Abstract:
:Nucleic acid aptamers find widespread use as targeting and sensing agents in nature and biotechnology. Their ability to bind an extensive range of molecular targets, including small molecules, proteins, and ions, with high affinity and specificity enables their use in diverse diagnostic, therapeutic, imaging, and gene-regulatory applications. Here, we describe methods for characterizing aptamer kinetic and equilibrium binding properties using a surface plasmon resonance-based platform. This aptamer characterization platform is broadly useful for studying aptamer-ligand interactions, comparing aptamer properties, screening functional aptamers during in vitro selection processes, and prototyping aptamers for integration into nucleic acid devices.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Chang AL,McKeague M,Smolke CDdoi
10.1016/B978-0-12-801122-5.00019-2subject
Has Abstractpub_date
2014-01-01 00:00:00pages
451-66eissn
0076-6879issn
1557-7988pii
B978-0-12-801122-5.00019-2journal_volume
549pub_type
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