Abstract：
:Free-living amoebae (FLA) are protozoa ubiquitously found in nature. As some species or strains of these FLA are pathogenic for humans and animals, they represent objects of medical and parasitological research worldwide. Storage of valuable FLA strains in laboratories is often time- and energy-consuming and expensive. The shipment of such strains as frozen stocks is cumbersome and challenging in terms of cooling requirements as well as of transport regulations. To overcome these difficulties and challenges in maintenance and transport, we present a new method to generate lyophilised samples of non-cyst-forming FLA (Ripella (Vannella) spp.) and cyst-forming FLA (Acanthamoeba spp.) strains which guarantees a simple mechanism for long-term storage at ambient temperature, as well as easy handling and/or shipment. The survival rate of all FLA lyophilisates after short-term storage (2 months) was comparable to the survival rate of freeze cultures of the respective strains. Furthermore, the viability of Acanthamoeba spp. cysts after storage for 29 months was 20 to 40% following lyophilisation and rehydration, with strain variation.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Balczun C,Scheid PLdoi
10.1007/s00436-018-6029-xsubject
Has Abstractpub_date
2018-10-01 00:00:00pages
3333-3336issue
10eissn
0932-0113issn
1432-1955pii
10.1007/s00436-018-6029-xjournal_volume
117pub_type
杂志文章abstract：:The experimental transmission of a recently identified new Theileria (China 1) species pathogenic for sheep and goats in northern China is described. Haemaphysalis qinghaiensis nymphs and adults developed from larvae and nymph engorged on sheep infected with Theileria sp. (China 1) were able to respectively transmit t...
journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2007-08-01 00:00:00
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journal_title：Parasitology research
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pub_type： 杂志文章
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pub_type： 杂志文章,meta分析,评审
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journal_title：Parasitology research
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journal_title：Parasitology research
pub_type： 杂志文章,随机对照试验
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更新日期：2007-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2015-04-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3382-7
更新日期：2013-06-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050106
更新日期：1996-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2017-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：2018-03-01 00:00:00
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更新日期：2013-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-06-01 00:00:00
