. In this work, we investigate a new class of Mcm1-regulated promoters that are cell cycle regulated and peak in late M-early G(1) ph...

journal_title：Molecular and cellular biology

authors： Mai B,Miles S,Breeden LL

更新日期：2002-01-01 00:00:00

abstract：:A murine monoclonal antibody was identified by its ability to induce a reversible antiproliferative effect on a human lymphoma cell line. Immunoprecipitation studies revealed that the antibody reacted with a 26-kilodalton cell surface protein (TAPA-1). A diverse group of human cell lines, including hematolymphoid, neu...

journal_title：Molecular and cellular biology

authors： Oren R,Takahashi S,Doss C,Levy R,Levy S

更新日期：1990-08-01 00:00:00

abstract：:Histone H3 lysine 9 (H3K9) methylation has broad roles in transcriptional repression, gene silencing, maintenance of heterochromatin, and epigenetic inheritance of heterochromatin. Using Xenopus laevis oocytes, we have previously shown that targeting G9a, an H3K9 histone methyltransferase, to chromatin increases H3K9 ...

journal_title：Molecular and cellular biology

authors： Stewart MD,Sommerville J,Wong J

更新日期：2006-09-01 00:00:00

abstract：:To determine whether regulation of c-myc proteins occurs during the differentiation of murine erythroleukemia cells, we examined c-myc protein synthesis and accumulation throughout dimethyl sulfoxide (DMSO)- or hypoxanthine-induced differentiation. c-myc protein expression exhibited an overall biphasic reduction, with...

journal_title：Molecular and cellular biology

authors： Spotts GD,Hann SR

更新日期：1990-08-01 00:00:00

abstract：:Genetic and biochemical evidence suggests that the Ras protooncogene product regulates the activation of the Raf kinase pathway, leading to the proposal that Raf is a direct mitogenic effector of activated Ras. Here we report the use of a novel competition assay to measure in vitro the relative affinity of the c-Raf-1...

journal_title：Molecular and cellular biology

authors： Chuang E,Barnard D,Hettich L,Zhang XF,Avruch J,Marshall MS

更新日期：1994-08-01 00:00:00

abstract：:The mechanism(s) and site(s) of action of volatile inhaled anesthetics are unknown in spite of the clinical use of these agents for more than 150 years. In the present study, the model eukaryote Saccharomyces cerevisiae was used to investigate the action of anesthetic agents because of its powerful molecular genetics....

journal_title：Molecular and cellular biology

authors： Keil RL,Wolfe D,Reiner T,Peterson CJ,Riley JL

更新日期：1996-07-01 00:00:00

abstract：:p300 and the closely related CREB binding protein (CBP) are transcriptional adaptors that are present in intracellular complexes with TATA binding protein (TBP) and bind to upstream activators including p53 and nuclear hormone receptors. They have intrinsic and associated histone acetyltransferase activity, suggesting...

journal_title：Molecular and cellular biology

authors： Dallas PB,Cheney IW,Liao DW,Bowrin V,Byam W,Pacchione S,Kobayashi R,Yaciuk P,Moran E

更新日期：1998-06-01 00:00:00

abstract：:The 12p13 ETV6 (TEL) gene is frequently targeted by chromosomal translocations in human malignancies, resulting in the formation of oncogenic ETV6 gene fusions. Many of the known partner genes encode protein tyrosine kinases (PTKs), generating fusion proteins that function as chimeric PTKs. ETV6-NTRK3 (EN), comprised ...

journal_title：Molecular and cellular biology

authors： Tognon CE,Mackereth CD,Somasiri AM,McIntosh LP,Sorensen PH

更新日期：2004-06-01 00:00:00

abstract：:We have constructed a size variant of the Drosophila hsp28 gene by deleting 207 base pairs of the protein coding region, beginning 33 base pairs downstream of the ATG protein initiation codon. After transformation of Drosophila melanogaster rosy (ry506) flies with this altered gene, using the P transposable element sy...

journal_title：Molecular and cellular biology

authors： Hoffman EP,Corces VG

更新日期：1984-12-01 00:00:00

abstract：:Enhancer elements modulate promoter activity over vast chromosomal distances, and mechanisms that ensure restrictive interactions between promoters and enhancers are critical for proper control of gene expression. The human beta-globin locus control region (LCR) activates expression of five genes in erythroid cells, i...

journal_title：Molecular and cellular biology

authors： Shimotsuma M,Matsuzaki H,Tanabe O,Campbell AD,Engel JD,Fukamizu A,Tanimoto K

更新日期：2007-08-01 00:00:00

abstract：:Broken chromosomes can be repaired by several homologous recombination mechanisms, including gene conversion and break-induced replication (BIR). In Saccharomyces cerevisiae, an HO endonuclease-induced double-strand break (DSB) is normally repaired by gene conversion. Previously, we have shown that in the absence of R...

journal_title：Molecular and cellular biology

authors： Signon L,Malkova A,Naylor ML,Klein H,Haber JE

更新日期：2001-03-01 00:00:00

abstract：:The phosphoinositide phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] regulates the activity of many actin-binding proteins and as such is an important modulator of cytoskeleton organization during cell migration, for example. In migrating cells actin remodeling is tightly regulated and localized; therefore, how the ...

journal_title：Molecular and cellular biology

authors： Kisseleva M,Feng Y,Ward M,Song C,Anderson RA,Longmore GD

更新日期：2005-05-01 00:00:00

abstract：:The histone H1(0)-encoding gene is expressed in vertebrates in differentiating cells during the arrest of proliferation. In the H1(0) promoter, a specific regulatory element, which we named the H4 box, exhibits features which implicate a role in mediating H1(0) gene expression in response to both differentiation and c...

journal_title：Molecular and cellular biology

authors： Lemercier C,Duncliffe K,Boibessot I,Zhang H,Verdel A,Angelov D,Khochbin S

更新日期：2000-09-01 00:00:00

abstract：:We find that immediately following transcript initiation, RNA polymerase II pauses at several locations even in the presence of relatively high (200 microM) levels of nucleoside triphosphates. Strong pauses with half-lives of >30 s were observed at +7, +18/19, and about +25 on the template used in these experiments. W...

journal_title：Molecular and cellular biology

authors： Pal M,Luse DS

更新日期：2002-01-01 00:00:00

abstract：:LEU3 of Saccharomyces cerevisiae encodes an 886-amino-acid polypeptide that regulates transcription of a group of genes involved in leucine biosynthesis and has been shown to bind specifically to a 114-base-pair DNA fragment of the LEU2 upstream region (P. Friden and P. Schimmel, Mol. Cell. Biol. 7:2707-2717, 1987). W...

journal_title：Molecular and cellular biology

authors： Friden P,Schimmel P

更新日期：1988-07-01 00:00:00

abstract：:The Drosophila melanogaster Myb-MuvB/dREAM complex (MMB/dREAM) participates in both the activation and repression of developmentally regulated genes and origins of DNA replication. Mutants in MMB subunits exhibit diverse phenotypes, including lethality, eye defects, reduced fecundity, and sterility. Here, we used P-el...

journal_title：Molecular and cellular biology

authors： Lewis PW,Sahoo D,Geng C,Bell M,Lipsick JS,Botchan MR

更新日期：2012-08-01 00:00:00

abstract：:Glucose and oxygen are two of the most important molecules transferred from mother to fetus during eutherian pregnancy, and the metabolic fates of these nutrients converge at the transport and metabolism of pyruvate in mitochondria. Pyruvate enters the mitochondrial matrix through the mitochondrial pyruvate carrier (M...

journal_title：Molecular and cellular biology

authors： Bowman CE,Zhao L,Hartung T,Wolfgang MJ

更新日期：2016-07-14 00:00:00

abstract：:Cholera toxin (CT) activates expression of two immediate-early response genes (JE and TIS10) in quiescent BALB/c 3T3 cells. Increases in cyclic AMP (cAMP) levels in response to CT are likely responsible for the induction of TIS10 gene expression, since treatment with 8-Br-cAMP and increasing the intracellular levels o...

journal_title：Molecular and cellular biology

authors： Qureshi SA,Alexandropoulos K,Joseph CK,Spangler R,Foster DA

更新日期：1991-01-01 00:00:00

abstract：:Several ETS transcription factors, including ELF4/MEF, can function as oncogenes in murine cancer models and are overexpressed in human cancer. We found that Elf4/Mef activates Mdm2 expression; thus, lack of or knockdown of Elf4/Mef reduces Mdm2 levels in mouse embryonic fibroblasts (mef's), leading to enhanced p53 pr...

journal_title：Molecular and cellular biology

authors： Sashida G,Liu Y,Elf S,Miyata Y,Ohyashiki K,Izumi M,Menendez S,Nimer SD

更新日期：2009-07-01 00:00:00

abstract：:Using a subtractive cloning scheme on cDNA prepared from primary pro-B and pre-B cells, we identified several genes whose products regulate apoptosis. We further characterized one of these genes, encoding protein kinase Ceta (PKCeta). PKCeta transcripts were readily detected in pro-B cells but were absent in pre-B cel...

journal_title：Molecular and cellular biology

authors： Morrow TA,Muljo SA,Zhang J,Hardwick JM,Schlissel MS

更新日期：1999-08-01 00:00:00

abstract：:The gene responsible for multidrug resistance (mdr), which encodes the P-glycoprotein, is a member of a multigene family. We have identified distinct mdr gene transcripts encoded by three separate mdr genes in the mouse. Expression levels of each mdr gene are dramatically different in various mouse tissues. Specific m...

journal_title：Molecular and cellular biology

authors： Croop JM,Raymond M,Haber D,Devault A,Arceci RJ,Gros P,Housman DE

更新日期：1989-03-01 00:00:00