Abstract：
OBJECTIVE:To compare the results of polymerase chain reaction (PCR) and immunologic methods for the detection of nanobacteria (NB) in the expressed prostatic secretions (EPSs) of patients with type-III prostatitis. METHODS:In total, 150 patients with type-III prostatitis for whom conventional clinical treatment had failed were selected from September 2009 to April 2010. The EPS of each patient was divided into 3 parts, which were used for PCR analysis, indirect immunofluorescence staining (IIFS), and culture and subsequent indirect immunofluorescence staining (CIIFS). RESULTS:PCR analysis has a higher sensitivity than IIFS for the detection of NB in EPSs. Of 83 CIIFS-positive EPS samples, 79 (95.2%) were positive by PCR. Of 67 EPS samples that were negative by CIIFS, 60 (89.6%) were negative by PCR. The sensitivity of PCR for the detection of NB compared with the CIIFS method was 95.2%, with a specificity of 89.6%. The positive predictive value was 91.9%, and the negative predictive value was 93.8%. A comparative evaluation showed no statistically significant difference between PCR and CIIFS in the detection of NB in EPSs. A strong agreement in the positive and the negative results obtained by PCR and CIIFS for NB detection was found for all EPS samples. CONCLUSION:PCR analysis has a higher sensitivity than IIFS for NB detection in type-III prostatitis. PCR can detect nanobacterial infection in type-III prostatitis equally well as CIIFS and offers significant advantages for the rapid, simple, and economical detection of nanobacterial infection in type-III prostatitis.
journal_name
Urologyjournal_title
Urologyauthors
Zheng J,Tang J,Yin S,Shen X,Zhou Zdoi
10.1016/j.urology.2014.05.038subject
Has Abstractpub_date
2014-09-01 00:00:00pages
731.e9-13issue
3eissn
0090-4295issn
1527-9995pii
S0090-4295(14)00576-7journal_volume
84pub_type
杂志文章相关文献
UROLOGY文献大全abstract：OBJECTIVES:To evaluate whether tamsulosin, as an alpha(1)-blocker, was effective for the treatment of steinstrasse in the lower ureter after shock wave lithotripsy. METHODS:A total of 67 patients (43 men and 24 women) with steinstrasse in the lower portion of the ureters were randomly divided into two groups. Only hyd...
journal_title：Urology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.urology.2005.05.029
更新日期：2005-11-01 00:00:00
abstract：OBJECTIVE:To quantify the relationship between the number of Twitter mentions and the number of academic citations a urologic publication receives. MATERIALS AND METHODS:Two hundred and thirteen papers from 7 prominent urologic journals were examined 37 months after publication. Articles were evaluated with 2 citation...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2018.08.041
更新日期：2019-01-01 00:00:00
abstract：OBJECTIVE:To review the robustness of hydronephrosis literature with the application of fragility index (FI) and fragility quotient (FQ) calculations. METHODS:A literature review was conducted using Pubmed, Medline, and Ovid for "hydronephrosis" and associated terms and we included all studies with at least 2 groups b...
journal_title：Urology
pub_type： 杂志文章,评审
doi：10.1016/j.urology.2019.03.045
更新日期：2019-11-01 00:00:00
abstract：OBJECTIVES:There are large interindividual differences in response to medical therapy for men with benign prostatic hyperplasia. Selection of patients for alpha-blocker versus hormonal treatment is often based more on assumptions than on well-documented knowledge. A more scientifically based decision of therapy has a p...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/s0090-4295(00)00629-4
更新日期：2000-08-01 00:00:00
abstract：OBJECTIVES:To report our contemporary experience with ureterocalicostomy to determine whether the indications or results have changed in modern practice. Ureterocalicostomy is a well-established treatment for patients with complicated ureteropelvic junction (UPJ) obstruction and other forms of proximal ureteral obstruc...
journal_title：Urology
pub_type： 杂志文章,评审
doi：10.1016/j.urology.2004.08.024
更新日期：2005-01-01 00:00:00
abstract：OBJECTIVES:Hormone-independent and cytotoxic drug-resistant tumor growth in osteoblastic metastases defines poor survival in patients with advanced prostate cancer. Therefore, we analyzed the ability of human osteoblast-like cells (MG-63 cells) and MG-63 conditioned media (MG-63 CM) to protect PC-3 human prostate cance...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/s0090-4295(98)00182-4
更新日期：1998-08-01 00:00:00
abstract：:Overactive bladder (OAB) can be caused by a variety of conditions. We believe that cystometrography (CMG) is an essential part of the diagnostic evaluation, both in defining underlying pathophysiology and directing treatment. Essential to the diagnosis of OAB syndrome is some combination of urinary frequency, urgency,...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/s0090-4295(02)01791-0
更新日期：2002-11-01 00:00:00
abstract：OBJECTIVES:To (1) determine if patient age and total prostate-specific antigen (PSA) levels could enhance the ability of percent free PSA to distinguish prostate cancer from benign prostate disease within the 4.0 to 20 ng/mL total PSA range; (2) define the probability of prostate cancer based on patient age, total PSA,...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/s0090-4295(98)00205-2
更新日期：1998-09-01 00:00:00
abstract：OBJECTIVES:Frequently, statistically significant prognostic factors are reported in published studies with suggestions that disease management should be modified. However, the clinical relevance of such factors is rarely quantified. We evaluated the accuracy of predicting the need for invasive treatment among patients ...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2005.11.049
更新日期：2006-05-01 00:00:00
abstract：:Ureteral extension of childhood renal tumors is exceedingly rare. Hematuria, hydronephrosis, poorly or nonfunctioning kidney should raise suspicion for ureteral tumor extension. Identifying ureteral extension on preoperative imaging or intraoperatively before ureteral transection is crucial to obtain good oncological ...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2020.04.113
更新日期：2020-09-01 00:00:00
abstract：:A study of 26 patients with primary hemospermia has been made. When primary hemospermia was the only symptom, urologic investigation was normal. It appears that such patients do not require detailed investigation whereas those presenting with hemospermia and other urologic symptoms require further investigations appro...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(75)90757-8
更新日期：1975-09-01 00:00:00
abstract：:Urinary retention and the possible consequence of infection after total joint replacement is an important subject to urologists and orthopedic surgeons. A prospective, randomized study was performed in 77 elderly female patients with total joint replacement to test whether twenty-four-hour catheterization perioperativ...
journal_title：Urology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0090-4295(88)90381-0
更新日期：1988-09-01 00:00:00
abstract：:Sacral neuromodulation (SNM) has been used off-label in the United States for over a decade in the pediatric population. Many published studies have demonstrated efficacy with SNM in this population; however, a significant number of children with refractory bowel bladder dysfunction (BBD) also have underlying comorbid...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2016.03.006
更新日期：2016-08-01 00:00:00
abstract：OBJECTIVES:We undertook this study to establish criteria for adrenalectomy in patients with renal cell carcinoma. METHODS:We retrospectively reviewed the records of 162 patients undergoing radical nephrectomy from 1979 to 1993 at University Hospitals of Cleveland. Simultaneous ipsilateral adrenalectomy was performed i...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/S0090-4295(96)00388-3
更新日期：1997-01-01 00:00:00
abstract：OBJECTIVE:To investigate the association between urinary neurotrophin levels, maximum flow rate (Qmax) variation, and the appearance of urgency in women with stress urinary incontinence (SUI) after a midurethral sling (MUS) procedure. MATERIALS AND METHODS:Thirty-one women with SUI were treated with a MUS. One year la...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2016.08.048
更新日期：2017-01-01 00:00:00
abstract：:Rhabdomyosarcoma (RMS) is the most common soft-tissue sarcoma of childhood, while RMS from the urinary tract has rarely been reported. Aspects of the manifestation, diagnosis, and treatment of these tumors are discussed in the case of a girl with rapidly progressive RMS from the urethral tract. She was treated with a ...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2007.03.022
更新日期：2007-06-01 00:00:00
abstract：OBJECTIVE:To investigate the role of fatty acid synthase (FASN) in bladder transitional cell carcinoma (BTCC). METHODS:FASN expression was investigated in non-muscle-invasive BTCC tissue specimens by immunohistochemistry and BTCC cell lines by Western blot. After treatment with FASN-siRNA or FASN inhibitor cerulenin (...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2012.02.046
更新日期：2012-08-01 00:00:00
abstract：:We undertook a prospective study to evaluate the effects of hemodialysis on serum prostate-specific antigen (PSA) in 26 male patients with end-stage renal disease as a clinical model for assessing the role of the kidney in PSA clearance. Patients ranging in age from fifty-one to eighty-three years (mean 64.8 years) un...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(93)90365-h
更新日期：1993-10-01 00:00:00
abstract：:A case of basaloid carcinoma of the right epididymis in a 22-year-old Saudi man is described. The tumor presented as a painless swelling of 2 months' duration. Right inguinal orchiectomy has been performed. Microscopically, the stroma was widely infiltrated by tumor cells, with marked apoptosis and necrosis. The tumor...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2004.12.061
更新日期：2005-06-01 00:00:00
abstract：OBJECTIVE:To analyze and compare the etiological uropathogens and the susceptibility profile findings on urine culture between sporadic cases of community-acquired, uncomplicated urinary tract infection (UTI) and recurrent UTI cases in women. MATERIALS AND METHODS:We retrospectively analyzed the clinical data of 1629 ...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2015.05.033
更新日期：2015-09-01 00:00:00
abstract：OBJECTIVES:To obtain accurate spatial information on cancer distribution with a novel prostate biopsy regimen. METHODS:From 2003 to 2005, 265 patients underwent a three fan-shaped biopsy (3FSB) technique of 12 to 14 cores and sextant biopsy (SB) simultaneously. When both techniques had positive findings, and radical p...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2006.08.1065
更新日期：2006-12-01 00:00:00
abstract：OBJECTIVES:To determine the feasibility of routinely performing internal urethrotomy for anterior urethral stricture under intracorpus spongiosum anesthesia in an outpatient setting. METHODS:In a consecutive series of 23 patients with anterior urethral stricture, a dosage of 3 mL of 1% lidocaine was slowly injected in...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/s0090-4295(02)01732-6
更新日期：2002-08-01 00:00:00
abstract：:Schwannoma of the penis is a rare tumor. We report a case of penile schwannoma in a young man who was accurately diagnosed preoperatively with the help of gray-scale and color flow Doppler ultrasonography, and confirmed by magnetic resonance imaging. The tumor was successfully resected to completion, with preservation...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2007.05.003
更新日期：2007-08-01 00:00:00
abstract：OBJECTIVES:To examine the effect of 5-aza-2'-deoxycytidine (DAC), a DNA methyltransferase inhibitor, on the growth of renal cell carcinoma (RCC) and examine the synergistic growth suppression by DAC and chemotherapeutic agents. METHODS:The synergy of DAC and chemotherapeutic agents against RCC cell lines was analyzed ...
journal_title：Urology
pub_type： 历史文章,杂志文章
doi：10.1016/j.urology.2007.02.026
更新日期：2007-05-01 00:00:00
abstract：OBJECTIVES:To examine the effects of bicalutamide (Casodex), a pure antiandrogen with high specificity for the androgen receptor, on insulin-like growth factor binding protein (IGFBP) expression and apoptotic regression of the rat ventral prostate. METHODS:Rats were treated daily with 10 mg/kg body weight bicalutamide...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/s0090-4295(99)00421-5
更新日期：1999-12-01 00:00:00
abstract：OBJECTIVES:To determine the value of mid-infrared spectroscopy (MIRS) of ureteral stent encrustations in predicting urinary stone composition. METHODS:A retrospective study analyzed the composition of stent encrustations and urinary stones by MIRS in patients who had had a stent for ureteral obstruction between 2001 a...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2005.03.054
更新日期：2005-08-01 00:00:00
abstract：:A new operative technique using synthetic, absorbable mesh for grafting a urethral defect was applied in 7 mongrel male dogs. The ventral half of the urethral circumference with its surrounding corpus spongiosum was excised for a length of 3 to 4 cm. A Dexon mesh of the same dimensions, woven in our laboratory from po...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(83)90123-1
更新日期：1983-01-01 00:00:00
abstract：OBJECTIVES:To assess the capacity of several clinical and needle biopsy pathologic parameters to predict insignificant and advanced prostate carcinoma (CaP) in radical prostatectomy tissue from men enrolled in a prostate-specific antigen screening program. METHODS:We captured multiple clinical variables and measures o...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2004.04.008
更新日期：2004-09-01 00:00:00
abstract：OBJECTIVES:To compare the prevalence, symptoms, and pathologic changes related to chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) with significant prostatitis-like symptoms between 2 altitude areas in northwest China. METHODS:From October 2009 to March 2010 community male residents at 3000 m (Delingha) and ...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2011.04.066
更新日期：2011-11-01 00:00:00
abstract：OBJECTIVES:To investigate the clinical efficacy of a caudal block compared with periprostatic local anesthesia for transrectal ultrasound-guided multicore prostate needle biopsy. METHODS:A total of 100 patients who had undergone initial prostate biopsy using a systematic five-region biopsy by a single operator were in...
journal_title：Urology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.urology.2006.02.025
更新日期：2006-08-01 00:00:00
